Purpose: This study aimed to evaluate the differences in nutritional value between whole BSF larvae and defatted BSF larvae through chemical analysis and in vitro rumen fermentation trials. Methods: Four treatments were tested: whole BSF larvae (T1), mechanically defatted BSF larvae (T2), chemically defatted BSF larvae (T3), and soybean meal as a control (T4). Proximate analysis and Van Soest fiber fraction methods were performed. Results: Defatting improved dry matter and organic matter digestibility as well as gas production compared with whole BSF larvae. Ammonia concentration and VFA showed no significant differences among treatments. Conclusion: Fat reduction in BSF larvae enhanced their quality in in vitro rumen fermentation, indicating their potential as an alternative feed ingredient for ruminant livestock.
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