Hepatoprotective Effects of Milkfish (Chanos chanos) Oil and Silymarin Against Isoniazid–Rifampicin-Induced Liver Injury in Rats

Heru Sasongko (Department of Pharmacy, Vocational School, Sebelas Maret University, Jl. Ir. Sutami 36A Surakarta, Central Java)
Mikael Hovhanness (Department of Pharmacy, Vocational School, Sebelas Maret University, Jl. Ir. Sutami 36A Surakarta, Central Java)
Arinda Handiyah Sawitri (Department of Pharmacy, Vocational School, Sebelas Maret University, Jl. Ir. Sutami 36A Surakarta, Central Java)
Aulia Hanundita Maharani (Department of Pharmacy, Vocational School, Sebelas Maret University, Jl. Ir. Sutami 36A Surakarta, Central Java)
Delia Putri Hedianti (Department of Pharmacy, Vocational School, Sebelas Maret University, Jl. Ir. Sutami 36A Surakarta, Central Java)
Josua Arianto Hutasoit (Department of Pharmacy, Vocational School, Sebelas Maret University, Jl. Ir. Sutami 36A Surakarta, Central Java)
Lathyfa Asyraq (Department of Pharmacy, Vocational School, Sebelas Maret University, Jl. Ir. Sutami 36A Surakarta, Central Java)
Listiyana Ika Safitri (Department of Pharmacy, Vocational School, Sebelas Maret University, Jl. Ir. Sutami 36A Surakarta, Central Java)
Lois Elda Zai (Department of Pharmacy, Vocational School, Sebelas Maret University, Jl. Ir. Sutami 36A Surakarta, Central Java)
Sutarno Sutarno (Department of Biology, Faculty of Mathematics and Natural Science, Sebelas Maret University, Jl. Ir Sutami No.36A Surakarta, Central Java)

Majalah Obat Tradisional
Vol 31, No 1 (2026)

The objectives of the present research were to assess the hepatoprotective activities of milkfish oil (MFO) and silymarin (SL) against rifampicin (RFP) and isoniazid (INH) induced hepatotoxicity. Rats were divided into seven groups: normal control, negative control (INH+RFP), silymarin alone (50 mg/kg BW/day), low-dose MFO (MFO-L), high-dose MFO (MFO-H), low-dose combination (SL+MFO-L), and high-dose combination (SL+MFO-H). Rats receiving RFP and INH showed raised liver enzymes and typical signs of hepatotoxicity. Analyzed parameters comprised proinflammatory mediators (tumor necrosis factor-alpha and interleukin-6), antioxidant markers (catalase, glutathione, malondialdehyde, and superoxide dismutase), cytochrome P450, total protein, aspartate aminotransferase (AST), alanine aminotransferase (ALT), albumin and bilirubin. Treatment with a combination of MFO plus SL remarkably decreased hepatic enzyme activities, oxidative stress and inflammation, and suggested a prevention effect against the drug-induce liver injury.