Biofarmasetikal Tropis (The Tropical Journal of Biopharmaceutical)
Vol. 8 No. 2 (2025): OKTOBER 2025

Analysis Of Rhodamine B Dye Content In Lipstick At Beriman Tomohon Market

Friska M. Montolalu (Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Christian University of Indonesia in Tomohon)
Christien Kiroh (Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Christian University of Indonesia in Tomohon)
Wilmar Maarisit (Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Christian University of Indonesia in Tomohon)
Jabes W. Kanter (Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Christian University of Indonesia in Tomohon)
Andry Natanel (Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Christian University of Indonesia in Tomohon)
Amal R. Ginting (Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Christian University of Indonesia in Tomohon)
Rachmat Santoso (Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Christian University of Indonesia in Tomohon)



Article Info

Publish Date
30 Oct 2025

Abstract

The use of cosmetics such as lipstick aims to improve the natural appearance of the lips and induce discoloration. A problem that is often worrying about today is the presence of prohibited dyes in lipstick preparations' main components. Rhodamine B is a synthetic dye prohibited in cosmetics, especially lipsticks. This research aims to analyze the Rhodamine B dye in lipsticks that do not have a distribution permit from BPOM circulating in the Beriman Tomohon market.The research method was Thin Layer Chromatography (TLC), followed by wavelength measurement on UV-Vis Spectrophotometry. The research population was all red lipstick preparations and 10 (ten) different lipsticks as a sample.Data analysis in this research is to find the RF value obtained from TLC then make a graph of the equation y = bx + a then look for the x value to measure the Rhodamine B content in lipstick. The results of this research were obtained from 1 (one) sample of lipstick label L(I) positive containing Rhodamine B. Visually, the lipstick of the L(I) label has a pink color and fluorescence orange when irradiated with 366nm UV, Rhodamine B has an Rf value of 0.85. In contrast, the L(I) sample has an Rf value of 0.84. Rhodamine B has a wavelength of 544nm, and the lipstick sample L(I) has an absorbance of 0.362 which has a Rhodamine B content of 0.4115%.

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Journal Info

Abbrev

jbt

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Chemistry Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

Description

Jurnal Biofarmasetikal Tropis (The Tropical Journal of Biopharmaceutical) is an Nasional journal presenting original research in Pharmacology and Toxicology, Pharmaceutical Chemistry, Drug Discovery, Pharmacokinetics, Pharmaceutical Biology, Herbal Medicines, Pharmaceutics, Pharmaceutical ...