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All Journal Meditory : The Journal of Medical Laboratory Jurnal Kesehatan Jurnal teknologi Kesehatan Borneo Jakiyah : Jurnal Ilmiah Umum dan Kesehatan Aisyiyah Jurnal Laboratorium Khatulistiwa Jurnal Info Kesehatan
Suwandi, Edy
Department Of Medical Laboratory Technology, Poltekkes Kemenkes Pontianak
Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Education Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Veterinary

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Combination of Aloe Vera and Chocolate to Reduce The Number of The Colony of Staphylococcus Aureus Edy Suwandi; Ari Nuswantoro; Sugito Sugito
Jurnal teknologi Kesehatan Borneo Vol 3 No 2 (2022): Jurnal teknologi Kesehatan Borneo
Publisher : POLTEKKES KEMENKES PONTIANAK

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jtkb.v3i2.197

Abstract

Aloe vera (AV) and chocolate are good examples of functional foods because they contain prebiotics that can help the host defend itself against harmful bacterial infections such as Staphylococcus aureus. Many studies have used AV or chocolate alone to help fight infection, but not much is known about using both together. This study aims to count the number of colonies of S. aureus infected on mice’s skin (Mus musculus) after being given AV and cocoa in certain doses per kilogram of body weight. A total of 35 healthy male mice were infected with S. aureus in the superficial area of ​​the nape of the skin. After that, they were divided into seven groups, each consisting of 5 mice, and given the following treatments: given 50 mg/kg BW AV (group A), 50 mg/kg BW brown (B), 50 mg/kg BW combination of AV and chocolate (C), given 75 mg/kg BW AV (D), given 75 mg/kg BW brown (E), given 75 mg/kg BW combination of AV and chocolate (F), and control (without prebiotic administration), K). Mice that were given 75 mg/kg BW AV/chocolate/AV+chocolate had a better ability to reduce the number of bacteria than those that were given 50 mg/kg BW AV/chocolate/AV+chocolate. In addition, rats that were given AV and chocolate together had a better ability to reduce the number of bacteria than those that were given either only AV or chocolate alone. Prebiotics are both found in AV and chocolate but with different substances. This causes mice given a combination of prebiotics to be considered better than those given only one type of prebiotic source.
AKTIVITAS ANTI BAKTERI FORMULASI LOTION EKSTRAK ETANOL RIMPANG JERINGAU PUTIH Emilda Sari; Linda Triana; Sugito Sugito; Edy Suwandi
Meditory : The Journal of Medical Laboratory Vol 11, No 1 (2023): Meditory, Volume 11 No. 1 Tahun 2023
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v11i1.2292

