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All Journal Vegetalika Jurnal Ilmu Pertanian Agrikultura Jurnal Ilmu Dasar Jurnal Natur Indonesia AL KAUNIYAH Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Agrovigor: Jurnal Agroekoteknologi Biogenesis: Jurnal Ilmiah Biologi CEMARA Journal of Degraded and Mining Lands Management AGRIVITA, Journal of Agricultural Science Planta Tropika UNEJ e-Proceeding Jurnal Penelitian Pertanian Terapan Agriprima : Journal of Applied Agricultural Sciences Jurnal Penelitian Pendidikan IPA (JPPIPA) Agrin : Jurnal Penelitian Pertanian SELAPARANG: Jurnal Pengabdian Masyarakat Berkemajuan Jurnal Agrotek Tropika Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science) Jurnal Pengabdian Magister Pendidikan IPA Jurnal Kreativitas PKM jurnal Ilmu Pertanian (Agricultural Science) J-Dinamika: Jurnal Pengabdian Kepada Masyarakat Jurnal Agrotek Indonesia Agroteknika Agro Bali: Agricultural Journal Jurnal Pengabdian Masyarakat IPTEKS Jurnal Bioindustri Jurnal Pengabdian Masyarakat Sasambo BIOVALENTIA: Biological Research Journal Journal of Applied Agricultural Science and Technology Prosiding Seminar Nasional Pengabdian Kepada Masyarakat PAPUMA: Journal of Comunnity Services Journal of Bioindustry Jurnal Natur Indonesia
Restanto, Didik Pudji
Prodi Agronomi, Fakultas Pertanian, Universitas Jember
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Cloning coat protein gene of CBSD (cassava brown streak disease) at cassava (Manihotesculentum) Restanto, Didik Pudji; ., Slameto; Kriswanto, Budi; Addy, Hardian Susilo; Handoyo, Tri
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

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Abstract

Cassava Brown Streak Disease (CBSD) is a major disease in cassava plants which have the serious problems in cassava plantations in the world, especially in Africa, Tanzania and India (Wassawaet., al, 2010). In Indonesia, the virus is still not optimal yet in the handling. The disease is present in plants that can destructive cassava leaves, stems and tubers.  It was greatly reduces the quality and production in the world such as India.  The decrease of cassava yield can reach 100% due to disease of CBSD (Lopez, 2003). The primer was designed from the coat protein gene of CBSD with a distance of 380 bp (Abarshiet.,al, 2012). The primers designed the forward primer (GGARCCRATGTAYAAATTTGC) and Reverse (GCWGCTTTTA  TYACAAAMGC). The RNA isolation have been used Plant Virus RNA Kit (Geneaid).  The CBSD RNA concentration around 55,2ng/ul.  The RT PCR program were one cycle of RT PCR reaction (45oC for 30 min), denaturation (45oC for 5 min) and 30 cycles for denaturation (94oC for 1 min), annealing (52oC for 30 sec), extention  (72oC for 1 min).  The results showed a single band of about 380 bp which is the  distance between the two primers were tested.  The multiplication shoot around 5 shoots per meristem explants with a combination of 0.5 ppm and 0.1 ppm BAP GA3 Keyword : Cassava Brown Streak Disease (CBSD), CASSAVA (Manihotesculentum), RT PCR
KAJIAN THIDIAZURON (TDZ) DALAM INDUKSI PLB ANGGREK Phalaenopsis sp SECARA IN VITRO Restanto, Didik Pudji; Kriswanto, Budi; Khozim, Mohammad Nur; Soeparjono, Sigit
AGRITROP Vol 16, No 1 (2018): Agritrop : Jurnal Ilmu-Ilmu Pertanian
Publisher : Universitas Muhammadiyah Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (763.329 KB) | DOI: 10.32528/agr.v16i1.1561

