Cytotoxicity of leaves, stems, and flowers of Kecubung (Datura metel) extracts using the Brine Shrimp Lethality Test (BSLT) method

Rindhira Humairani (Department of Aquaculture, Faculty of Agriculture, Universitas Almuslim, Bireuen 24261, Indonesia.)
Nanda Rizki Purnama (Department of Fisheries Resources Utilization, Faculty of Marine and Fisheries, Universitas Syiah Kuala, Banda Aceh, Indonesia.)
Herpandi Herpandi (Department of Fisheries Products Technology, Faculty of Agriculture, Universitas Sriwijaya, Indralaya Ogan Ilir, South Sumatera 30662, Indonesia.)
Mochamad Syaifudin (Department of Aquaculture, Faculty of Agriculture, Universitas Sriwijaya, Indralaya Ogan Ilir, South Sumatera 30662, Indonesia.)
Ilham Zulfahmi (Department of Fisheries Resources Utilization, Faculty of Marine and Fisheries, Universitas Syiah Kuala, Banda Aceh, Indonesia.)
Yusrizal Akmal (Department of Aquaculture, Faculty of Agriculture, Universitas Almuslim, Bireuen 24261, Indonesia.)
Muliari Muliari (Department of Aquaculture, Faculty of Agriculture, Universitas Almuslim, Bireuen 24261, Indonesia.)
Agus Putra AS (Department of Aquaculture, Faculty of Agriculture, Universitas Samudra, Langsa 24416, Indonesia.)
Agung Setia Batubara (Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Medan, Medan, Indonesia.)

Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan
Vol 12, No 2 (2023): AUGUST 2023

Brine shrimp (Artemia salina) cytotoxicity assays (BSLT) are one of the commonly used cytotoxic test methods to assess a plant extract's pharmacological activity and toxicity. Thus, this study aimed to examine the toxic levels of Kecubung (Datura metel) leaves extracted using different solvents, namely ethanol and ethyl acetate. The results showed that the LC50 value of flower ethanol, flower ethyl acetate, leaf ethanol, and stem ethyl acetate extracts had an LC50 value 1000, which was included in the toxic category. The LC50 value of flower ethanol is 121.044 ppm, flower ethyl acetate 105.89 ppm, leaf ethanol 639.589 ppm, and stem ethyl acetate 635.276 ppm. Ethanol leaf extract at a concentration of 1000 ppm showed the highest mortality with a percentage of 60% of the total number of A. salina. The flower ethanol extract showed the highest mortality at a concentration of 250 ppm with a percentage of 67% and at a concentration of 1000 ppm with a percentage of 70% of the total number of A. salina. Meanwhile, flower ethyl acetate and stem ethyl acetate extract at a concentration of 1000 ppm obtained 100% mortality of A. salina in the first 6 hours. The result shows that the flower ethyl acetate and stem ethyl acetate extract at a concentration of 1000 ppm is very toxic compared to other concentrations.Keywords:ToxicityArtemia salinaLC50Ethyl acetateEthanol