Abstract

Background: The skin is the most important and largest organ of the human body. The skin needs so much attention, both in terms of disease and health,because it turns out that the skin itself is quite susceptible to bacterial and fungal attacks. Therefore, the formulation of lotions for the skin, namely ingredients that can kill bacteria or fungi and are safe for the skin can be justified. Natural ingredients that have properties as antibacterial ingredients for the skin are White Jeringau. Aims:The purpose of this study was to explain the differences in the inhibition of the lotion formulation of the ethanol extract of the White Jeringau Rhizome against the growth of Staphylococcus aureus bacteria using the diffusion method. Methods:The research method used is a quasi-experimental with purposive sampling technique. The sample in this study was the lotion of the ethanol extract of the Jeringau Putih rhizome with concentrations of 40%, 50% and 60% which were replicated 9 times each so that the number of samples in this study were 27 samples. Furthermore, the sample was tested for inhibition of Staphylococcus aureus bacteria by diffusion method. Results:Based on the results of this study in 2022, antibacterial analysis of the white jeringau extract lotion sample formula I, formula II and formula III against Staphylococcus aureus bacteria using the Kirby-bauer diffusion method showed the results of the inhibition zone diameter, namely in formula I the average inhibition zone formed was 11 .50 mm, in formula II it is 13.61 mm and in formula III it is 14.50 mm. According to Davis and Stout (1971) the inhibitory response by active ingredients is grouped into 4categories, namely weak activity (? 5 mm), moderate (5-10 mm), strong (10-20 mm), and very strong (? 20 mm). Conclusion:Based on this classification, white jeringau extract lotion formula I, formula II and formula III are categorized as strong. Formula Ihad the best inhibitory response because the concentration of the extract was the smallest and had a strong category of inhibition compared to formula II and formula III which had a higher concentration and had the same inhibitory power. the results of statistical test analysis using the Kruskal Walis test obtained p value = 0.000 0.05 and it was concluded that there were differences in the white jeringau rhizome lotion formula I, II, and III in inhibiting the growth of Staphylococcus aureus bacteria.
ANALISIS DAYA HAMBAT FORMULA ANTISEPTIK GEL PEMBERSIH TANGAN DAUN MANGROVE TERHADAP PERTUMBUHAN BAKTERI Staphylococcus aureus edy suwandi; Kamarudin; Ratih Indrawati; Emilda Sari; Sugito
Jurnal Ilmiah Umum dan Kesehatan Aisyiyah Vol. 8 No. 1 (2023): JAKIYAH VOL. 8 NO. 1 JUNI 2023
Publisher : Program Studi Kebidanan Politeknik Aisyiyah Pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Latar Belakang:  Penggunaan antiseptik tangan diperlukan sebagai salah satu upaya dalam menjaga kebersihan tangan. Namun antiseptik tangan yang beredar dalam pasaran terbuat dari bahan utama alkohol dengan konsentrasi ± 50% sampai 70%. Kandungan alkohol pada hand sanitizer apabila digunakan secara terus menerus dapat menimbulkan rasa terbakar, kulit kering, iritasi, dan tidak dapat digunakan pada kulit luka. Oleh karena itu, diperlukan antiseptik tangan berbahan dasar dari bahan alam yang mempunyai aktivitas daya hambat terhadap bakteri dan aman apabila diaplikasikan pada telapak tangan secara berulang. Salah satu bahan alam yang dapat bersifat sebagai antibakteri adalah daun Mangrove. Daun Mangrove mengandung senyawa bioaktif antibakteri jenis alkaloid, saponin, tanin, fenolik, flavonoid, triterpenoid dan glikosida yang dapat menghambat pertumbuhan bakteri Staphylococcus aureus Tujuan: Penelitian bertujuan menjelaskan perbedaan daya hambat antara formula 1, formula 2 dan formula 3 antiseptik gel pembersih tangan daun Mangrove terhadap pertumbuhan bakteri Staphylococcus aureus. Metode: Desain penelitian yang digunakan yaitu quazi experiment. Pada penelitian menggunakan sampel antiseptik gel pembersih tangan daun Mangrove formula 1, formula 2 dan formula 3 dengan sepuluh kali replikasi setiap perlakuan. Untuk uji daya hambat terhadap bakteri Staphylococcus aureus menggunakan metode difusi Kirby Bauer. Hasil penelitian: Berdasarkan hasil pengujian laboratorium, zona hambat yang terbentuk pada formula 1 rata-rata 9,90 mm, formula 2 rata-rata 12,80 mm dan formula 3 rata-rata 16,00 mm. Hasil analisis statistik menggunakan uji Friedman didapatkan p value = 0,000 < a 0,05. Simpulan: Terdapat perbedaan daya hambat antiseptik gel pembersih tangan daun Mangrove formula 1, formula 2 dan formula 3  terhadap pertumbuhan bakteri Staphylococcus aureus.
Efektifitas Ekstrak Ethanol Daun Pepaya (Carica Papaya L) terhadap Pertumbuhan Bakteri Escherichia Coli dengan Metode Difusi Sugito Sugito; Edy Suwandi
Jurnal Laboratorium Khatulistiwa Vol 1, No 1 (2017): November 2017
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v1i1.91

