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All Journal Jurnal Ternak Tropika Tropical Animal Science Journal
S. Wahjuningsih
Bagian Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya
Agriculture, Biological Sciences & Forestry Energy

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SUPLEMENTASI FETAL BOVINE SERUM (FBS) TERHADAP PERTUMBUHAN IN VITRO SEL FOLIKEL KAMBING PE Rahayu, S.N; Wahjuningsih, S.
Jurnal Ternak Tropika Vol 12, No 1 (2011): Ternak Tropika
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (26.517 KB)

Abstract

Tujuan penelitian ini adalah untuk mengetahui pengaruh penggunaansuplementasi FBS dengan berbagai konsentrasi pada medium kultur Tissue CultureMedium-199 (TCM-199) dengan variasi waktu inkubasi terhadap kompetensipertumbuhan folikel kambing PE. Materi Penelitian adalah folikel kambing yangdiperoleh melalui isolasi ovarium kambing, TCM-199 dan FBS. Analisa datamenggunakan uji t berpasangan dengan menggunakan 3 perlakuan suplementasi FBS(0%, 0,1% dan 1%) dan inkubasi selama 6 hari. Folikel yang digunakan dalam kulturdikelompokkan menjadi 2 ukuran yaitu besar (4mm) dan kecil (2-<4mm). Variabelpengamatan yang digunakan adalah pertumbuhan folikel. Pengamatan dilakukan padahari ke 0 dan hari ke 6. Hasil penelitian menunjukkan bahwa suplementasi FBS padakultur folikel secara IVG memberikan pengaruh nyata (P<0,05) terhadap pertumbuhanfolikel, yaitu pada folikel besar dengan suplementasi FBS 1% dan folikel kecil dengansuplementasi FBS 0,1%.Kata Kunci : Fetal Bovine Serum, Folikel, Pertumbuhan In Vitro, KambingFETAL BOVINE SERUM (FBS) SUPPLEMENTATION WITHINCUBATION TIME VARIATION TO FOLLICLE GROWTH COMPETENCEOF PERANAKAN ETAWAH (PE) GOATABSTRACTThe aims of this research was to know the effect of FBS supplements usagewith any concentration of Tissue Culture Medium-199 (TCM-199) culture mediumwith incubation time variation to follicle growth competence of Peranakan Etawah(PE) goat. The material of this research were goat follicle, TCM-199 and FBS. Themethod of this research use t test which used 3 treatment with incubation 6 days. Thefollicle that used in culture were classified into 2 groups: large (>4mm) and small (2-<4mm). The observation variable were used follicle growth. The result of this researchare showed that FBS supplements in follicle culture according to IVG gave thesignificant different into follicle growth (P<0,05) that is large follicle with 1% FBSsupplements and small follicle with 0,1% FBS supplements. It can be concluded thatFBS supplements and length of culture in IVG follicle had the significant influenceon the growth of follicles.Keywords: Fetal Bovine Serum,Follicle, In Vitro Growth,Goat
The Quality of Gaga Roosters Semen During Cold Storage Using a Diluent Supplemented with Sorbitol Khaeruddin; Ciptadi, G.; Yusuf, M.; Fattah, A. H.; Junaedi; Syamsuryadi, B.; Wahjuningsih, S.
Tropical Animal Science Journal Vol. 47 No. 4 (2024): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2024.47.4.436

Abstract

This study aimed to examine the effect of adding different concentrations of sorbitol to the Ringer’s lactate-egg yolk (RLEY) diluent on the quality of Gaga roosters’ semen during cold storage. A completely randomized design was used with 10 replicates and 4 levels of diluent treatment, including control, 1%, 2%, and 3% sorbitol. Semen was collected using a massage method, followed by fresh semen evaluation and dilution with a ratio of 1:5. Semen was stored at a cold temperature (5 ºC) and observed after 0, 24, 48, and 72 hours. The variables observed were semen pH, motility, kinematics, viability, plasma membrane integrity, acrosome integrity, malondialdehyde (MDA), and intracellular calcium ion (Ca2+) concentrations of sperm. All data were analyzed using analysis of variance (ANOVA). Subsequently, the evaluation of sperm mitochondrial activity and DNA damage was carried out, and the data were analyzed using the T-test. The results showed that treatment with sorbitol in the diluent had no effect on semen pH, kinematics (except straightness), acrosomal cap integrity, DNA damage, mitochondrial activity, and MDA concentration. However, sorbitol concentrations significantly affect (p<0.05) total motility, progressive motility, static motility, STR, viability, plasma membrane integrity, and intracellular Ca2+. In conclusion, the addition of 1-3% sorbitol preserves the quality of Gaga roosters’ semen during 72 hours of cold storage.
Co-Authors Fattah, A. H. G. Ciptadi JUNAEDI Khaeruddin M. Yusuf S.N Rahayu Syamsuryadi, B.