Meta: Journal of Science and Technological Education
Vol. 3 No. 2 (2024)

Colony Growth Rate and Morphological Characterization of Bovine Rumen Bacteria in Fermented Liquid

Triono Bambang Irawan (Politeknik Negeri Jember)
Indra Satria Kusuma (Politeknik Negeri Jember)
Liliek Dwi Soelaksini (Politeknik Negeri Jember)
Anni Nuraisyah (Politeknik Negeri Jember)



Article Info

Publish Date
26 Dec 2024

Abstract

Bovine rumen bacteria consist of diverse microorganisms essential for processing manure, compost, and liquid organic fertilizer, enhancing soil fertility and nutrient availability while suppressing pests and pathogens. These bacteria include cellulose-digesting species such as Bacteroides succinogenes, Ruminococcus flavefaciens, and Ruminococcus albus, which produce cellulase enzymes for organic matter decomposition. Morphologically, cellulolytic bacteria are categorized into coccus forms (Ruminococcus flavefaciens and Ruminococcus albus) and rod forms (Bacteroides succinogenes and Butyrivibrio fibrisolvens), with Gram-positive and Gram-negative cell walls. This study examined the growth and morphology of bovine rumen bacteria in fermented liquid over three weeks. Colony counts increased from 4.75 × 10⁶ CFU/mL in week 1 to 2.92 × 10⁷ CFU/mL in week 2 and 6.2 × 10⁸ CFU/mL in week 3. Colony morphologies included colors (pink, yellow, blue center), sizes (small, medium, large), shapes (circular, irregular), elevations (raised, convex, unbonated), surfaces (smooth, mucoid), and edges (regular, irregular). Predominant bacterial cell shapes were bacillus, with Gram-positive staining. These findings underscore the potential of bovine rumen bacteria in organic waste fermentation and nutrient cycling.

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Journal Info

Abbrev

meta

Publisher

Subject

Education Social Sciences Other

Description

META: Journal of Science and Technological Education accepts research papers on Science and Technological Education. As a result, its goal is to connect practice and research by giving information, views, opinions, and insights. META: Journal of Science and Technological Education, as a global ...