α-glucosidase Inhibitory Activity of Probiotic Isolate LBSU9 Isolated from Traditional Food “Trites”: a Preliminary Study

Edy Fachrial (Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Riau, Indonesia)
Ismawati Ismawati (Department of Biochemistry, Faculty of Medicine, Universitas Riau, Indonesia)
Titania T Nugroho (Faculty of Mathematics and Natural Science, Universitas Riau, Indonesia)
Saryono Saryono (Faculty of Mathematics and Natural Science, Universitas Riau, Indonesia)

International Journal of Basic and Applied Science
Vol. 12 No. 4 (2024): March: Basic and Applied Science

As the prevalence of type 2 diabetes mellitus continues to increase over the years, research into antidiabetic drugs needs to continue. Probiotics are non-pathogenic microorganisms that have potential as α-glucosidase inhibitors. This study aimed to characterize and determine the α-glucosidase inhibitor activity of LBSU9 isolate, a probiotic isolated from "trites," a traditional food of North Sumatra. The results showed that LBSU9 had bacillus morphology, Gram positive, negative catalase test, TSIA (Triple Sugar Iron Agar) A/A test and non-hemolysis. LBSU9 had good tolerance to simulated gastric acid and bile salts, with growth percentages of 66.54% and 64.74%. LBSU9 also has potent antimicrobial activity against S.aureus and E.coli, with 20 mm and 17 mm inhibition zones, respectively. The alpha-glucosidase inhibitor activity of LBSU9 was 98.4% greater than that of acarbose, which was 97%. Based on the results of this study, it is concluded that LBSU9 isolate has the potential to be a complementary therapy to prevent or treat type 2 diabetes mellitus