Abstract

Abstract: Papaya plant (Carica papaya L) is also called medicinal plant which is used in traditional medicine. Part of papaya plants (Carica papaya L) which often utilized are papaya leaf (Carica papaya L) because its content of substances or active ingredients is antibacterial, cancer prevention, increase appetite, and treat some diseases caused by bacteria. The aim of this research was to determined the extracted ethanol of papaya leaf (Carica Papaya L) with the growth of Escherichia coli bacteria by the diffusion method. Research design which used in this study was experimental research, with the method of sampling purposive sampling, then the results analyzed with Friedman Test. A result of research from 5 replications test of extract concentration of papaya leaf by diffusion method obtained that the minimum average concentration was 10,00 and maximize was 14,20. While the minimum inhibitory zone diameter was 10 mm with the concentration of 10% and the maximum was 15 mm with the concentration of 20%. A result of Friedman test, it was found that (p = 0,00 <0,05) so it can be declared that there was a difference of papaya leaf extract (Carica Papaya L) effect on Escherichia coli bacteria growth by the diffusion method.Abstrak: Tanaman pepaya (Carica papaya L) disebut juga tanaman obat yang bermanfaat untuk pengobatan tradisional. Bagian yang sering dimanfaatkan pada tanaman pepaya (Carica papaya L) adalah daun pepaya (Carica papaya L) karena mengandung zat atau bahan aktif yang bersifat sebagai antibakteri, pencegahan kanker, menambah nafsu makan dan mengobati beberapa penyakit yang disebabkan oleb bakteri. Tujuan penelitian adalah untuk mengetahui ekstrak ethanol daun pepaya (Carica Papaya L) terhadap pertumbuhan bakteri Escherichia coli dengan metode difusi. Desain penelitian yang digunakan dalam penelitian ini adalah penelitian eksperimental dengan metode pengambilan sampel purposive sampling, selanjutnya hasil dianalisis menggunakan uji Friedman. Hasil penelitian dari 5 penggulangan uji konsentrasi ekstrak daun pepaya dengan metode difusi dapat diketahui rata-rata konsentrasi minimum 10,00 dan maksimun 14,20. Sedangkan diameter zona hambat minimum adalah 10 mm dengan konsentrasi 10% dan maksimun adalah 15 mm dengan konsentrasi 20%. Hasil Friedmen test diperoleh nilai (p = 0,00 < 0,05) sehingga dapat dinyatakan bahwa terdapat perbedaan efektiftas ekstrak daun pepaya (Carica Papaya L) terhadap pertumbuhan bakteri Escherichia coli dengan metode difusi.
Perbedaan Presisi Pemipetan Sampel Menggunakan Pipet Sahli dan Mikropipet pada Pemeriksaan Hemoglobin Metode Cyanmethemoglobin Hidayatussalihin Hidayatussalihin; Etiek Nurhayati; Edy Suwandi
Jurnal Laboratorium Khatulistiwa Vol 2, No 1 (2018): November 2018
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v2i1.322