Abstract

Anggrek Phalaenopsis sp merupakan jenis anggrek endemik di hutan tropis Kalimantan yang mempunyai nilai eksotik yang tinggi. Indonesia adalah negara tropis yang mempunyai plasma nutfah anggrek sekitar 6000.  Pengembangan anggrek ini di Indonesia masih secara tradisional sehingga belum mampu bersaing dengan negara lain Taiwan, Malaysia, Singgapur dan china.  Perkembangan anggrek akan lebih pesat dengan kemajuan teknologi yaitu sistem bioreaktor sehingga mampu menghasilkan dalam sekala industri.  Tujuan dari pada penelitan ini adalah ingin mengetahui peran TDZ dalam perkembangan induksi PLB. Pemberian TDZ 1 ppm meningkatkan berat basah PLB dan jumlah PLB sebesar masing-masing 34 g dan 40.  Pada perlakuan kontrol (tanpa TDZ) akan membentuk sistem perakaran yaitu jumlah akar (5,5) dan panjang akar ( 16 cm).Sebaliknya, dengan pemberian TDZ justru tidak memunculkan system perakaran melainkan terjadi perkembangan PLB yang terjadi.
Pengaruh Ukuran Embriozigot terhadap Regenerasi Beberapa Klon Kakao Avivi, Sholeh; Restanto, Didik Pudji; Widyastuti, Tri
Jurnal Natur Indonesia Vol 13, No 3 (2011)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (181.245 KB) | DOI: 10.31258/jnat.13.3.237-243

Abstract

This sesearch identified the responses of size of zygotic embryo of cocoa and to identify the responses of cocoaclone types toward ability to produce somatic embryo. The research was designed by Completely RandomizedFactorial Design within five replications. The first factors were the sizes of used explants, namely small (2-5 mm),average (6-9 mm) and big (10-15 mm). The second factors were cocoa clones, namely RCC 72, Sca 6, KW 162, KW163 and KW 165. The result showed that the best explant was the small size (2-5 mm) of cocoa zygotic embryo. Ithad significantly on number of rooting embryos and number of embryos with buds on rooting media. Clone RCC 72was the most responsive clone and could generate to be plantlet. Clone KW 165 performed the lowest response.
Morphological Diversity and Molecular RAPD Markers of Sugarcane Mutane (Saccharum officinarum L.) in Inundation Tolerance Avivi, Sholeh; Suliswanto, Eko Nur; Restanto, Didik Pudji; Miswar, Miswar; Syamsunihar, Anang; Soeparjono, Sigit; Hartatik, Sri
AGRIVITA, Journal of Agricultural Science Vol 41, No 2 (2019)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v41i2.1304

Abstract

The study aimed to identify variations in morphological and molecular character of sugarcane mutants from the mutation of Ethyl Methane Sulphonate (EMS). It used 21 sugarcane mutants and two non-mutant PS865 plants as controls. The treatments with inundation were carried out on 1-17 mutants and non-mutants, while the treatments without inundation were carried out on 18-21 mutant plants and non-mutants. The tolerance characteristics base on the agronomic characters. The RAPD molecular character was observed to detect changes in genotypes and kinship relationships of the plants tested. The results showed that the characteristics of tolerance to the best inundation were found in mutants 1, mutants 3 and mutants 6 which were characterized by the root volume (cm3), fresh root weight (g), sucrose content and brix value (%). Mutant plants treated with inundation showed higher levels of sucrose and brix values. Whereas the control plants in fats, showed lower levels of sucrose and brix. Changes in sugarcane mutant genotypes from non-mutants based on RAPD markers ranged from 14.7 - 56.7 % which resulted in an average polymorphic band of 35.1 % from 37 DNA bands and produced four main groups based on dendrogram analysis.
MORPHOLOGICAL CHARACTERISTICS AND POLLINIA OBSERVATION OF 10 INDONESIA NATIVE DENDROBIUM ORCHIDS Indraloka, Aldy Bahaduri; Dewanti, Parawita; Restanto, Didik Pudji
BIOVALENTIA: Biological Research Journal Vol. 5 No. 2 (2019)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1067.872 KB) | DOI: 10.24233/BIOV.5.2.2019.140

