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All Journal Agrotech Journal
M. Mukminati
Universitas Hasanuddin
Agriculture, Biological Sciences & Forestry

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The Effect of BAP Concentration on In-Vitro Mutant Taro Regeneration N. Nasaruddin; Feranita Haring; Nuriyah Magfiratul Fara Ramadhani; Salsabila Alisyah; M. Mukminati; A.Chamsitasari Zulfikarahmi A. Jamil; Shelfina Indrayanti
Agrotech Journal Vol 7, No 2 (2022): Agrotech Journal
Publisher : Universitas Sembilanbelas November Kolaka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31327/atj.v7i2.1873

Abstract

Plant regeneration through invitro culture is strongly influenced by the use of growth regulators. BAP is a class of cytokinins that affect cell division which is very well used to stimulate plant growth. The aim of the study was to determine the effect of BAP concentration on the regeneration of various in vitro mutant taro. The study was carried out at the Plant Tissue Culture Laboratory,  Faculty of Agriculture, Hasanuddin University, Makassar. The method used was factorial completely randomized design with 2 factors as the environmental design. The first factor was the BAP concentrations of 0.0 mg/L, 1.0 mg/L, 1.5 mg/L, 2.0 mg/L and 2.5 mg/L. The second factor was mutant plants consisting of control plants (diploid), mutant 1 (Putative-Tetraploid), mutant 2 (Mixoploid), mutant 3 (Mixoploid), and mutant 4 (Putative-Tetraploid). The results showed that the best regeneration was obtained by BAP concentration of 0 mg/L with control plants on the parameters of root length (2.41 cm), number of roots (11.66 pieces) and plantlet formation rate (13 days). The BAP concentration of 1 mg/L was shown by mutant plant 1 on the parameters of the number of shoots (26 pieces) and number of leaves (16 pieces). BAP concentration of 0 mg/L in mutant 4 had a shoot height of 1.83 cm. The results of ploidy flow cytometry analysis of mutant plants with Putative-Tetraploid parental traits after regeneration resulted in detectable diploid chromosomes that were different from the parent types in all BAP concentration treatments 
Tolerance of Some Rice Varieties to Drough Based on Drone-Vegetation Index Muh. Farid BDR*; Haris Syaputra Renhard; Nur Qalbi Zaesar Muharram; M. Mukminati; Muh. Fuad Anshori; Nirwansyah Amier; Andi Isti Sakinah
Agrotech Journal Vol 8, No 1 (2023): Agrotech Journal
Publisher : Universitas Sembilanbelas November Kolaka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31327/atj.v8i1.1991

Abstract

The utilization of phenotype technology in breeding is directed to see and select plants based on the appearance of the targeted phenotype. One is modern technology based on geographic information systems and remote sensing. The unmanned aerial drone (UAV) uses computing and machine learning in image processing and information extraction. This research focuses on developing tolerance screening of several varieties to drought based on the drone-vegetation index. This study used nine rice varieties in the rice fields of Kab. Bone-SulSel in the dry season. This research aims to identify the best selection character as a measure of tolerance of rice varieties to drought based on image and morphophysiology, which will be used to develop image-based phenotype-based selection methods. This research was conducted with Randomized Block Design (RAK) consisting of 3 replications. The morpho-physiological parameters of several rice varieties showed a significant effect, including the Inpago 15 variety, which gave the best results on NDVI characters and was followed by other characters. The vegetation index, or the greenness of the vegetation value obtained from digital signal processing of several channels of satellite sensor data, can provide information that a plant has good vegetation. The varieties that gave the best treatment were the Inpago 15 and Jeliteng varieties. The results of the correlation analysis showed that the parameter with a significant positive correlation with productivity was the weight parameter of 1000 grain (r=0.35). 
Co-Authors A.Chamsitasari Zulfikarahmi A. Jamil Andi Isti Sakinah Feranita Haring Haris Syaputra Renhard Muh. Farid BDR* Muh. Fuad Anshori N. Nasaruddin Nirwansyah Amier Nur Qalbi Zaesar Muharram Nuriyah Magfiratul Fara Ramadhani Salsabila Alisyah Shelfina Indrayanti