<![CDATA[Acne is a common skin condition caused by excess oil production. Propionibacterium acnes is one of the bacteria involved in the development of acne. Long-term use of antibiotics to treat acne can cause side effects and bacterial resistance, making alternative treatments derived from natural sources necessary. Robusta coffee beans and green cincau leaves are known to contain secondary metabolites with potential antibacterial properties. This study aims to determine the antibacterial activity of a combination of Robusta coffee bean (Coffea canephora) and green cincau leaf (Cyclea barbata Miers) extracts against the growth of Propionibacterium acnes, as well as to identify significant differences in their antibacterial activity. This study employed a quantitative experimental method. Phytochemical screening was conducted for alkaloids, flavonoids, tannins, and saponins, while the antibacterial activity test used the disk diffusion method with three concentration variations of the extract combination: 12.5% : 15%, 25% : 30%, and 50% : 60%. Clindamycin was used as the positive control and sterile distilled water as the negative control. The results of the phytochemical screening showed that both extracts contained alkaloids, flavonoids, tannins, and saponins. The combination of Robusta coffee bean extract and green cincau leaf extract exhibited antibacterial activity, with average inhibition zone diameters of 4.49 mm, 6.12 mm, and 6.13 mm, respectively. Statistical analysis revealed significant differences in antibacterial activity with p-values <0.05. The study concluded that the combination of Robusta coffee bean extract and green cincau leaf extract is capable of inhibiting the growth of Propionibacterium acnes, exhibiting weak to moderate antibacterial activity.]]>
Copyrights © 2026