Abstract

Characterization of native orchid (species) in Indonesia is important for preservation of germplasm and selection process to obtain dominant character of orchids as potential parental. Morphological characterization is a method to obtain scientific information in phenotypic manner. Characterization of plants was carried out on 10 native orchids of Dendrobium genus to obtain specific character. Characterization process based on UPOV guidebook and morphological observation of pollinia was also carried out on 10 native orchids to obtain information about the structure and physiology of pollinia. The results of morphological characterization on 10 native orchid showed several qualitative characters such as flowering position, cross section of leaf, pseudobulb firmness, and leaf arrangement in D. Canaliculatum was different from 9 other native orchid. Morphological pollen of 10 native orchid shows the diversity structure of pollinia and the physiological conditions of mature pollen which are ready to pollinate are pollinia with a golden yellow colored. Knowledge and information about parental relationships is the main point for developing ornamental plant breeding programs. Selection of native orchids as potential elder is important where selected elders will be used as a source of genetic diversity. The results of this study are expected to provide scientific information and phenotypic selection for the development of hybrid orchids.
PRIMACY OF LIQUID MEDIUM TECHNIQUE ON PROTOCORM LIKE BODIES PROPAGATION OF Phalaenopsis sp ORCHIDS IN TISSUE CULTURE Kriswanto, Budi; Soeparjono, Sigit; Restanto, Didik Pudji
BIOVALENTIA: Biological Research Journal Vol. 6 No. 1 (2020)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1283.959 KB) | DOI: 10.24233/BIOV.6.1.2020.169

Abstract

Tissue culture have been used for plant propagation generally, and the medium has been important role in its growth. Vegetative propagation on Phalaenopsis sp orchids can be through the protocorm like bodies (PLB). Medium of affect on propagation of PLB was carried out on medium type, kind of basal medium and concentrations ratio of naphtaleneacetic acid (NAA) and benzylamino purine (BAP). The experiment used Completely Randomized Factorial Design with 3 replications and continued with the Duncan Multiple Range Test (DMRT) if there were significant differences. The results showed that the best callus formed in a combination of solid medium type and Murashige & Skoog (MS) basal medium was 100%. The most number of PLB produced from a combination of liquid medium types and a concentration ratio of NAA 1 mgL-1 and BAP 5 mgL-1, the most number of plantlet produced from a combination of MS basal medium and the concentrations ratio of NAA 0.1 mgL-1 and BAP 0.1 mgL-1, the number of PLB germination and PLB with leaves were influenced by each single factor.
Pengaruh Naphthalene Acetic Acid (NAA) dan Kinetin terhadap Perkembangan Protocorm-Like Body (PLB) dan Regenerasi Anggrek Phalaenopsis sp. Hybrid Didik Pudji Restanto; Budi Kriswanto; Nafisah Iqmatullah; Parawita Dewanti
Agrikultura Vol 32, No 2 (2021): Agustus, 2021
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/agrikultura.v32i2.32095

Abstract

Phalaenopsis merupakan anggrek yang mempunyai nilai ekonomis tinggi karena warna yang menarik. Permintaan selalu meningkat sehingga  tehnologi kultur jaringan sangat dibutuhkan dalam perbanyakan anggrek ini. Perbanyakan tanaman anggrek menggunakan sumber eksplan protocorm-like body (PLB) merupakan salah satu cara yang tepat untuk menghasilkan anggrek dalam jumlah banyak. Penelitian ini bertujuan untuk mendapatkan kombinasi NAA dan Kinetin terbaik pada perkembangan PLB di kultur cair serta PLB yang disubkultur di media regenerasi. Bahan tanam menggunakan PLB umur 2 bulan yang ditanam pada media Vacin & Went (VW) cair dengan perlakuan kombinasi NAA dan Kinetin masing-masing pada konsentrasi 2,5 mg/L, 5 mg/L dan 7,5 mg/L. Media regenerasi menggunakan VW padat dengan suplemen 15% air kelapa, 5% pisang, 5% kentang dan 0,2% arang aktif. Percobaan menggunakan Rancangan Acak Lengkap dengan tiga kali ulangan. Hasil penelitian menunjukkan bahwa kombinasi NAA dan kinetin tidak berpengaruh terhadap jumlah dan berat PLB. Sementara itu, warna PLB hampir semua menunjukkan warna yang sama yaitu hijau kekuningan (5GY 7/6) hanya pada kombinasi perlakuan NAA 5 mg/L + Kinetin 7,5 mg/L terjadi klorosis berwarna kuning pucat (5Y8/4).  Perubahan warna planlet terjadi dari hijau kekuningan terang (7.5GY8/8) menjadi hijau kekuningan (7.5GY7/6) dan hijau kekuningan tua (7.5GY6/10) masing-masing pada awal aklimatisasi, serta pada dua dan empat minggu. Bibit anggrek tumbuh dengan baik dan menjadi tanaman dewasa setelah dipindahkan pada media moss hitam.
Pengaruh cekaman genangan terhadap pertumbuhan dan hasil beberapa varietas tomat (Lycopersicum esculentum Mill.) Sholeh Avivi; Aprila Iga Mufidah; Tri Agus Siswoyo; Didik Pudji Restanto
Agrovigor Vol 15, No 1 (2022): Maret
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agrovigor.v15i1.8866