Abstract

Abstract: International Commite for Standarization in Hematology (ICSH) recommends hemoglobin examination using Cyanmethemoglobin method. Examination of Hb Cyanmethemoglobin using sahli pipette in sampling. The Sampling method flow through the hose. The use of glass pipets do not have high accuracy and precission for less than volumes 1 ml, so in small fluid displacement less than 1000 (microliter) using micropipet or commonly known as automatic pipette. The aim of this research was to know the precision value between sampling using the sahli pipette and mikropipet on examination of hemoglobin value of Cyanmethemoglobin method accepted or not on standard Clinical Laboratoty Improvement Amendments (CLIA) and to analyze the difference of precision between sampling using pipette sahli and micropipette on examination of hemoglobin Cyanmethemoglobin method. The design of this study was descriptive anlytic. This research using EDTA blood examination with the number of 36 test samples. Preliminary period expression used 60 test samples. The hemoglobin examination using Cyanmethemoglobin method by spectrophotometry. Based on the results of the research, the value of % CV of sahli’s pipet is 4.60% and the value % CV of micropipet is 2.02%. and the value %CV The maximum recommended by the CLIA can be received from the sahli and mikropipet pipette that is <7%. he data obtained were statistically analyze by using Willcoxon Test and the result P 0.000 <0.05. The conclution of this research was found a signifcant difference between Sahli pipette and micropipette.Abstrak: International Commette for Standarization in Hematology (ICSH) merekomendasikan pemeriksaan hemoglobin dengan menggunakan metode Cyanmethemoglobin. Pemeriksaan Hb Cyanmethemoglobin menggunakan pipet sahli dalam pengambilan sampel. Pengambilan sampel dengan cara dihisap melalui selang. Penggunaan pipet gelas tidak mempunyai akurasi dan presisi yang tinggi untuk volume kurang dari 1 ml, sehingga pemindahan cairan dengan volume kecil kurang dari 1000 (microliter) orang cenderung menggunakan mikropipet atau biasa juga dikenal dengan pipet otomatis. Penelitian ini bertujuan untuk mengetahui nilai presisi antara pemipetan sampel menggunakan pipet sahli dan mikropipet pada pemeriksaan kadar hemoglobin metode Cyanmethemoglobin diterima atau tidak pada standar Standar Clinical Laboratoty Improvement Amendments (CLIA) dan menganalisis perbedaan presisi antara pemipetan sampel menggunakan pipet sahli dengan pemipetan sampel menggunakan mikropipet pada pemeriksaan kadar hemoglobin metode Cyanmethemoglobin. Desain penelitian ini adalah deskriptif analitik. Penelitian ini menggunakan bahan pemeriksaan berupa darah EDTA dengan jumlah 36 sampel uji. Ditahapan periode pendahuluan digunakan sebanyak 60 sampel uji. Metode pemeriksaan hemoglobin menggunakan metode Cyanmethemoglobin secara fotometri. Berdasarkan hasil penelitian yang dilakukan diperoleh nilai %CV pipet sahli sebesar 4,60% dan nilai %CV mikropipet sebesar 2,02%. Nilai %CVmaksismum yang di rekomendasikan oleh CLIA dapat diterima dari pipet sahli dan mikropipet yaitu <7%. Data yang diperoleh diolah secara statistik menggunakan uji Willcoxon didapatkan hasil P 0.000<0.05. Sehingga dapat disimpulkan ada perbedaan signifkan antara pipet sahli dan mikropipet.
Toksisitas Akut Ektrak N-Heksan Dan Methanol Rimpang Jeringau Merah Terhadap Kematian Artemia Salina Leach Dengan Metode Brine Shrimp Lethality Test Edy Suwandi; Supriyanto Supriyanto
Jurnal Laboratorium Khatulistiwa Vol 1, No 2 (2018): Mei 2018
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v1i2.149

