MEDICA (International Medical Scientific Journal)
Vol. 6 No. 3 (2024): MEDICA (Internasional Medical Scientific Journal)

The Antibacterial Effectiveness of Moringa Oleifera Leaves Extract Against the Growth of Escherichia coli Using the Diffusion Method

Annisa, Liza Harum Nur (Unknown)
Triana, Linda (Unknown)
Sari, Emilda (Unknown)
Nuswantoro, Ari (Unknown)



Article Info

Publish Date
30 Oct 2024

Abstract

Moringa oleifera leaves a common herbal plant found in tropical regions and naturalized in Indonesia, are known to be rich in bioactive compounds such as flavonoids, tannins, terpenoids, alkaloids, and saponins. These compounds have potential medicinal properties, including antibacterial activity. This study aims to examine the antibacterial effectiveness of moringa leaf extract against the growth of Escherichia coli using the diffusion method. This descriptive-analytical research employed a purposive sampling technique with varying concentrations of moringa leaf extract: 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, and 100%. Each concentration was tested in triplicate, resulting in a total of 30 samples. The antibacterial effectiveness test was conducted using the agar diffusion method with blank paper disks. The results showed that no inhibition zones were formed at concentrations of 10% and 20%. Inhibition zones began to appear at 30% concentration (7.3 mm), increasing with higher concentrations: 40% (9 mm), 50% (10.3 mm), 60% (11.3 mm), 70% (16.6 mm), 80% (23.3 mm), 90% (28.3 mm), and reaching 34.6 mm at 100% concentration. Based on the antibacterial activity category, 10% and 20% showed no activity, 30% and 40% were categorized as weak, 50% and 60% as moderate, 70% as strong, and 80%, 90%, and 100% as very strong. It can be concluded that an 80% concentration of moringa leaf extract is the most effective in inhibiting the growth of Escherichia coli.

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Journal Info

Abbrev

medica

Publisher

Subject

Chemistry Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Veterinary

Description

MEDICA (International Medical Scientific Journal), with registered number ISSN 2622-6596 (Print), 2622-660X (Online) is an Open Access and peer-reviewed journal presenting timely research on all aspects of health that other media have not published. Managed by the Borneo Scientific Publishing, ...