Jurnal Multidisipliner Kapalamada
Vol. 4 No. 04 (2025): JURNAL MULTIDISIPLINER KAPALAMADA

ANTIOXIDANT ACTIVITY EVALUATION OF CATFISH (CLARIAS SP.) COLLAGEN EXTRACT USING DPPH AND ABTS METHODS

Hiola, Faramita (Unknown)
Zuriati Uno, Wiwit (Unknown)
Sy. Pakaya, Mahdalena (Unknown)
Hutuba, Ariani (Unknown)

Article Info

Publish Date
30 Dec 2025

Abstract

This study aimed to evaluate the antioxidant activity of collagen extracted from catfish (Clarias sp.) using DPPH and ABTS assays. The research procedures included sample preparation, collagen extraction using an acid-based method, and antioxidant analysis at concentrations ranging from 20–100 ppm. The degree of hydrolysis (DH) was measured to determine the effectiveness of Alkalase in producing bioactive peptides. The results showed that higher enzyme concentrations led to increased DH, with the highest value of 62.42% obtained at 3% Alkalase. Antioxidant activity increased with sample concentration, with maximum inhibition values of 78.57% (DPPH) and 85.00% (ABTS) at 100 ppm. The ABTS assay exhibited higher sensitivity than DPPH. These findings indicate that catfish collagen has strong potential as a safe and natural antioxidant source suitable for further development in food, pharmaceutical, and cosmetic applications.

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Journal Info
Jurnal Multidisipliner Kapalamada
Website

Abbrev

Kapalamada

Publisher

Pusat Studi Ekonomi Publikasi Ilmiah dan Pengembangan SDM

Subject

Religion Agriculture, Biological Sciences & Forestry Humanities Economics, Econometrics & Finance Education Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Social Sciences Other

Description

KAPALAMADA JURNAL MULTIDISIPLINER is a quarterly journal that is published in Maret, Juni, September, and Desember. KAPALAMADA JURNAL MULTIDISIPLINER seeks Review articles, Case reports and original contributions from all areas of: Social Science and Humanities; Life Sciences; Health Science; ...