Abstract

Abstract: Acorus sp (red sweet flag) is an endemic plant from West Borneo which contains chemical substances such as flavonoids that have bioactivity as antioxidants so it is recommended one to prevent cancer. The study aimed to determine toxicity of n-Hexan extract and methanol red sweet flag rhizomes against Artemia salina Leach with Brine Shrimp Lethality Test method. This study was a type of quasi experimental design, sampling technique obtained by using purposive sampling. According to the study results, it can be seen than methanol extract from red sweet flag rhizomes give impact on the mortality average of Artemia salina Leach : concentration 100 µg/ml (51%), 250 µg/ml (75%), 500 µg/ml (92%), 750 µg/ml (96%) and 1000 µg/ml (99%). In addition, n-Hexan extract of red sweet flag rhizomes in 100 µg/ml (45%), 250 µg/ml (60%), 500 µg/ml (92%), 750 µg/ml (84%) and 1000 µg/ml (96%). It is reported based on probit analysis that LC50 of n-Hexan extract from red sweet flag rhizomes was 128.2921 µg/ml meanwhile methanol extract was 242.7169 µg/ml µg/ ml, thus in conclusion n-Hexan and methanol extract of red sweet flag rhizomes potentially cytotoxic to Artemia salina Leach larvae.Abstrak: Jeringau merah (Acorus sp) merupakan satu jenis tanaman endemik Kalimantan Barat memiliki kandungan kimia diantaranya flavonoidyang mempunyai bioaktivitas sebagai antioksidan sehingga sangat baik untuk pencegahan kanker. Penelitian ini bertujuan mengetahui mengetahui dan menentukan toksisitas ekstrak n-Heksan dan Methanol Rimpang Jeringau MerahterhadapArtemia salinaLeachdengan metode Brine Shrimp Lethality Test. Desain penelitian ini adalah eksperimental semu, teknik pengambilan sampel dengan cara purposive sampling. Berdasarkan hasil penelitian diketahui ekstrak Methanol Rimpang Jeringau Merah rerata kematianArtemia salina Leach konsentrasi 100 µg/ml (51%), 250 µg/ ml (75%), 500 µg/ml (92%), 750 µg/ml (96%) dan 1000 µg/ml (99%)pada ekstrak n-Heksan Rimpang Jeringau Merah konsentrasi 100 µg/ml (45%), 250 µg/ml (60%), 500 µg/ml (92%), 750 µg/ml (84%) dan 1000 µg/ml (96%). Dari analisis probit diketahuinilai LC50 ekstrak n-Heksan Rimpang Jeringau Merah yaitu 128.2921 µg/ml dan ekstrak Methanol Rimpang Jeringau Merah yaitu 242.7169 µg/ml µg/ml, maka disimpulkan ekstrak n-Heksan dan Methanol Rimpang Jeringau Merahbersifat sitotoksik terhadap larva Artemia salina Leach.
Pengaruh Air Rebusan Rimpang Jeringau Merah (Acorus Calamus L.) Konsentrasi 100%, 75%, 50%, Dan 25% terhadap Sensitifitas Bakteri Escherichia Coli, Salmonella Typhi dan Shigella sp Edy Suwandi
Jurnal Laboratorium Khatulistiwa Vol 2, No 2 (2019): Mei 2019
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v2i2.327

