This study aimed to determine and analyze the effect of using poster-based learning media on students’ Biology learning outcomes in the Human Digestive System topic among Grade XI students of SMA Negeri 27 Kabupaten Tangerang. The background was rooted in students' low learning outcomes due to limited utilization of engaging visual media, causing abstract concepts to be difficult to grasp. The research employed a quantitative quasi-experimental design with a Nonequivalent Control Group Design. The population comprised all Grade XI students in the 2025/2026 academic year. Using purposive sampling, Class XI F5 was selected as the experimental class (n = 36) taught with poster media, while Class XI F3 served as the control class (n = 35) taught using conventional methods. Data were collected via a 26-item multiple-choice test validated for content and empirical accuracy (Cronbach's Alpha = 0.924). Descriptive analysis revealed that the experimental class achieved a mean posttest score of 94.88 (up from a pretest score of 62.96), whereas the control class achieved a mean posttest score of 75.92 (up from a pretest score of 61.60). Prerequisite tests confirmed normal distribution and variance homogeneity. The Independent Samples T-Test yielded a 2-tailed significance value of 0.000 < 0.05, rejecting H0 and accepting H1. It is concluded that poster-based learning media significantly enhances learning outcomes in the Human Digestive System topic.
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