Biodiversitas Journal of Biological Diversity
Vol. 20 No. 7 (2019)

Analysis of IGF-1 gene in ayam ketawa (Gallus gallus domesticus) with dangdut and slow type vocal characteristics

ABINAWANTO ABINAWANTO (Biology Postdoctoral Program, Faculty of Mathematics and Natural Sciences, Universitas Indonesia. Jl. Lingkar UI, E Building UI Campus, Depok 16242, West Java, Indonesia)
ALFI SOPHIAN (Biology Postdoctoral Program, Faculty of Mathematics and Natural Sciences, Universitas Indonesia. Jl. Lingkar UI, E Building UI Campus, Depok 16242, West Java, Indonesia)
RETNO LESTARI (Biology Postdoctoral Program, Faculty of Mathematics and Natural Sciences, Universitas Indonesia. Jl. Lingkar UI, E Building UI Campus, Depok 16242, West Java, Indonesia)
ANOM BOWOLAKSONO (Biology Postdoctoral Program, Faculty of Mathematics and Natural Sciences, Universitas Indonesia. Jl. Lingkar UI, E Building UI Campus, Depok 16242, West Java, Indonesia)
PIPIH SUNINGSIH EFENDI (Genetics Laboratory, Faculty of Animal Husbandry, Institut Pertanian Bogor. Jl. Raya Dramaga, Bogor 16680, West Java, Indonesia)
RUDI AFNAN (Genetics Laboratory, Faculty of Animal Husbandry, Institut Pertanian Bogor. Jl. Raya Dramaga, Bogor 16680, West Java, Indonesia)



Article Info

Publish Date
03 Jul 2019

Abstract

Abstract. Abinawanto, Sophian A, Lestari R, Bowolaksono A, Efendi PS, Afnan R. 2019. Analysis of IGF-1 gene in ayam ketawa (Gallus gallus domesticus) with dangdut and slow type vocal characteristics. Biodiversitas 20: 2004-2010. Insulin-like growth factor 1 (IGF-1) is defined as an essential gene for growth. Therefore, this research aims to identify the polymorphism of the IGF-1 gene from ayam ketawa with the dangdut and slow type vocal characteristics. Twenty male chickens were selected as an animal model. The sample consisted of 10 chickens with the dangdut type and 10 chickens with the slow type. The chickens were all obtained from five areas of the Pinrang district. The RFLP and HRM methods were selected to be used in this research. Results showed that the IGF-1 gene was 622 bp in length, while polymorphism analysis showed that the dangdut and slow type are homozygotic. The unrestricted DNA fragment indicated it after reaction of the restriction enzyme Pst1. Moreover, it confirmed that ayam ketawa with the dangdut or slow type had a similar sequence.

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Journal Info

Abbrev

biodiv

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was ...