Biodiversitas Journal of Biological Diversity
Vol. 25 No. 2 (2024)

First report of Pyricularia oryzae the cause of blast disease in upland rice, in Lombok, West Nusa Tenggara

IRNA IL SANURIZA (Doctoral Program of Biology, Faculty of Mathematics and Natural Sciences, Universitas Udayana. Kampus Bukit Jimbaran, Denpasar 80361, Bali, Indonesia)
DEWA NGURAH SUPRAPTA (Doctoral Program of Agriculture Science, Faculty of Agriculture, Universitas Udayana. Jl. Raya Kampus UNUD, Bukit Jimbaran, Kuta Selatan, Badung 80361, Bali, Indonesia)
RETNO KAWURI (Doctoral Program of Biology, Faculty of Mathematics and Natural Sciences, Universitas Udayana. Kampus Bukit Jimbaran, Denpasar 80361, Bali, Indonesia)
NI LUH SURIANI (Doctoral Program of Biology, Faculty of Mathematics and Natural Sciences, Universitas Udayana. Kampus Bukit Jimbaran, Denpasar 80361, Bali, Indonesia)
I MADE SUDANTHA (Department of Agrotechnology, Faculty of Agriculture, Universitas Mataram. Jl. Majapahit No. 62, Mataram 83115, West Nusa Tenggara, Indonesia)
IRFAN JAYADI (Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Nahdlatul Wathan Mataram. Jl. Kaktus No. 1-3 Gomong, Mataram 83126, West Nusa Tenggara, Indonesia)
KHAERUL IHWAN (Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Nahdlatul Wathan Mataram. Jl. Kaktus No. 1-3 Gomong, Mataram 83126, West Nusa Tenggara, Indonesia)



Article Info

Publish Date
27 Feb 2024

Abstract

Rice (Oryza sativa L.) is a maincrop cultivated by farmers in Indonesia. The demand for rice has increased, but rice production has decreased due to attacks by plant pests, including pathogen blast disease. This research aims to (i) determine the symptoms of blast disease in upland rice in Lombok, (ii) determine the incidence of blast disease in upland rice in Lombok, (iii) and identify the pathogen that causes blast disease in upland rice in Lombok. The research was conducted in Lombok, West Nusa Tenggara. A sampling of rice crop tissue infected by blast disease was taken from 50  dryland locations in Lombok West Nusa Tenggara. From each point, three samples of rice crops with blast symptoms were taken to obtain 150 samples. Samples are then isolated and identified at the macroscopic, microscopic, and molecular levels. The study results showed that the macroscopic characteristics of rice leaves were rhombus-shaped and brown, and the pathogen produced white mycelium with a fine structure. In contrast, microscopic characteristics showed that the shape of the hyphae was septate, the structure of conidiophore with autophagic cell death, long pyriform microconidia, and long pyriform macroconidia. Based on 18S rRNA gene analysis, the Blast Lombok isolate had a DNA fragment of ±550bp. Based on these results, it can be concluded that the cause of blast disease in upland rice in Lombok, West Nusa Tenggara, is P. oryzae.

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Journal Info

Abbrev

biodiv

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was ...