Massive conversion of agricultural land, especially on wetlands, has resulted in declining rice productivity, so extending cultivation to drylands is considered appropriate. However, limited water availability is a major constraint that cannot be ruled out, so the procurement of drought-adaptive high-yielding varieties is considered to be the most effective solution. The purpose of this study was to evaluate drought-tolerant mutant genotypes based on proline content and yield component characters at various percentages of water availability. The experiment was arranged using a factorial complete randomized design, the first factor is drought stress consisting of 100% field capacity (K3), 66% (K2), 33% (K1) and the second factor is genotype consisting of inpago unram (P1), MD200-G13-3-11-5 (P2), MD300-G20-8-3-5 (P3), MD200-G24-17-10-8 (P4), MD300-G27-16-9-5 (P5). The observed characters consisted of proline content, flag leaf length, panicle length, filled grain weight and hollow grain weight. The results showed that MD300-G27-16-9-5 (P5) is a mutant plant that has the greatest potential to obtain drought-tolerant traits, although the level of proline produced is not as high as Inpago Unram (P1) and MD200-G24-17-10-8 (P4), but the consistency of adaptation shown by MD300-G27-16-9-5 (P5) when experiencing drought stress tends not to cause a significant decrease in all yield component characters, namely flag leaf length, panicle length, filled grain weight and unfilled grain weight
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