<![CDATA[Cardiovascular diseases such as pulmonary arterial hypertension can lead to increased workload on the right ventricle, which clinically manifests as peripheral edema. The exploration of herbal ingredients like butterfly pea flower (Clitoria ternatea L.) represents a preventive strategy due to its anti-angiogenic and cardioprotective activities. This study aimed to evaluate the cardioprotective effect of butterfly pea flower extract syrup in reducing pericardial edema in a zebrafish embryo model. Zebrafish embryos at 24 hours post-fertilization were exposed to butterfly pea flower syrup at various concentrations (1.56, 3.12, 6.25, 12.5, 25, and 50 µg/mL), along with a negative control (E3 medium) and a positive control (0.1% CHX) for 96 hours. The pericardial area was quantitatively analyzed using FishInspector software and computed using the Shoelace Formula in RStudio, followed by statistical analysis using SPSS. The results showed that the CHX group exhibited severe edema, with a pericardial area reaching 8,327.56 pixel². Concentrations of 50 µg/mL and 25 µg/mL of butterfly pea syrup induced increased fluid accumulation, potentially influenced by the osmolarity of the preparation. In contrast, concentrations 1.56 to 12.5 µg/mL maintained normal morphology, with pericardial area values ranging from 3,646.00 to 3,890.74 pixel², which were close to the negative control group (3,589.91 pixel²). Statistical analysis using ANOVA revealed a p-value of 0.448 (p > 0.05), indicating that the treatment groups exhibited a uniform cardioprotective effect without cardiotoxicity. Therefore, butterfly pea flower extract syrup at 5% concentration shows potential as a preventive therapeutic modality to reduce the risk of peripheral edema.]]>
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