The low level of Indonesian students’ science literacy as reflected in the PISA 2022 results highlights the need for innovative, contextualized learning media that can bridge scientific concepts with real-life and cultural experiences. This study aimed to develop and evaluate a Liveworksheet-integrated E-LKPD enriched with an ethno-vlog of Bukit Manengteung on ecology and ecosystem material to enhance students’ science literacy. Employing a research and development design with the 4D model (define, design, develop, and disseminate), the product was validated by experts, tested for readability, and implemented with seventh-grade students at SMP Negeri 2 Ciledug during the 2024/2025 academic year. Validation results indicated high feasibility with Aiken’s V values above 0.87, while student responses confirmed that the media was engaging, clear, and easy to use. Effectiveness tests showed a significant improvement in students’ science literacy, with pretest and posttest scores increasing from an average of 45.69 to 81.72 and an N-Gain of 0.68, categorized as medium to high. Improvements were observed across all science literacy dimensions, particularly in scientific attitudes, and the integration of ethno-vlog content enabled students to contextualize ecology concepts within their local cultural environment. These findings confirm that the developed media is valid, practical, and effective, with implications that integrating ethnoscience-based digital media into science instruction can enhance students’ scientific competencies while simultaneously preserving and strengthening cultural identity.
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