Jurnal Pembelajaran dan Biologi Nukleus
Vol 9, No 3 (2023): Jurnal Pembelajaran Dan Biologi Nukleus November 2023

Optimation of Green Synthesis Biopolymer Cellulose Using Acetobacter xylinum From Whey as Media of Bacteria

Suharni Sinaga (Department of Midwifery, Sekolah Tinggi Ilmu Kesehatan (STIKes) Senior Medan)
Junius Gian Ginting (Deparment of Pharmacy, Sekolah Tinggi Ilmu Kesehatan (STIKes) Senior Medan)
Helen Anjelina Simanjuntak (Department of Pharmacy, Sekolah Tinggi Ilmu Kesehatan (STIKes) Senior Medan)
Dame Meldaria sipahutar (Department of Radiodiagnostics and radiotherapy, Sekolah Tinggi Ilmu Kesehatan (STIKes) Senior Medan)
Defacto Firmawai Zega (Department of Midwifery, Sekolah Tinggi Ilmu Kesehatan (STIKes) Senior Medan)
Yanti Yanti (Department of Midwifery Profession)
Sahat Sinaga (Department of Radiodiagnostics and radiotherapy, Sekolah Tinggi Ilmu Kesehatan (STIKes) Senior Medan)
Lydia Barus (Department of Midwifery, Sekolah Tinggi Ilmu Kesehatan (STIKes) Senior Medan)
Hermawan Purba (Department of Pharmacy, Sekolah Tinggi Ilmu Kesehatan (STIKes) Senior Medan)



Article Info

Publish Date
24 Nov 2023

Abstract

Utilizing waste into a more valuable commodity was one of the objectives of that research. Waste which initially became an environmental pollutant factor could be processed into more useful products. Tofu liquid waste was referred to whey was a by-product of tofu production. Whey was waste that still contains organic materials such as protein, fat, carbohydrates and minerals. That whey was used as a growth medium for Acetobacter xylinum bacteria and produces cellulose biopolymer as a result of carbohydrate fermentation. The process of optimizing carbohydrate fermentation by Acetobacter xylinum bacteria was carried out by varying the ratio of the amount of whey to coconut water, the acidity level of the media, and the fermentation time. The research results showed that the biosynthesis of cellulose biopolymer was obtained from a ratio of whey concentration to coconut water of 1:1, variations in acidity levels showed that the optimum fermentation conditions occurred at pH 4, and the optimal amount of cellulose biopolymer was obtained on day 21, namely with the highest percent yield value. The resulting biocellulose was characterized using FTIR, XRD, and SEM. The characterization results show that biocellulose is well formed with a fiber surface shape and particle size in the range of 50-200 nm

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Journal Info

Abbrev

nukleus

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media

Description

Jurnal Pendidikan Biologi merupakan jurnal elektronik yang merupakan wadah penerbitan artikel penelitian original yang terkait dengan penelitian pendidikan biologi. Jurnal ini dikelola oleh Program Studi Pendidikan Biologidibawah naungan LPPM Universitas Labuhanbatu. Jurnal ini diterbitkan dua kali ...