Sumartono s
Faculty of Veterinary Medicine, Gadjah Mada University, Yogyakarta

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A Development of Homolog Sequence of Eimeria tenella Partial Genome as a Probe for Molecular Diagnosis of Coccidiosis S, Sumartono
Indonesian Journal of Biotechnology Vol 10, No 1 (2005)
Publisher : Universitas Gadjah Mada

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Abstract

The goal of the research was to develop a homolog sequence of Eimeria tenella partial genome as a molecularprobe for diagnose coccidiosis using dot blot method. A probe of homolog sequence of E.tenella partial genomeand a non radioactive label, dig-11-dUTP, were used for this research. Four concentrations of molecular probelabeled with dig-11-dUTP, namely, 158,33 pg/μl, 52,25 pg/μl, 15,83 pg/μl and 5,225 pg/μl were tested to detect0,6551 μg DNA target. The procedure of labeling and hybridization detection between DNA target with themolecular probe labeled with dig-11-dUTP were carried out with Digh high prime DNA labeling and detectionstarter Kit I. The conclusion of the research was that 52,25 pg/μl molecular probe or more which its sequenceGGCA CAGTATCCTCCTTCAGGGCAGGG CTCGCACTGGTCAAA CGCGG TAC CATT could detect DNAtarget by dot blot method.Keywords: coccidiosis, E. tenella genome, molecular probe, dot blot hybridization
PELATIHAN DAN PENDAMPINGAN DALAM MENUMBUHKAN RASA PERCAYA DIRI MELALUI LATIHAN DASAR KEPEMIPINAN DAN PUBLIC SPEAKING BAGI PENGURUS OSIS SMAN 3 TAMBUN SELATAN, BEKASI Astuti, Hani; Dewi, Nita Komala; S, Sumartono
Jurnal Terapan Abdimas Vol 8, No 1 (2023)
Publisher : UNIVERSITAS PGRI MADIUN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25273/jta.v8i1.14263

Abstract

Abstract. The purpose of the PKM activity is to provide training and knowledge/insights about growing self-confidence with public speaking techniques and basic leadership training for OSIS management members at SMAN 3 Tambun Selatan, Bekasi. The reason the team provides PKM in the form of training and mentoring to partners, where partners experience several problems, namely many of the OSIS management members do not yet have a leadership spirit, especially in organizing and their self-confidence is still lacking when speaking in public. The offer is to provide training to partners for 2 days with the implementation method, namely the preparation stage, implementation stage, and evaluation stage. The three stages were carried out starting from Wednesday – Friday, June 15 – June 17, 2022 in the SMAN 3 Tambun Selatan hall and attended by 30 participants. The presence of an enthusiastic attitude from the participants is one of the targets of the activity. In addition, the results of the PKM are the increased ability of the participants to speak in public and the increased knowledge of participants about organization. Abstrak. Tujuan kegiatan PKM adalah memberikan pelatihan dan ilmu/wawasan mengenai menumbuhkan kepercayaan diri dengan teknik public speaking dan latihan dasar kepemimpinan bagi anggota pengurus OSIS SMAN 3 Tambun Selatan, Bekasi. Alasan tim memberikan PKM dalam bentuk pelatihan dan pendampingan kepada mitra, dimana mitra mengalami beberapa permsalahan yakni banyak dari anggota pengurus OSIS yang belum memiliki jiwa kepemimpinan khususnya dalam beroganisasi dan rasa percaya diri yang dimiliki masih kurang apabila berbicara di depan umum.Untuk itu, solusi yang ditawarkan yakni memberikan pelatihan kepada mitra selama 2 hari dengan metode pelaksanaan yakni tahapan persiapan, tahapan pelaksanaan, dan tahapan evaluasi. Ketiga tahapan tersebut dilaksanakan mulai dari hari Rabu – Jumat, 15 Juni – 17 Juni 2022 di Aula SMAN 3 Tambun Selatan dan diikuti oleh 30 orang peserta. Adanya sikap antusias dari peserta merupakan salah satu dari target kegaiatan selain itu, hasil dari PKM yakni meningkatnya kemampuan dari peserta untuk berbicara di depan umum dan meningkatnya pengetahuan peserta mengenai keorganisasian.