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The Effect of Benzyl Amino Purin and NaphtalenaAcetic Acid Applications on Direct Shoot Organogenesis in Porang (Amorphophallus muelleri B) Restanto, Didik Pudji; Aji, Seto Purnomo; Handayani, Etty; Ratnasari, Tri; Jadmiko, Mochammad Wildan; Prayoga, Mohammad Candra; Khozin, Mohammad Nur; Kriswanto, Budi
PLANTA TROPIKA Vol 12, No 1 (2024)
Publisher : Department of Agrotechnology, Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.v12i1.18063

Porang (Amorphophallus muelleri B.) is a tuberous plant with the potential to be the main source of carbohydrates and is rich in benefits. Porang proliferation is limited by a 6-month dormancy period per year, and generative propagation is unlikely due to the seeds being apomictic triploid. The aimed of my research to analyze the application of BAP and NAA in culture media for direct propagation of porang shoots. The explant used in this research was young leaves. The research was arranged in a completely randomized design with a combination of BAP and NAA hormones added to the MS medium. There were three BAP treatments, namely 1.0 mg/L, 2.0 mg/L, and 3.0 mg/L, while NAA treatments consisted of 2 levels, namely 2.0 mg/L and 4.0 mg/L. The addition of 1.0 mg/L BAP combined with 4.0 mg/L NAA was the best treatment that produced seven shoots with an average shoot length of 2.14 cm and root length of 3.6 cm, with the earliest bud emergence (9.7 weeks after planting).

Multiplikasi Tunas pada Eksplan Rimpang Kunyit Hitam (Kaempferia parviflora) dengan Penambahan Hormon BAP Restanto, Didik Pudji; Hanifah, Fairuz Luthfi; Prayoga, Mohammad Candra; Zahro, Fauziatuz; Khozin, Mohammad Nur
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 3 (2024): October 2024
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v9i3.8534

Kunyit Hitam (Kaempferia parviflora) adalah tanaman rimpang berwarna ungu kehitaman yang banyak manfaat sebagai bahan obat tradisional. Perbanyakan bibit kunyit hitam secara konvensional tidak memungkinkan untuk budidaya skala besar, dikarenakan membutuhkan waktu yang cukup lama dan tidak efisien. Rendahnya pasokan bahan tanaman berkualitas dan masalah perkecambahan yang rendah menjadi dasar dalam upaya pengembangan kunyit hitam secara in vitro. Perbanyakan kunyit hitam melalui multiplikasi tunas dapat mempercepat produksi bibit yang efisien. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian berbagai konsentrasi hormon BAP terhadap multiplikasi kunyit hitam. Rancangan penelitian menggunakan RAL dengan faktor konsentrasi hormon BAP yang terdiri dari 5 taraf konsentrasi antara lain BAP 0 mg/L (P0), BAP 2 mg/L (P1), BAP 4 mg/L (P2), BAP 6 mg/L (P3), dan BAP 8 mg/L (P4). Hasil penelitian menunjukkan bahwa, perlakuan BAP 2 mg/L memperlihatkan hasil terbaik dengan kedinian tunas tercepat yaitu 8,3 hari setelah tanam, jumlah tunas terbanyak yaitu 5,8 tunas, rata-rata tunas tertinggi mencapai 4,8 cm, dan jumlah daun terbanyak yaitu 8,3 helai. Perlakuan BAP 2 mg/L menghasilkan persentase tunas dan akar 100%.

Optimasi Regenerasi Kalus Embriogenik Porang (Amorphophallus muelleri Blume) Melalui Kultur Suspensi Sel Menggunakan BAP (Benzyl Amino Purin) Restanto, Didik Pudji; Prayoga, Mohammad Candra; Arsyika, Izna; Farlisa, Veronenci Yuliarbi; Hardjo, Popy Hartatie
Vegetalika Vol 13, No 4 (2024)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/veg.100708

