Jurnal Agrotek Tropika
Vol. 13 No. 3 (2025): JURNAL AGROTEK TROPIKA VOL 13, AGUSTUS 2025 (ON PROGRESS)

THE EFFECT OF TDZ (Thidiazuron) ON THE FORMATION OF DIRECT SOMATIC EMBRYOGENESIS IN Phalaenopsis sp. ORCHID

Adinda, Intan Dwi (Unknown)
Restanto, Didik Pudji (Unknown)
Dewanti, Parawita (Unknown)
Munandar, Denna Eriani (Unknown)
Hartatik, Sri (Unknown)
Prayoga, Mohammad Candra (Unknown)



Article Info

Publish Date
31 Aug 2025

Abstract

Orchids are ornamental plants that have a variety of beautiful flower colors. Optimization of clonal propagation through tissue culture techniques by adding appropriate growth regulators is important. This study aims to determine the optimal concentration of TDZ for the formation of somatic embryogenesis in Phalaenopsis sp. orchids which are studied through histological observationsand scanning electron microscopes. This study used a completely randomized design using the TDZ hormone with several concentrations (1 mg/L, 3 mg/L, 5 mg/L and 7 mg/L) which were added to MS media. The explants used were Phalaenopsis sp. leaves. Based on the results of the study, leaf explants successfully regenerated through direct somatic embryogenesis. The 5 mg/LTDZ treatment showed the best results in inducing somatic embryogenesis of Phalaenopsis sp. with the highest embryo percentage parameters of 32%, the fastest embryo maturity of 37 HST with dark green embryo characters and crumbly texture.

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Journal Info

Abbrev

JA

Publisher

Subject

Agriculture, Biological Sciences & Forestry

Description

Jurnal Agrotek Tropika (JAT) is a journal of science in the field of agrotechnology which covers several fields of science such as Agronomy, Horticulture, Soil Science, and Plant Pests and Diseases. Journal of Tropical Agrotek published since 2013 and published three times in one year ie in ...