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Evaluation of the Antibacterial Activity of a Combination of Robusta Coffee (Coffea Canephora) Seed Extract and Green Grass Jelly (Cyclea Barbata Miers) Leaf Extract Against Propionibacterium Acnes Ananda Putri Nabila; Wina Safutri; Riza Dwiningrum; Iga Mayola Pisacha
JURNAL KESEHATAN, SAINS, DAN TEKNOLOGI (JAKASAKTI) Vol. 5 No. 2 (2026): JURNAL KESEHATAN, SAINS, DAN TEKNOLOGI (JAKASAKTI)
Publisher : LPPM Universitas Dhyana Pura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36002/js.v5i2.5749

Abstract

Acne is a common skin condition caused by excess oil production. Propionibacterium acnes is one of the bacteria involved in the development of acne. Long-term use of antibiotics to treat acne can cause side effects and bacterial resistance, making alternative treatments derived from natural sources necessary. Robusta coffee beans and green cincau leaves are known to contain secondary metabolites with potential antibacterial properties. This study aims to determine the antibacterial activity of a combination of Robusta coffee bean (Coffea canephora) and green cincau leaf (Cyclea barbata Miers) extracts against the growth of Propionibacterium acnes, as well as to identify significant differences in their antibacterial activity. This study employed a quantitative experimental method. Phytochemical screening was conducted for alkaloids, flavonoids, tannins, and saponins, while the antibacterial activity test used the disk diffusion method with three concentration variations of the extract combination: 12.5% : 15%, 25% : 30%, and 50% : 60%. Clindamycin was used as the positive control and sterile distilled water as the negative control. The results of the phytochemical screening showed that both extracts contained alkaloids, flavonoids, tannins, and saponins. The combination of Robusta coffee bean extract and green cincau leaf extract exhibited antibacterial activity, with average inhibition zone diameters of 4.49 mm, 6.12 mm, and 6.13 mm, respectively. Statistical analysis revealed significant differences in antibacterial activity with p-values <0.05. The study concluded that the combination of Robusta coffee bean extract and green cincau leaf extract is capable of inhibiting the growth of Propionibacterium acnes, exhibiting weak to moderate antibacterial activity.
FORMULATION AND PHYSICAL EVALUATION OF DEODORANT CREAM PREPARATIONS BASED ON GREEN BETEL LEAF (Piper Betle L.) EXTRACT WITH THE ADDITION OF ALUM (Aluminiu Potassium Sulfat) Runia Safitri; Nadia Dwi Oktaviani; Annajim Daskar; Wina Safutri
JURNAL KESEHATAN, SAINS, DAN TEKNOLOGI (JAKASAKTI) Vol. 5 No. 2 (2026): JURNAL KESEHATAN, SAINS, DAN TEKNOLOGI (JAKASAKTI)
Publisher : LPPM Universitas Dhyana Pura

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Body odour is a common problem experienced by people in tropical climates due to increased sweat production and perspiration on the skin. The continuous use of synthetic deodorants can cause side effects, making it necessary to find safer, natural alternatives. Green betel leaves (Piper betle L.) are known to possess antibacterial activity as they contain alkaloids, flavonoids, saponins and tannins. Alum, meanwhile, acts as an antiperspirant and bacteriostatic agent. This study aims to determine the formulation and evaluate the physical properties of a deodorant cream based on green betel leaf extract with the addition of alum. Green betel leaf extract (Piper betle L.) was obtained via maceration using 96% ethanol as the solvent. The cream formulation was prepared in three variations of green betel leaf extract concentration, namely F1 (2%), F2 (3%) and F3 (4%), with the addition of 2% alum. The physical evaluations carried out included organoleptic testing, homogeneity testing, pH testing, spreadability testing, adhesion testing, viscosity testing, cream type testing, and formulation stability testing. The results of the study showed that F1, F2 and F3 were green in colour, had a characteristic odour, were semi-solid in consistency, homogeneous and were of the oil-in-water (O/W) emulsion type. The deodorant cream preparations in each formulation (F1, F2, F3) had pH, spreadability, adhesion and viscosity values that met the requirements for a good cream preparation, and demonstrated good physical stability test results, characterised by the absence of phase separation of the cream following a centrifugation test at 4000 rpm for 30 minutes. Based on the results of the physical evaluation of the deodorant cream formulation containing green betel leaf extract (Piper betle L.) with the addition of alum (Aluminiu Potassium Sulfat), it was found to meet the physical quality requirements and has the potential to be used as a natural deodorant alternative.