Porang Amorphophallus muelleri Blume merupakan tanaman umbi-umbian yang banyak dimanfaatkan sebagai bahan kosmetik dan diekspor untuk bahan baku industri. Perbanyakan porang secara konvensional mengalami masa dormansi. Perbanyakan porang melalui kultur jaringan dapat menghasilkan bibit yang cepat dan dalam jumlah yang banyak. Kultur jaringan porang dapat melalui somatik embriogenesis melalui pembentukan kalus dan poliferasi kalus dengan kultur suspensi sel. Optimasi regenerasi kalus hasil poliferasi kultur suspensi sel sangat penting untuk menunjang keberhasilan perbanyakan porang secara masal. Penelitian ini bertujuan untuk mengetahui pengaruh konsentrasi BAP terhadap regenerasi kalus embriogenik porang Amorphophallus muelleri B. hasil kultur suspensi. Penelitian menggunakan rancangan acak lengkap dengan faktor konsentrasi BAP yang terdiri dari 4 taraf perlakuan antara lain 1 mg.L-1, 2 mg.L-1, 3 mg.L-1, dan 4 mg.L-1. Setiap perlakuan diulang sebanyak 5 ulangan. Analisis data penelitian menggunakan ANOVA dan uji lanjut dengan DMRT pada taraf kesalahan 5%. Analisis data menggunakan aplikasi SPSS statistics versi 26. Berdasarkan hasil penelitian, perlakuan BAP 1 mg.L-1menunjukkan hasil terbaik dengan parameter kedinian munculnya tunas tercepat 45 hst, tunas terpanjang 7.6 cm, jumlah tunas terbanyak 7,33 tunas, dan persentase regenerasi 93,33%. Berdasarkan pengamatan histologi, kalus embriogenik berkembang menghasilkan mature embrio yang lebih banyak sehingga dapat menginduksi tunas yang banyak.

The Effect of Benzyl Amino Purine (BAP) and Coconut Water on the Growth of Vanilla (Vanilla planifolia Andrews.) In Vitro Kusbianto, Dwi Erwin; Haliza, Nurhayadatul; Restanto, Didik Pudji; Wulanjari, Distiana; Avivi, Sholeh; Prayoga, Mohammad Candra
Jurnal Natur Indonesia Vol. 22 No. 2 (2024): October
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jnat.22.2.76-83

Vanilla (Vanilla planifolia Andrews.) is one of the plantation crops that has a high selling price, namely wet vanilla IDR 200,000-300,000/kg, regular quality dry vanilla around 1-3 million/kg, and export quality reaching 5-7 million/kg. The opportunity for farmers to develop vanilla commodities in Indonesia is very large, but limited planting materials still hamper it. In-vitro propagation is one technique that can be used to overcome the problem of vanilla propagation in Indonesia. This research aimed to determine the interaction of BAP and coconut water on the growth of vanilla nodal explants in vitro. The method used in this research was a factorial Completely Randomized Design (CRD) with a combination of Benzyl Amino Purine (BAP) and coconut water. The BAP concentration consists of 0 mg/L, 1 mg/L, and 2 mg/L. Coconut water has 0%, 15%, and 30% levels. Data were analyzed using ANOVA and further tested with the DMRT test at a confidence level of 95%. The results showed an interaction between BAP and coconut water on the growth of vanilla explants. The combination treatment of 1 mg/L BAP + 15% coconut water showed the best results with the fastest response time of 8.76+1.53 DAP, average shoot length 2.84±0.70 cm/explant and average root length 0.75±0.07 cm/explant.

Shoots induction of axillary buds in blueberries (Vaccinium corymbosum L.) in vitro with the addition of BAP and IBA Restanto, Didik Pudji; Budha, Al Sura Tri; Prayoga, Mohammad Candra; Slameto, Slameto; Khozin, Mohammad Nur
Biogenesis: Jurnal Ilmiah Biologi Vol 12 No 2 (2024)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v12i2.46312

Blueberry Vaccinium corymbosum L. is a fruit plant that has high economic value and many benefits. Blueberries in Indonesia are not yet in great demand so domestic needs are still met by imports. Conventional blueberry propagation is done using stem cuttings. The disadvantages of conventional propagation are that seedling growth is slow, requires a large source of planting material, and is ineffective. The aim of the research was to determine the effect of the combination of BAP and IBA hormones on the induction of axillary shoots in effective in vitro propagation of blueberries. The explants used were axillary buds of blueberry, sterilized using fungicide, bactericide, bayclin, sodium hypochlorite and alcohol. The research design used a completely randomized design (CRD) with a combination of BAP and IBA hormones. The BAP hormone consists of three concentrations, namely 0.5, 1.0, and 1.5 mg/L. The IBA hormone consists of three concentrations, namely 0, 0.1, and 0.2 mg/L. Based on research results, the combination treatment of BAP and IBA succeeded in inducing shoots, but was not able to induce roots. The BAP 0.5 mg/L treatment showed the best results with a shoot emergence rate of 4.33 weeks, shoot height of 1.68 cm, number of shoots 1.67, and number of leaves 12.67. The findings of this study highlight the potential of BAP and IBA hormone combinations in accelerating blueberry shoot induction, offering an alternative propagation method that could support domestic production and reduce dependence on imports.