Abstract

Abstract: Red Jeringau (Acorus calamus L.) is one of the endemic plants of West Kalimantan which contains antibacterial, phytochemical, and antioxidant activity. This plant has been used for generations by people who live in the interior and away from health services as a mixture of traditional medicines. This study aims to determine the effect of the concentration of Jeringau Red rhizome water on the sensitivity of Escherichia coli, Salmonella typhi and Shigella sp with concentrations of 100%, 75%, 50%, and 25%. The Red Jeringau Rhizome is obtained from the Landak Regency and then boiled using distilled water. This study uses a quasi-experimental design with sampling techniques using purposive sampling. This study was conducted with 6 replications for each treatment. The effectiveness test of the Red Jeringau rhizome boiled water was carried out using the Kirby Bauer method with bacterial suspension which was adjusted to Mc Farland’s turbidity standard. The data obtained were then analyzed using a simple linear regression test and obtained p (0.00) <α (0.05) against the bacteria Escherichia coli, Salmonella typhi, and Shigella sp. This means that H1 is accepted so that it can be stated that there is an influence of the concentration of Jeringau Red rhizome water on Escherichia coli, Salmonella typhi, and Shigella sp. The magnitude of the contribution effect of Jeringau Red rhizome on bacterial sensitivity is 85.7% (R = 0.857) in Escherichia coli, 91.1% (R = 0.911) in Salmonella typhi, and 85.7% (R = 0.857) in Shigella sp.Abstrak Jeringau Merah (Acorus calamus L.) merupakan salah satu tanaman endemik Kalimantan Barat yang mengandung antibakteri, ftokimia, dan aktivitas antioksidan. Tumbuhan ini secara turun temurun dimanfaatkan masyarakat yang tinggal dipedalaman dan jauh dari pelayanan kesehatan sebagai ramuan obat tradisional.Penelitian ini bertujuan mengetahui pengaruh konsentrasi air rebusan rimpang Jeringau Merah terhadap sensitiftas bakteri Escherichia coli, Salmonella typi dan Shigella sp dengan konsentrasi 100%, 75%, 50% dan 25%. Rimpang Jeringau Merah diperoleh dari Kabupaten Landak kemudian direbus menggunakan aquadest. Penelitian ini menggunakan desain eksperimen semu (Quasi Experiment) dengan teknik pengmbilan sampel dengan cara purposive sampling. Penelitian ini dilakukan dengan 6 replikasi untuk masing-masing perlakuan. Uji efektiftas air rebusan rimpang Jeringau Merah dilakukan menggunakan metode Kirby Bauer dengan suspensi bakteri yang disesuaikan dengan standar kekeruhan Mc Farland. Data yang diperoleh kemudian dianalisis menggunakan uji regresi linear sederhana dan didapatkan p (0,00) < α (0,05) terhadap bakteri Escherichia coli, Salmonella typhi, dan Shigella sp. Hal ini berarti H1 diterima sehingga dapat dinyatakan bahwa terdapat pengaruh konsentrasi air rebusan rimpang Jeringau Merah terhadap bakteri Escherichia coli, Salmonella typhi, dan Shigella sp. Besarnya kontribusi pengaruh rebusan rimpang Jeringau Merah terhadap sensitiftas bakteri yaitu 85,7% (R=0,857) pada Escherichia coli, 91,1% (R=0,911) pada Salmonella typhi, dan 85,7% (R=0,857) pada Shigella sp.
Efektifitas Air Rebusan Daun Pandan (Pandanus Amaryllifolius) dalam Menghambat Pertumbuhan Bakteri Salmonella Typhi dengan Metode Dillution Test Edy Suwandi; Sugito Sugito
Jurnal Laboratorium Khatulistiwa Vol 1, No 1 (2017): November 2017
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v1i1.95

Abstract

Abstract: Pandanus leaf is one of the plants that contain active substances that can function as antibacterial. Active substances contained in pandan leaves can be used as traditional medicine in curing various diseases. This study was aimed to determine the effectiveness of boiled water of pandan leaves (Pandanus amaryllifolius) in inhibiting the growth of Salmonella typhi bacteria with the Dilution Test Method. The research design used was quasi-experiment with sampling technique used in this research was purposive sampling. Handling the sample by boiling then boiled water was diluted according to the desired concentration. The research was conducted at the Bacteriology Laboratory of Medical Laboratory of Health Ministry of Health Polytechnic Pontianak on February-April 2013. Then, data was processed by Friedman statistic test. Based on the research results of the effectiveness of boiled water of pandan leaves (Pandanus amaryllifolius) in inhibiting the growth of Salmonella typhi bacteria with Dilution Test Method can be concluded that the minimum inhibitory content of boiling water of pandan leaves effectively inhibits Salmonella typhi bacteria growth at concentration 60%. The statistical results show p-value of 0.000 (p = 0,000 <0,05), it can be concluded that pandan leaves boiling water is effective in inhibiting the growth of Salmonella typhi bacteria with dilution test method. Abstrak: Daun pandan merupakan salah satu tanaman yang mengandung zat-zat aktif yang dapat berfungsi sebagai antibakteri. Zat aktif yang terkandung pada daun pandan dapat digunakan sebagai obat tradisional dalam menyembuhkan berbagai macam penyakit. Penelitian ini bertujuan untuk mengetahui efektiftas air rebusan daun pandan (Pandanus amaryllifolius) dalam menghambat pertumbuhan bakteri Salmonella typhi dengan Metode Dillution Test. Desain penelitian yang digunakan adalah eksperimen semu dengan teknik pengambilan purposive sampling. Penanganan sampel dengan cara direbus kemudian air rebusan tersebut dilakukan pengenceran sesuai konsentrasi yang diinginkan. Penelitian dilakukan di Laboratorium Bakteriologi Jurusan Analis Kesehatan Poltekkes Kemenkes Pontianak pada bulan Februari - April 2013. Selanjutnya data diolah dengan Uji statistik Friedman. Berdasarkan hasil penelitian dapat disimpulkan bahwa kadar hambat minimum air rebusan daun pandan yang efektif menghambat pertumbuhan bakteri Salmonella typhi yaitu pada konsentrasi 60 %. Hasil statistik menunjukkan nilai p sebesar 0,000 (p = 0,000 < 0,05), maka dapat disimpulkan bahwa air rebusan daun pandan efektif dalam menghambat pertumbuhan bakteri Salmonella typhi dengan metode dillution test.
Perbedaan Hasil Pemeriksaan Mikro Hematokrit Menggunakan Makrosentrifus Dengan Mikrosentrifus Sri Tumpuk; Edy Suwandi
Jurnal Laboratorium Khatulistiwa Vol 1, No 2 (2018): Mei 2018
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v1i2.152

