PharmaCine: Journal of Pharmacy, Medical and Health Science
Vol. 7 No. 1 (2026): PharmaCine: Journal of Pharmacy, Medical and Health Science

Analisis Kadar Nitrit pada Daun Katuk dan Daun Pegagan dengan Pereaksi Griess: Analysis of Nitrite Levels in Katuk Leaves and Pegagan Leaves with Griess Reagent

Emma Emawati (Kelompok Keilmuan Analasis Farmasi dan Kimia Medisinal, Fakultas Farmasi, Universitas Bhakti Kencana)
Egy Rizky Maulana (Kelompok Keilmuan Analasis Farmasi dan Kimia Medisinal, Fakultas Farmasi, Universitas Bhakti Kencana)
Fauzan Zein Muttaqin (Kelompok Keilmuan Analasis Farmasi dan Kimia Medisinal, Fakultas Farmasi, Universitas Bhakti Kencana)
Lilis Silviana (Fakultas Farmasi, Universitas YPIB Majalengka)
Reza Pratama (Kelompok Keilmuan Farmasetika dan Teknologi Farmasi, Fakultas Farmasi, Universitas Bhakti Kencana)
Abdul Mulki Irpani (Kelompok Keilmuan Analasis Farmasi dan Kimia Medisinal, Fakultas Farmasi, Universitas Bhakti Kencana)



Article Info

Publish Date
31 Mar 2026

Abstract

Vegetables are an important source of nutrition for humans. Katuk (Sauropus androgynus) and pegagan (Centella asiatica) are vegetables rich in nutrients and fibre, which are beneficial for the body. In addition to their many health benefits, these vegetables contain compounds that can be harmful to the body, such as nitrite (NO₂⁻). The harmful effect caused by nitrite is methemoglobinemia. This study aims to analyze the nitrite levels contained in katuk (Sauropus androgynus) and pegagan vegetables using a visible spectrophotometric method using the Griess reagent based on the diazotization reaction principle. Before the analysis of nitrite levels, method validation was carried out, including linearity, accuracy, and precision tests. From the results of the linearity test, the linear regression line equation was obtained, namely, y = 0.0551x + 0.153, r = 0.9977, BD = 0.5647 μ,g / mL, BK = 1.8824 μg / mL. The results of the similarity test obtained the coefficient of variation of the first day, 0.88%, the second 1.12%, and the third day, 1.26%. While the percentage of nitrate recovery at concentrations of 3, 6, and 9 μg / ml with the standard addition method was obtained, respectively, 116, 88, and 90%. The nitrite content in katuk yielded an average of 0,1684 mg/g, and in pegagan was 0,4218 mg/g.

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Journal Info

Abbrev

pharmac

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

Description

PharmaCine: Journal of Pharmacy, Medical, and Health Sciences is a peer-reviewed journal dedicated to scientific publications in the fields of pharmacy, medicine, and health sciences. PharmaCine is published biannually, in March and September, by the Undergraduate Pharmacy Program, Faculty of Health ...