Clove Essential Oil (CEO) has extensive benefits for health, cosmetics, and food. Low extraction yield (<1%) causes relatively high production costs. This becomes an opportunity for deliberate adulteration to gain greater business profits. This study developed a combination of UV-Vis spectrophotometry with chemometrics to separate pure CEO and adulterated CEO. The adulterants used included Corn Oil (CO) and Olive Oil (OO) at several adulteration levels to see their spectrum and absorption profiles using UV-Vis spectrophotometry. The data were analyzed using PCA and HCA chemometric techniques through R Studio 4.3.3 software. The results of UV-Vis spectrophotometry measurements on CEO adulteration showed similarities in the spectrum profiles of pure CEO and adulterated CEO. Data analysis with PCA can separate pure CEO from adultered CEO with a percentage of cumulative variance of the resulting spectrum of 98.2%. The more similar the composition of a particular oil in a sample, the closer the position of the sample to the oil on the score plot of PCA. HCA analysis can cluster pure CEO separately from adultered CEO. Cluster I in the dendogram consists of samples with large CO concentrations, while samples with large CEO and OO concentrations will be included in Cluster II. The combination of UV-Vis spectrophotometry and chemometrics can be used as a method to separate pure CEO from adultered CEO.
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