The Effect of Media Composition on the Growth of Fig (Ficus carica L.) Grafts Restanto, Didik Pudji; Antono, Moh Nuri; Setiyono, Setiyono; Muhlison, Wildan; Prayoga, Mohammad Candra; Zahro, Fauziatuz
Journal of Applied Agricultural Science and Technology Vol. 9 No. 3 (2025): Journal of Applied Agricultural Science and Technology
Publisher : Green Engineering Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55043/jaast.v9i3.413

The fig plant is cultivated for its fruit due to its high economic value. Vegetative propagation can serve as an alternative for mass propagation of fig plants, such as grafting. Vegetative propagation of figs is conducted in several ways, including grafting and cuttings. The problems encountered in grafting are mainly caused by unsuitable media conditions. This research aims to determine the best composition of grafting media for fig plants. The study was conducted in Dwiwangsa Tin Garden Park, Sidomulyo Village, Krian District, Sidoarjo Regency, East Java. Rainfall at the research site was classified as dry season, with 0 mm in October 2023, 7 mm in November 2023, 150 mm in December 2023, 211 mm in January 2024, and 485 mm in February 2024. The research employed a randomized block design (RBD) consisting of nine media combination treatments. The treatments included soil medium, husk medium, husk charcoal medium, cocopeat medium, sawdust sewing powder medium, a combination of soil and husk medium (1:1), a combination of soil and charcoal husk medium (1:1), a combination of soil and cocopeat medium (1:1), and a combination of soil and sawdust medium (1:1). Each grafting medium showed a different growth response in terms of root number. Based on the results, the husk charcoal medium produced the highest values, with 89.8 roots, root length of 7.24 cm, root diameter of 1.38 cm, root fresh weight of 5.22 g, root volume of 7.7 ml, and root dry weight of 0.37 g.

THE EFFECT OF TDZ (Thidiazuron) ON THE FORMATION OF DIRECT SOMATIC EMBRYOGENESIS IN Phalaenopsis sp. ORCHID Adinda, Intan Dwi; Restanto, Didik Pudji; Dewanti, Parawita; Munandar, Denna Eriani; Hartatik, Sri; Prayoga, Mohammad Candra; Solikhah, Ummi
Jurnal Agrotek Tropika Vol. 13 No. 3 (2025): JURNAL AGROTEK TROPIKA VOL 13, AGUSTUS 2025
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jat.v13i3.9795

Orchids (Phalaenopsis sp.) are among the most valuable ornamental plants due to their wide range of flower colors and high commercial demand. However, conventional propagation methods are often inefficient for large-scale production, necessitating the optimization of tissue culture techniques for rapid and uniform clonal propagation. The use of appropriate plant growth regulators, particularly thidiazuron (TDZ), is crucial for enhancing somatic embryogenesis—a key pathway for orchid micropropagation. This study aimed to determine the optimal concentration of TDZ for inducing somatic embryogenesis in Phalaenopsis sp. through histological analysis and scanning electron microscopy (SEM) observations. A completely randomized design was employed using Murashige and Skoog (MS) medium supplemented with four TDZ concentrations (1, 3, 5, and 7 mg/L). Leaf explants of Phalaenopsis sp. served as the explant source. The results demonstrated that direct somatic embryogenesis successfully occurred from leaf explants across treatments. Among the tested concentrations, 5 mg/L TDZ produced the most effective response, resulting in the highest somatic embryo formation rate (32%) and the shortest time to embryo maturation (37 days after culture initiation). The embryos exhibited characteristic dark green coloration and a crumbly texture. These findings highlight the pivotal role of TDZ in promoting somatic embryogenesis in Phalaenopsis sp., providing a reliable protocol for efficient orchid propagation. The study contributes to the advancement of orchid biotechnology by offering histological and ultrastructural evidence that supports the optimization of clonal propagation systems for commercial and conservation purposes.

Direct Organogenesis of Different Explants of Aceh Patchouli (Pogostemon cablin Benth.) with Several BAP Concentrations Prayoga, Mohammad Candra; Soeparjono, Sigit; Dewanti, Parawita; Handoyo, Tri; Hardjo, Popy Hartatie; Restanto, Didik Pudji
Biosaintifika: Journal of Biology & Biology Education Vol. 16 No. 3 (2024): December 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v16i3.12839

