Toxicity Test of Windu Shrimp (Penaeus monodon) Skin Chitosan With Brine Shrimp Lethality Test Method

Dina Suciati Saragih (Department of Pharmacy, Universitas Muslim Nusantara Al Washliyah)
Ridwanto Ridwanto (Department of Pharmacy, Universitas Muslim Nusantara Al Washliyah)
Anny Sartika Daulay (Department of Pharmacy, Universitas Muslim Nusantara Al Washliyah)
Dikki Miswanda (Department of Pharmacy, Universitas Muslim Nusantara Al Washliyah)
Haris Munandar Nasution (Department of Pharmacy, Universitas Muslim Nusantara Al Washliyah)

Indonesian Journal of Chemical Science and Technology
Vol 5, No 2 (2022): JULI 2022

Chitosan is a modification of chitin compounds that are widely found in the outer skin of crustacean animals such as shrimp and crabs. This research includes isolation of chitin and chitosan: deproteination, demineralization, depigmentation and deacetylation namely transformation of chitin into chitosan, characterization of chitosan, FTIR, and chitosan toxicity test with five concentrations of test solution, namely 100 g/ml, 250 g/ml, 500 g/ml, 750 g/ml and 1000 g/ml using the BSLT method by looking at the number of deaths of Artemia salina L larvae (LC50). The results of tiger prawn shell chitosan (Penaeus monodon) obtained the % degree of deacetylation of 60%. The results of the toxicity test showed that chitosan was not toxic to Artemia salina Leach, indicated by the LC50 value > 1000µg/ml. chitosan windu 4994.16 g/ml, chitosan is not toxic