Agrotech Journal
Vol 7, No 2 (2022): Agrotech Journal

The Effect of BAP Concentration on In-Vitro Mutant Taro Regeneration

N. Nasaruddin (Universitas Hasanuddin)
Feranita Haring (Universitas Hasanuddin)
Nuriyah Magfiratul Fara Ramadhani (Universitas Hasanuddin)
Salsabila Alisyah (Universitas Hasanuddin)
M. Mukminati (Universitas Hasanuddin)
A.Chamsitasari Zulfikarahmi A. Jamil (Universitas Hasanuddin)
Shelfina Indrayanti (Universitas Hasanuddin)



Article Info

Publish Date
19 Apr 2023

Abstract

Plant regeneration through invitro culture is strongly influenced by the use of growth regulators. BAP is a class of cytokinins that affect cell division which is very well used to stimulate plant growth. The aim of the study was to determine the effect of BAP concentration on the regeneration of various in vitro mutant taro. The study was carried out at the Plant Tissue Culture Laboratory,  Faculty of Agriculture, Hasanuddin University, Makassar. The method used was factorial completely randomized design with 2 factors as the environmental design. The first factor was the BAP concentrations of 0.0 mg/L, 1.0 mg/L, 1.5 mg/L, 2.0 mg/L and 2.5 mg/L. The second factor was mutant plants consisting of control plants (diploid), mutant 1 (Putative-Tetraploid), mutant 2 (Mixoploid), mutant 3 (Mixoploid), and mutant 4 (Putative-Tetraploid). The results showed that the best regeneration was obtained by BAP concentration of 0 mg/L with control plants on the parameters of root length (2.41 cm), number of roots (11.66 pieces) and plantlet formation rate (13 days). The BAP concentration of 1 mg/L was shown by mutant plant 1 on the parameters of the number of shoots (26 pieces) and number of leaves (16 pieces). BAP concentration of 0 mg/L in mutant 4 had a shoot height of 1.83 cm. The results of ploidy flow cytometry analysis of mutant plants with Putative-Tetraploid parental traits after regeneration resulted in detectable diploid chromosomes that were different from the parent types in all BAP concentration treatments 

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Journal Info

Abbrev

ATJ

Publisher

Subject

Agriculture, Biological Sciences & Forestry

Description

The focus and scope of Agrotech Journal cover five disciplines: Plant Disease, Horticulture, Soil Science, Agronomy and plant ...