The bacteria Propionibacterium acnes (PA) and Staphylococcus epidermidis (SE) cause acne vulgaris, an infection marked by inflammation in the sebaceous glands, as well as obstruction and accumulation of keratinous components. Kombucha contains antimicrobial chemicals, among other components. The active components' antibacterial properties should be strengthened by the addition of Nardus grass. Finding the most ideal concentration and the efficiency of Nardus grass kombucha in suppressing PA and SE bacteria were the goals of this investigation. The Kirby-Bauer antibacterial testing method was used to test Nardus grass kombucha against PA and SE bacteria after it had fermented for 14 days at concentrations of 70%, 80%, and 90%. ANOVA and SPSS software were used to analyze the data. According to the test results, the development of clean zones demonstrated that Nardus grass kombucha at all concentrations may stop bacterial growth. At 70%, 80%, and 90% concentrations, the inhibition zones' average diameters against PA bacteria were 16.60 mm, 18.53 mm, and 20.96 mm, respectively. At the same doses, the inhibition zones for SE bacteria were 15.40 mm, 16.30 mm, and 17.53 mm. The results of the study showed that nardus grass kombucha can inhibit PA and SE bacteria, with 90% being the ideal concentration.
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