Jurnal Info Kesehatan
Vol 19 No 2 (2021): JURNAL INFO KESEHATAN

The Effectiveness of Chocolate in Reducing the Number of Methicillin-Resistant Staphylococcus aureus Colonies in Rattus norvegicus

Edy Suwandi (Department of Medical Laboratory Technology, Poltekkes Kemenkes Pontianak, Pontianak, West Kalimantan, Indonesia.)
Ari Nuswantoro (Department of Medical Laboratory Technology, Poltekkes Kemenkes Pontianak, Pontianak, West Kalimantan, Indonesia.)
Sugito Sugito (Department of Medical Laboratory Technology, Poltekkes Kemenkes Pontianak, Pontianak, West Kalimantan, Indonesia.)
Desi Wahyumarniasari (Department of Medical Laboratory Technology, Poltekkes Kemenkes Pontianak, Pontianak, West Kalimantan, Indonesia.)
Muhammad Reza Setiawan (Department of Medical Laboratory Technology, Poltekkes Kemenkes Pontianak, Pontianak, West Kalimantan, Indonesia.)
Dinasti Aprillia (Unit Pelaksana Teknis Pusat Laboratorium Kesehatan Kota Pontianak, Pontianak, West Kalimantan, Indonesia.)
Devi Nurfitri Bintang (Department of Pharmacy, Akademi Farmasi Yarsi Pontianak, Pontianak, West Kalimantan, Indonesia.)



Article Info

Publish Date
31 Dec 2021

Abstract

Chocolate has long been understood to provide positive emotions and a good mood if consumed in moderation. Chocolate contains prebiotics naturally from its constituent ingredients produced during the production process. Prebiotics, frequently oligosaccharides, are substances which cannot be metabolized by the human digestive system but can be employed by a group of bacteria in the gut, understood as probiotics. The positive relationship among them provides benefits for the host in eliminating pathogens. One of the well-known pathogens which frequently cause infection either in the community or in hospitals is methicillin-resistant Staphylococcus aureus (MRSA). Since it was first identified in 1960, MRSA has caused health problems until today. Research conducted on two groups of Rattus norvegicus infected with MRSA and then fed chocolate revealed a decrease in the average number of bacterial colonies on the skin compared to the control group. In the group fed chocolate at a dose of 50 mg/day, the bacterial colonies decreased to 1.28 x 108 CFU/cm2 in 7 days, lower than in the control group (1.46 x 108 CFU/cm2) at the same time. While those fed 75 mg/day chocolate decreased to 2.70 x 107 CFU/cm2 and the three groups were significantly different (0.000<0.05). Prebiotics fermented by probiotics release short-chain fatty acids (SCFA), which compete with the pathogens for attaching to the epithelial wall so that pathogens lose space and nutrients to survive. However, the adverse effect of chocolate may occur because it contains sugar which is a nutrient for bacteria, but if the balance of normal flora and adequate intake of prebiotics are administered, the pathogen could be eliminated.

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Journal Info

Abbrev

infokes

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Health Professions Immunology & microbiology

Description

Jurnal Info Kesehatan with registered number ISSN 0216-504X (Print), 2620-536X (Online) is a scientific journal which managed by the Research and Community Service Unit, Politeknik Kesehatan Kemenkes Kupang in cooperation with Indonesian Oral Health and Therapists Association with the MoU. The ...