Abstract

Abstract: Hematocrit measurement is one of the most commonly special blood examination in laboratory to help in diagnosing various kind of diseases. Determination of hematocrit value can be done by macro and micro method. Macrocentrifus is multi function centrifuge with rotation speed reach to 3000 rpm for 30 minutes. Microcentrifus is a centrifuge for hematocrit only that used microcapiler tube with rotation speed of 1600 rpm for 5 minutes. This study aimed to identify the different hematocrit measurement micro method between macrocentrifus and microcentrifus. This study was type of analytical observation with research design was cross sectional approach. The study was done in June 2013. Sampling technique that has been applied was total population technique. Result in 48 samples accomplished the average of hematocrit measurement micro method in macrocentrifus was 43,33% meanwhile in microcentrifus was 42,85%. Data of study result analyzed by using t test in statistical program in order to determine whether there was a difference between those variables or not. Result analysis gained p value=0,000 (0,001) in 95% confidential interval due to p value 0,001< 0,05 consequently H0 refused and Ha accepted meaning that there was a significant difference between micro hematocrit result by using macrocentrifus and microcentrifus.Abstrak: Pemeriksaan hematokrit merupakan salah satu pemeriksaan darah khusus yang sering dikerjakan di laboratorium berguna untuk membantu diagnosa berbagai penyakit. Penetapan nilai hematokrit dapat dilakukan dengan cara makro dan mikro. Makrosentrifus adalah sentrifus multi fungsi dengan kecepatan pemusingan 3000 rpm selama 30 menit. Mikrosentrifus adalah sentrifus yang hanya digunakan untuk hematokrit yang menggunakan tabung mikrokapiler dengan kecepatan pemusingan 16000 rpm selama 5 menit. Penelitian ini bertujuan untuk mengetahui perbedaan hasil pemeriksaan hematokrit metode mikro menggunakan makrosentrifus dan mikrosentrifus. Jenis penelitian ini adalah observasi analitik dengan desain penelitian pendekatan cross sectional. Penelitian ini dilakukan bulan Juni 2013. Teknik pengambilan sampel yang digunakan adalah teknik total populasi. Hasil penelitian pada 48 sampel didapatkan rata-rata hasil pemeriksaan hematokrit metode mikro menggunakan makrosentrifus adalah 43,33% dan dengan mikrosentrifus adalah 42,85%. Data hasil penelitian dianalisa dengan uji t menggunakan program SPSS yang bertujuan untuk mengetahui adanya perbedaan pada kedua variabel tersebut. Hasil analisis didapatkan nilai  p = 0,000 (0,001) pada tingkat kepercayaan 95% Karena nilai p 0,001< 0,05 maka Ho ditolak dan Ha diterima   artinya ada perbedaan yang bermakna antara hasil pemeriksaan mikro hematokrit menggunakan makrosentrifus dengan mikrosentrifus.
Daya Hambat Air Perasan Rimpang Jeringau Merah Dan Rimpang Jeringau Putih Terhadap Pertumbuhan Bakteri Staphylococcus Aureus Metode Difusi Sari, Emilda; Triana, Linda; Suwandi, Edy
Jurnal Kesehatan Vol 13 No 2 (2022): Jurnal Kesehatan
Publisher : Poltekkes Kemenkes Tanjung Karang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26630/jk.v13i2.3081

