<![CDATA[ABSTRACT Background : Hands are a moving medium that is able to move microorganisms from one place to another, from sick people to healthy people, from dirty surfaces to clean surfaces. Therefore washing hands as an effort to maintain hand hygiene is one of the most important actions in preventing the transmission of disease-causing microorganisms both in the environment of everyday life and in preventing high rates of patient morbidity and mortality in health facilities such as hospitals, health centers, and other consequences. nosocomial infections or what is known as Health-care Associated Infections (HAIs). The purpose of this research was to compare the effectiveness of washing hands with seven steps using electrolyzed oxidized water (EOW) and antiseptic soap in maintaining hand hygiene.Methods: This type of research is an analytical study with a pretest-posttest two-group experiment design with random sampling. The research sample was 96 students by swab cotton sticks between their fingers. The cotton swabs were grown in nutrient agar on a petri dish and the colonies count was counted after incubation 16-18 hours in an incubator at 37o Celsius. Data analysis will use statistics with a statistical value of p <0.1.Results: The effectiveness of hands washing with EOW and antiseptic soap can be described as the difference in the number of germ colonies in the seven-step hand washing group with EOW and the difference in the number of germ colonies in the seven-step hand washing group using antiseptic soap. The results of statistical analysis with the Mann Whitney test showed insignificant results p = 0.792. Where the p value <0.1, there is no difference between washing hands with seven steps using EOW with antiseptic soap.Conclusion: There is no difference in the effectiveness of washing hands using EOW with antiseptic]]>
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