This study aims to evaluate the psychometric quality of the questionnaire instrument used to measure material mastery and learning media preferences (Linear Text Summary vs. Concept Map) in the Human Anatomy and Physiology course on the Nervous System material. The research employed a quantitative approach with an instrument research method. The research subjects consisted of 28 students who had completed learning using both media in stages. The Material Mastery section instrument consisted of 10 statement items using a 4-point Likert scale, while the Direct Preference section consisted of 4 statement items using a 3-point choice scale. Data were analyzed using the Pearson Product Moment validity test, Cronbach's Alpha reliability test, and descriptive analysis. The results showed that all 10 items of the Material Mastery section were valid for both media (r_calculated > 0.374) with a very high combined reliability (α = 0.883). In contrast, the Direct Preference section had one invalid item (Pref 2: r = 0.322) and very low reliability (α = 0.237). The conclusion of this study is that the Material Mastery instrument is feasible to measure the perception of material mastery in Human Anatomy and Physiology, while the Direct Preference section cannot be treated as a unidimensional scale and should be analyzed item by item as independent categorical data.
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