Abstract

Jeringau merupakan tanaman tahunan yang mudah dibudidayakan, khususnya di daerah rawa atau di daerah dengan air. Jeringau merupakan tumbuhan yang banyak dimanfaatkan bagian rimpangnya dan secara empiris dipercaya dapat digunakan sebagai obat. Rimpang Jeringau memiliki aktivitas sebagai antibakteri, antijamur dan insektisida. Rimpang Jeringau menunjukkan adanya aktivitas penghambatan terhadap pertumbuhan bakteri Staphylococcus aureus. Rimpang Jeringau memiliki jenis yakni Jeringau Merah dan Jeringau Putih. Tujuan penelitian ini untuk menganalisis perbedaan daya hambat air perasan Rimpang Jeringau Merah dan Rimpang Jeringau Putih terhadap pertumbuhan bakteri Staphylococcus aureus metode difusi. Metode penelitian yang digunakan adalah eksperimen kuasi dengan teknik sampling purposive sampling. Populasi dalam penelitian ini adalah air perasan Rimpang Jeringau Merah dan Rimpang Jeringau Putih yang direplikasi masing-masing sebanyak 16 kali sehingga jumlah sampel dalam penelitian ini ada 32 sampel. Berdasarkan hasil penelitian didapatkan rerata zona hambat air perasan Rimpang Jeringau Merah 17,37 mm dengan kategori kuat dan rerata zona hambat air perasan Rimpang Jeringau Putih 10,19 mm dengan kategori lemah. Hasil analisis statistik Mann Whitney didapatkan nilai signifikansi p value 0,000 ≤ 0,05 sehingga dinyatakan bahwa hipotesis alternatif diterima yaitu terdapat perbedaan daya hambat air perasan jeringau merah dan jeringau putih terhadap pertumbuhan bakteri Staphylococcus aureus metode difusi.
Co-Authors Ari Nuswantoro Desi Wahyumarniasari Devi Nurfitri Bintang Dhantriviana, Indah Dinasti Aprillia Djohan, Herlinda Emilda Sari Emilda Sari Etiek Nurhayati Farchan Ananda Rizqial Febrianty, Resty Herlinda Djohan Hidayatussalihin Hidayatussalihin Ihsan, Bagus Muhammad Imma Fatayati Indah Dhantriviana Indrawati, Ratih Kamarudin Kamarudin Laila Kamilla Linda Triana Linda Triana Muarofah, Siti Najatun Muhammad Reza Setiawan Natasya Intan Ramadhani Nurhidayattulloh, Ariffialdi P Purwanto Putra, Rengga Khatulistiwa Ramadhani, Natasya Intan Resty Febrianty Reza Setiawan Rizqial, Farchan Ananda Saputra, Daniel Angkasa Sari, Emilda Siti Najatun Muarofah Slamet Slamet . Sri Tumpuk SUGITO Sugito - Sugito Sugito Sugito Sugito Sugito Sugito Sugito Sugito Sugito Sugito Sugito Sugito Supriyanto Supriyanto Supriyanto Supriyanto Supriyanto Supriyanto Supriyanto Supriyanto Sya’baniar, Luthfiranda Wahdaniah Wahdaniah Wahdaniah Wahyudi