Synthesis and Toxicity Tests of N-Carbothioamide-3-(2,4- Dichlorophenyl)-5-(4-Hydroxy-3-Methoxyphenyl)Pyrazoline

Muhammad Irfan (Laboratorium Penelitian dan Pengembangan Kefarmasian "Farmaka Tropis", Fakultas Farmasi, Universitas Mulawarman, Samarinda, Kalimantan Timur, Indonesia)
Lisna Meylina (Laboratorium Penelitian dan Pengembangan Kefarmasian "Farmaka Tropis", Fakultas Farmasi, Universitas Mulawarman, Samarinda, Kalimantan Timur, Indonesia)
Agung Ramadhani (Program Studi Pendidikan Kimia, Fakultas Keguruan dan Ilmu Pendidikan, Universitas Mulawarman, Samarinda, Indonesia)
Rolan Rusli (Laboratorium Penelitian dan Pengembangan Kefarmasian "Farmaka Tropis", Fakultas Farmasi, Universitas Mulawarman, Samarinda, Kalimantan Timur, Indonesia)

Journal of Tropical Pharmacy and Chemistry
Vol. 8 No. 1 (2024): J. Trop. Pharm. Chem.

Pyrazoline is an alkaloid compound which has various biological activities such as antibacterial, antifungal, antitumor and anticancer. The compound N-carbotioamide-3-(2,4-dichlorophenyl)-5-(4-hydroxy-3-methoxyphenyl)pyrazoline was successfully synthesized from the basic ingredients 2',4'-dichloro-4-hydroxy-3-methoxychalcone and thiosemicarbazide with sodium hydroxide catalyst at 80°C for 7 hours. The synthesized compound was characterized using 1H-NMR, 13C-NMR, and mass spectroscopy and had a yield of 28.03%. Based on the results of toxicity tests using the Brine Shrimp Lethality Test (BSLT) method, this compound has an LC50 value of 44.6 ppm and has the potential to be an antimicrobial compound.