This research aims to develop a digital-based practicum guide to stimulate the critical thinking skills of high school students on cell structure materials. This research uses the 4D model development method (Define, Design, Develop, Disseminate), which consists of the stages of defining needs, product design, development, and dissemination. The product is developed in the form of an interactive flipbook that contains videos, images, critical thinking simulation questions, and answer links. The validation results showed that the digital-based practicum guide was declared very valid by media experts with a score of 97 and by material experts with a score of 95. The practicality test involving 74 students showed an average score of 90 in the "very practical" category. This guide is considered effective because it facilitates self-paced learning, is easily accessible, and increases student engagement through interactive features. The discussion of the results shows that this guide can support the student learning process, increase motivation, and provide an interesting and meaningful learning experience. This study concludes that the digital-based practicum guide that was developed is valid, practical, and feasible to be used to support biology learning that trains critical thinking skills.
                        
                        
                        
                        
                            
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