Abstract

Usaha untuk meningkatkan produksi tomat pada lahan tercekam genangan dapat dilakukan dengan menguji varietas-varietas tomat yang sudah beredar terhadap cekaman genangan. Uji dilakukan dengan mengamati parameter pertumbuhan dan hasil beberapa varietas tomat. Penelitian ini bertujuan untuk mengetahui toleransi beberapa varietas tomat terhadap cekaman genangan. Metode yang digunakan pada penelitian ini adalah Rancangan Acak Kelompok (RAK) faktorial dengan 3 kali ulangan. Varietas tanaman tomat merupakan faktor pertama dengan 5 taraf yaitu a) V1 Sakina F1, b) V2 Tora, c) V3 Rewako F1, dan d) V4 Mawar. Sedangkan faktor yang kedua adalah cekaman genangan dengan 3 taraf, yaitu a) T1, perlakuan kondisi cekaman genangan 5 cm, b) T2, perlakuan kondisi cekaman genangan 10 cm, c) T3, perlakuan kondisi cekaman genangan 15 cm. Hasil penelitian menunjukkan bahwa varietas Rewako mempunyai karakter lebih toleran terhadap genangan yang ditunjukkan pada parameter rerata tinggi tanaman, ukuran diameter buah, berat buah dan berat tajuk atas tanaman. 
MORPHOLOGICAL AND PHYSIOLOGICAL CHARACTERS OF CASSAVA (MANIHOT ESCULENTA CRANTZ) WHICH WET TOLERANT Rahmawati Rahmawati; Tri Agus Siswoyo; Didik Puji Restanto; Sri Hartatik; Sigit Soeparjono; Sholeh Avivi
UNEJ e-Proceeding 2016: Proceeding The 1st International Basic Science Conference
Publisher : UPT Penerbitan Universitas Jember

