<![CDATA[Needle Stick Injury (NSI) is a serious occupational hazard among healthcare workers due to the risk of transmitting bloodborne pathogens. A preliminary study at Puskesmas X found that 60% of healthcare workers had experienced NSI. This study aimed to analyze the relationship between work standards, skills, and training with the incidence of NSI among healthcare workers (nurses and midwives) at Puskesmas X, East Bolaang Mongondow Regency. An analytical observational study with a cross-sectional design was conducted. A total sampling technique was used, resulting in 37 respondents. Data were collected using a structured questionnaire and analyzed using statistical tests. The results showed majority of respondents were aged 26–34 years (67.6%), female (81.1%), and had experienced NSI (73.0%). Statistical analysis showed a significant association between work standards (p = 0.018), skills (p < 0.001), and training (p = 0.014) with NSI incidence. Poor work standards, inadequate clinical skills, and limited participation in training were strongly associated with higher rates of NSI. Community health centers are recommended to implement regular occupational safety training programs to improve the technical skills of healthcare workers.]]>
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