Infection by the parasitic worm Anisakis sp. can cause anisakiasis in humans and is an important food safety concern, particularly in mackerel tuna (Euthynnus affinis). This study aimed to identify the morphology of Anisakis sp. larvae in mackerel tuna obtained from the Fish Auction Center (TPI) in Kupang, East Nusa Tenggara, using a Scanning Electron Microscope (SEM). Examination was conducted on internal organs (liver, intestine, and gonads), and the larvae found were observed using SEM. The results revealed the presence of Anisakis sp. larvae characterized by typical morphological features, including a boring tooth and papillae in the anterior part, as well as a mucron in the posterior part. These findings confirm Anisakis sp. infection in mackerel tuna and provide detailed morphological data useful for species identification and zoonosis prevention through marine fish quality monitoring.
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