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Abstract

Weather conditions often unstable changed and increase the floods of agricultural land. Land which was originally drought will experience wetness and need variety of a plant that wetness tolerant. This study were aimed to get cassava that wet tolerant and to study the morphological, physiological characters of cassava grown on wetness land. The experiment was conducted based on factorial random plot design that consisting two factors with five replications. The first factor were four varieties namely: V1 = Daun Ganja; V2 = Sawi Ketan; V3 = Kasesat Beracun; V4 = Gajah. The second factor was wet treatment consisting of 2 the condition that were K0 = as control, media with 100% field capacity; K1 = as wet treatment by watering of media maintain on -10 cm from media surface. The difference between treatments tested with Duncan Multiple Range Test (DMRT) with the probability 5%. Wet treatment started when plant aged was 2 months after planting, during eight weeks. The observations on variables based on morphological and physiological characters. Result showed that every varieties give the different response on wet treatment. Best response on wet tolerant variety shown by variety code of V2 (Sawi Ketan) were indicated by plant height, total leaf number produced, stem diameter, fresh weight of root, dry weight of root, fresh weight of shoot, dry weight of shoot, photosynthesis index, stomata conductivity and root volume.
Cloning coat protein gene of CBSD (cassava brown streak disease) at cassava (Manihotesculentum) Didik Pudji Restanto; Slameto .; Budi Kriswanto; Hardian Susilo Addy; Tri Handoyo
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UPT Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Cassava Brown Streak Disease (CBSD) is a major disease in cassava plants which have the serious problems in cassava plantations in the world, especially in Africa, Tanzania and India (Wassawaet., al, 2010). In Indonesia, the virus is still not optimal yet in the handling. The disease is present in plants that can destructive cassava leaves, stems and tubers.  It was greatly reduces the quality and production in the world such as India.  The decrease of cassava yield can reach 100% due to disease of CBSD (Lopez, 2003). The primer was designed from the coat protein gene of CBSD with a distance of 380 bp (Abarshiet.,al, 2012). The primers designed the forward primer (GGARCCRATGTAYAAATTTGC) and Reverse (GCWGCTTTTA  TYACAAAMGC). The RNA isolation have been used Plant Virus RNA Kit (Geneaid).  The CBSD RNA concentration around 55,2ng/ul.  The RT PCR program were one cycle of RT PCR reaction (45oC for 30 min), denaturation (45oC for 5 min) and 30 cycles for denaturation (94oC for 1 min), annealing (52oC for 30 sec), extention  (72oC for 1 min).  The results showed a single band of about 380 bp which is the  distance between the two primers were tested.  The multiplication shoot around 5 shoots per meristem explants with a combination of 0.5 ppm and 0.1 ppm BAP GA3 Keyword : Cassava Brown Streak Disease (CBSD), CASSAVA (Manihotesculentum), RT PCR
Co-Authors . Usmadi Adinda, Intan Dwi Agnes Natalia Wijaya Aji, Seto Purnomo Alfarisy, Fariz Kustiawan Anang Syamsunihar Antono, Moh Nuri Aprila Iga Mufidah Arsyika, Izna Arya Wiranegara Azizah, Kunti Anis Bambang Kuswandi Bambang Sugiharto Bintoro, Jatmiko Hardi Budha, Al Sura Tri Budi Kristanto Budi Kriswanto Budi Kriswanto Budi Kriswanto Budi Kriswanto Budi Kriswanto, Budi Calista, Zalza Candra Prayoga, Mohammad Darah Pertami, Rindha Rentina Dinata, Gallyndra Fatkhu Dodi Wijaya Dwi Erwin Kusbianto Dwika Nano Hariyanto Endah Cahyani Simamora Farlisa, Veronenci Yuliarbi Fenny Irawati Fifteen Aprila Fajrin Firdauzi, Sandy Al Firdha Narulita Alfian Hadi Paramu Haliza, Nurhayadatul Handayani, Etty Hanifah, Fairuz Luthfi HARDIAN SUSILO ADDY Harsanti, Restiani Sih Indraloka, Aldy Bahaduri Kacung Hariyono Ketut Anom Wijaya Khozim, Mohammad Nur Khozin, Mohammad Nur Kriswanto, Budi Kushariyadi Kushariyadi Laily Ilman Widuri, Laily Ilman Laksono Trisnantoro Luluk Noviana Lutfi Pramukyana Mochammad Wildan Jadmiko Mohammad Nur Khozim Mohammad Nur Khozin Mohammad Nur Khozin Mona, Muhammad Dima Say Muhammad Dima Say Mona Muhammad Nur, Kholid Rosyidi Munandar, Denna Eriani Murtaqib Murtaqib Nafisah Iqmatullah Natasha Florenika Nur Lailin Nafiah Nurfika Asmaningrum Nurhasna, Rifqi Pamungkas, Wahyu Eka Parawita Dewanti Parawita Dewanti Parawita Dewanti Poppy Hartatie Hardjo Popy Hartatie Hardjo Prayoga, Mohammad Candra Putri, Widya Kristyanti Rahmawati Rahmawati Raisah Bani Ratnasari, Tri Refa Firgiyanto Refa Firgiyanto Resti, Ricke Windi Rizqi Maulana Rusdiana, Riza Yuli Saputro Gumelar, Bachtiar Setiyono Setiyono Sholeh Avivi Sigit Soepardjono Sigit Soeparjono Slameto . Slameto Slameto Slameto Slameto Slameto Slameto Sri Hartatik Suliswanto, Eko Nur Sundahri, Sundahri Susanto, Arnis Budi Thohirin, Ahmad Nazmi Thongiratama, Michael Anthony Tri Agus Siswoyo Tri Handoyo TRI HANDOYO Tri Ratnasari Tri Rini Kusparwanti, Tri Rini Tri Widyastuti Ubaidillah, Mohammad Vega Kartika Sari Veronenci Yuliarbi Farlisa Wahyu Indra Duwi Fanata Widya Kristiyanti Putri Widya Kristiyanti Putri Wildan Muhlison, Wildan Wulanjari, Distiana Yuli Witono Zahro, Fauziatuz