Isolation and characterization of plant growth-promoting bacteria from medicinal plants Java cardamom (Amomum compactum) and bitter ginger (Zingiber zerumbet)

ALMANDO GERALDI (Department of Biology, Faculty of Science and Technology, Universitas Airlangga. Jl. Dr. Ir. H. Soekarno, Surabaya 60115, East Java, Indonesia)
CHRISTOPHER CLEMENT (Department of Biology, Faculty of Science and Technology, Universitas Airlangga. Jl. Dr. Ir. H. Soekarno, Surabaya 60115, East Java, Indonesia)
MAYA DEWI PERTIWI (Department of Biology, Faculty of Science and Technology, Universitas Airlangga. Jl. Dr. Ir. H. Soekarno, Surabaya 60115, East Java, Indonesia)
YESYI LESTARI (Department of Biology, Faculty of Science and Technology, Universitas Airlangga. Jl. Dr. Ir. H. Soekarno, Surabaya 60115, East Java, Indonesia)
DHAMMARUCI HEMADHIRA PARINNATA (Department of Biology, Faculty of Science and Technology, Universitas Airlangga. Jl. Dr. Ir. H. Soekarno, Surabaya 60115, East Java, Indonesia)
RIFAID NUR ARSAD (Department of Biology, Faculty of Science and Technology, Universitas Airlangga. Jl. Dr. Ir. H. Soekarno, Surabaya 60115, East Java, Indonesia)
RAMA ALI SADIKIN (Department of Biology, Faculty of Science and Technology, Universitas Airlangga. Jl. Dr. Ir. H. Soekarno, Surabaya 60115, East Java, Indonesia)
ARIF LUQMAN (Institute for Molecular Infection Biology, Julius Maximilians University of Wuerzburg. Wuerzburg, Germany)
HERI SANTOSO (Generasi Biologi Indonesia (Genbinesia) Foundation. Jl. Swadaya Barat No. 4, Gresik 61171, East Java, Indonesia)
ALFINDA NOVI KRISTANTI (Department of Chemistry, Faculty of Science and Technology, Universitas Airlangga. Jl. Dr. Ir. H. Soekarno, Surabaya 60115, East Java, Indonesia)
YOSEPHINE SRI WULAN MANUHARA (Department of Biology, Faculty of Science and Technology, Universitas Airlangga. Jl. Dr. Ir. H. Soekarno, Surabaya 60115, East Java, Indonesia)
ANJAR TRI WIBOWO (Department of Biology, Faculty of Science and Technology, Universitas Airlangga. Jl. Dr. Ir. H. Soekarno, Surabaya 60115, East Java, Indonesia)

Biodiversitas Journal of Biological Diversity
Vol. 25 No. 6 (2024)

Abstract. Geraldi A, Clement C, Pertiwi MD, Lestari Y, Parinnata DH, Arsad RN, Sadikin RA, Luqman A, Santoso H, Kristanti AN, Manuhara YSW, Wibowo AT. 2024. Isolation and characterization of plant growth-promoting bacteria from medicinal plants Java cardamom (Amomum compactum) and bitter ginger (Zingiber zerumbet). Biodiversitas 25: 2556-2564. Java cardamom (Amomum compactum) and bitter ginger (Zingiber zerumbet) are valuable medicinal plants native to Indonesia with significant economic and pharmacological importance. While their pharmacological properties have been extensively studied, little is known about the role of their endophytic bacterial communities in influencing plant growth and development. Understanding the interactions between these plants and their associated bacteria could provide insights into novel strategies to enhance their cultivation. This study investigates the potential of endophytic bacteria associated with A. compactum and Z. zerumbet in promoting plant growth. Samples were collected from leaf, rhizome, and rhizosphere soil, followed by isolation and characterization of bacterial strains. Molecular identification revealed the presence of Methylobacterium aquaticum, Paenibacillus tyrfis, Priestia megaterium strain 1, Priestia aryabhattai, and Microbacterium arthrosphaerae among the isolated strains from A. compactum, while strains isolated from Z. zerumbet included Enterobacter mori, Pr. megaterium strain 2, and Pr. megaterium strain 3. Those results demonstrated that Pr. megaterium strains exhibited strong phosphate solubilization, nitrogen fixation, and cellulolytic activity, while Pr. aryabhattai and Pa. tyrfis showed significant nitrogen fixation and cellulolytic potential. Additionally, M. aquaticum, Pa. tyrfis, and Pr. aryabhattai exhibited anti-phytopathogenic activity against Xanthomonas campestris. These findings not only highlight the diverse beneficial attributes of the isolated bacterial strains but also suggest their potential application as biofertilizers and biocontrol agents in agriculture, offering a promising avenue for sustainable farming practices.