<![CDATA[VCO is a vegetable oil with many health and cosmetic benefits, but it is relatively more expensive than other vegetable oils, making it vulnerable to adulteration. UV-Vis spectrophotometry, combined with chemometric techniques, was used to distinguish VCO from other oils. Soybean oil (SO) and sunflower oil (SFO) are the main adulterants of VCO. This study aimed to evaluate the ability of UV-Vis spectrophotometry combined with chemometrics to differentiate VCO from SO and SFO. Samples were measured for absorbance at wavelengths from 200 to 400 nm. The UV-Vis spectral data were further analyzed using chemometric methods, PCA, and HCA, to classify the samples. The PCA model successfully differentiated VCO, SO, and SFO in RStudio 4.4.2, with variance explained by PC1 (81.3%) and PC2 (17.1%). VCO samples are clearly separated from other samples, while SO and SFO appear close to each other due to their similar physicochemical properties. Mixed oil samples were grouped by composition. HCA analysis also showed a grouping pattern consistent with PCA results. Therefore, UV-Vis spectrophotometry, combined with chemometric techniques, can effectively distinguish VCO from SO and SFO.]]>
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