This study aimed to synthesize cellulose nanocrystals from cassava stems (Manihot esculenta Crantz.) and evaluate their potential as a filler in biodegradable plastics. The method used includes cellulose isolation through alkalization and bleaching processes, followed by hydrolysis using 10% sulfuric acid (H₂SO₄) at 45 °C for 60 minutes. Characterization was carried out using FTIR to analyze functional groups, XRD to determine crystallinity, and TEM to observe morphology and particle size. The results showed that the obtained cellulose nanocrystals had a yield of 78.8%. FTIR analysis indicated that the main functional groups of cellulose were still detected, while XRD analysis showed a crystallinity index of 60.73%. TEM results revealed that the particle sizes ranged from 21.43–383.34 nm. In conclusion, cellulose nanocrystals from cassava stems have the potential to be used as a filler in biodegradable plastics due to their nanoscale size and crystalline structure, which can improve the properties of environmentally friendly materials. Keywords: Acid Hydrolysis, Cassava Stem, Cellulose Nanocrystals, Filler
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