ANTI-MELANOGENIC PROPERTIES OF CELERY LEAF EXTRACT IN HYPERPIGMENTATION INDUCED BY AZIDOTHYMIDINE

Dearni Damanik
Jekson M. Siahaan
Endy Juli Anto
Syafruddin Ilyas
Hadyanto L

Multidiciplinary Output Research for Actual and International Issue (Morfai Journal)
Vol. 6 No. 3 (2026): Multidiciplinary Output Research For Actual and International Issue

The purpose of this study is to examine the impact of ethanolic extract form Celery leaves (Apium graveolens L.) on hyperpigmented Wistar rats (Rattus norvegicus sp.) induced by Azidothymidine (AZT). Celery leaves are known to contain bioactive compounds which may serve as potent anti-inflammatory and antioxidant agents. About 30 male Wistar rats were divided into five groups, including a positive control, a negative control, and groups treated with various doses (1%, 3% and 5%) of celery leaf extract. The study examined inflammation indicators such as MDA, TNF-α, and IL-6. Celery leaf extract significantly (P<0.001) reduced MDA, TNF-α, and IL-6 levels in rats, demonstrating its capacity to attenuate inflammation and oxidative stress produced by AZT. With the increase in celery leaf extract concentration from 1% to 5%, MDA, IL-6 and TNF-α levels decreased from 42.083 ± 3.49 to 28.277 ± 10.27 nmol/ml, 14.588 ± 3.38 to 4.539 ± 1.40 nmol/ml and 667.614 ± 143.41 to 326.920 ± 62.72 nmol/ml respectively. Histopathological examinations demonstrated improvements in skin tissues damaged by inflammation. The results indicate that an ethanol extract of celery leaves has the potential to be an efficient anti-inflammatory drug, notably in lowering AZT-induced inflammation and hyperpigmentation. Further research is required to explore its clinical applicability in controlling inflammatory diseases in human.