DETECTION OF SAG1 AND BAG1 Toxoplasma gondii DNA PROBES LABELLED WITH DIGOXIGENIN-11-dUTP

Ida Ayu Pasti Apsari (Faculty of Veterinary Medicine, Udayana University, Denpasar)
Wayan Tunas Artama (Faculty of Veterinary Medicine, Gadjah Mada University, Yogyakarta)
Sumartono s (Faculty of Veterinary Medicine, Gadjah Mada University, Yogyakarta)
I Made Damriyasa (Faculty of Veterinary Medicine, Udayana University, Denpasar)

Jurnal Kedokteran Hewan
Vol 7, No 1 (2013): March

The objective of this research was to detect a minimum concentration of the probes that could be used for dot blot hybridization analysis. The method required labeled DNA probes. In this study a non-radioactive label of Digoxigenin-11-dUTP was used for labeling the Sag1 and the Bag1 of Toxoplasma gondii DNA probe. Labeling method for the probes was done according to the random primed labeling technique. The result showed that 0.67 pg/µl Sag1 probe and 0.58 pg/µl Bag1 probe could be detected by anti-Dig-antibody. It could be concluded that 0.67 pg/µl Sag1 probe and 0.58 pg/µl Bag1 probe could be used to diagnose toxoplasmosis by dot blot hybridization method.