The patchouli plant (Pogostemon cablin Benth.) is a tropical herbaceous plant that produces essential oil. One of the problems is that the production is not yet optimal. Fulfillment of superior seedlings can help increase patchouli productivity. Conventional patchouli propagation through stem cuttings is ineffective and takes longer. Patchouli propagation can be done using a tissue culture approach via direct organogenesis to produce seedlings quickly and efficiently. Effective patchouli propagation methods and successful acclimatization are very important to research to support the propagation and breeding of patchouli plants. The aim of this research was to determine the best of BAP concentration in direct organogenesis of leaf and stem explants. The research design used a completely randomized series of hormone BAP, it has 5 levels, namely 0 mg/L (as control), 0.25 mg/L, 0.50 mg/L, 0.75 mg/L, and 1.0 mg/L. The explants used were the leaves and stems of Aceh patchouli. Plantlets are acclimatized in compost media and covering treatment. Based on the results of observations, the best BAP concentration is 0.25 mg/L with the initial observation parameters of the early emergence of shoots, number of shoots, and length of shoots on leaf explants were 10 daps, 35.33 shoots, and 2.83 cm respectively. The use of leaf explants showed a better response compared to stem explants. Patchouli plantlets were successfully acclimatized and can adapt to the ex vitro environment using the covering method. Successful patchouli propagation and high acclimatization can help produce effective patchouli seeds.

Multiplikasi Tunas pada Eksplan Rimpang Kunyit Hitam (Kaempferia parviflora) dengan Penambahan Hormon BAP Restanto, Didik Pudji; Hanifah, Fairuz Luthfi; Prayoga, Mohammad Candra; Zahro, Fauziatuz; Khozin, Mohammad Nur
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 3 (2024): October 2024
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v9i3.8534

Kunyit Hitam (Kaempferia parviflora) adalah tanaman rimpang berwarna ungu kehitaman yang banyak manfaat sebagai bahan obat tradisional. Perbanyakan bibit kunyit hitam secara konvensional tidak memungkinkan untuk budidaya skala besar, dikarenakan membutuhkan waktu yang cukup lama dan tidak efisien. Rendahnya pasokan bahan tanaman berkualitas dan masalah perkecambahan yang rendah menjadi dasar dalam upaya pengembangan kunyit hitam secara in vitro. Perbanyakan kunyit hitam melalui multiplikasi tunas dapat mempercepat produksi bibit yang efisien. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian berbagai konsentrasi hormon BAP terhadap multiplikasi kunyit hitam. Rancangan penelitian menggunakan RAL dengan faktor konsentrasi hormon BAP yang terdiri dari 5 taraf konsentrasi antara lain BAP 0 mg/L (P0), BAP 2 mg/L (P1), BAP 4 mg/L (P2), BAP 6 mg/L (P3), dan BAP 8 mg/L (P4). Hasil penelitian menunjukkan bahwa, perlakuan BAP 2 mg/L memperlihatkan hasil terbaik dengan kedinian tunas tercepat yaitu 8,3 hari setelah tanam, jumlah tunas terbanyak yaitu 5,8 tunas, rata-rata tunas tertinggi mencapai 4,8 cm, dan jumlah daun terbanyak yaitu 8,3 helai. Perlakuan BAP 2 mg/L menghasilkan persentase tunas dan akar 100%.

Pengaruh 2,4-D dan BAP terhadap Multiplikasi Tanaman Vanili (Vanilla planifolia Andrews): Pengaruh 2,4-D dan BAP terhadap Multiplikasi Tanaman Vanili (Vanilla planifolia Andrews) Restanto, Didik Pudji; Nadiya, Nisma Riyadh; Dewanti, Parawita; Khozin, Mohammad Nur; Prayoga, Mohammad Candra
AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Vol 9 No 2 (2025): AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian
Publisher : Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/agrosainstek.v9i2.912

Vanilla is a plant that has many benefits, high selling value and potential to be developed. Conventional propagation of vanilla such as through cuttings has not been effective, so a more effective method of propagation is needed, one of which is propagation through tissue culture. In-vitro propagation of vanilla can be through shoot multiplication to produce fast plantlets. One of the successes of shoot multiplication is influenced by the hormone given. This study aims to determine the effect of giving a combination of 2,4-D and BAP on the multiplication of vanilla shoots. The study design used CRD with a combination factor of 2,4-D (concentration 0.5 mg/L, 1.0 mg/L, and 1.5 mg/L) and BAP (concentration 0.25 mg/L, 0.5 mg/L, and 0.75 mg/L). The explants used were vanilla stem segments. Based on the research results, the combined treatment with concentrations of 2,4-D 1 mg/L and BAP 0.5 mg/L had a very significant effect on the multiplication of vanilla shoots with the fastest emergence of shoots with an average of 6.6 wap, the highest number of shoots was 7 shoots, and the survival percentage was 100%.

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