<![CDATA[Science literacy among Indonesian elementary school students remains relatively low, as reflected in difficulties explaining scientific phenomena, interpreting data, and applying scientific concepts to everyday situations. Artificial Intelligence (AI)-based learning media offer an interactive, student-centered approach to address this issue. This study aimed to examine the effect of AI Assistant-based learning media on the science literacy of fourth-grade students in learning changes in the states of matter. A quantitative approach with a quasi-experimental method and Nonequivalent Control Group Design was employed. The sample consisted of 32 students assigned to an experimental class and a control class. Data were collected using pretest and posttest science literacy assessments and analyzed through N-Gain, Wilcoxon Signed Rank Test, Mann–Whitney U Test, and Cohen's d Effect Size. The results showed a significant difference in science literacy improvement between the two groups (Asymp. Sig. = 0.017, p < 0.05). The experimental class achieved a higher average N-Gain (0.64) than the control class (0.48), while Cohen's d value of 0.9226 indicated a large effect. Therefore, AI Assistant-based learning media effectively improved students' science literacy. These findings suggest that AI Assistant can support the implementation of the Merdeka Curriculum and promote innovative science learning in elementary schools.]]>
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