<![CDATA[Seurukan fish (Osteochilus vittatus) is a freshwater fish of economic value whose supply still relies heavily on wild catches. In aquaculture, particularly during grading, transportation and research, anaesthetics are needed to reduce stress and to improve survival. Common synthetic anaesthetics may leave residues and harm the environment, so that safe natural alternatives are required. Clove oil (Syzygium aromaticum L.), which contains eugenol, is known to have anaesthetic and sedative properties in fish. This study aimed to determine the effect and the optimal dose of clove oil as a natural anaesthetic for seurukan juveniles. It was conducted in October 2025 at the Hatchery of the Faculty of Fisheries and Marine Science, Teuku Umar University, using a completely randomised design with four treatments and three replications: a control without clove oil (P0) and doses of 0.02 ml/L (P1), 0.04 ml/L (P2) and 0.06 ml/L (P3). The parameters observed were induction and sedative time, blood glucose, operculum opening frequency, survival and water quality. Clove oil had no significant effect on survival or blood glucose (P>0.05), but it lowered the operculum opening frequency and accelerated induction. A dose of 0.04 ml/L was the most effective, giving an optimal anaesthetic response, a good recovery time and the highest survival (96.67%).]]>
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