Biology, Medicine, & Natural Product Chemistry
Vol 15, No 2 (2026)

Effect of Fermentation Time and Sucrose Concentration on the Quality Characteristics of Refermented Dried Cocoa Beans (MCC02)

Analianasari Analianasari (Politeknik Negeri Lampung)
Giffary Pramafisi Soeherman (Politeknik Negeri Lampung)
Dayang Berliana (Politeknik Negeri Lampung)



Article Info

Publish Date
05 Nov 2026

Abstract

The quality of Indonesian cocoa beans in the international market remains relatively low due to suboptimal post-harvest handling. The fermentation process, typically carried out by farmers for 4–5 days in accordance with SNI standards, is often uncontrolled due to limited fermentation facilities, resulting in a high moisture content and an underdeveloped flavor. This study aimed to (1) analyze the quality of re-fermented cocoa beans based on SNI Cocoa Beans No. 2323-2008 under treatments involving different sucrose concentrations and fermentation times, and (2) analyze the fermentation index values of re-fermented cocoa beans under the same treatments. The study employed a factorial design with two factors: sucrose concentration (0%, 2.5%, 5%, 7.5%, and 10%) and fermentation duration (1, 2, and 3 days). Re-fermentation was carried out on farmers’ dried cocoa beans using fermentation bags with added sucrose as a process catalyst. The parameters observed included compliance with SNI Cocoa Beans No. 2323-2008 and fermentation index values measured using a spectrophotometer. The results showed that fermentation time had a major effect on the physical properties of cocoa beans (moisture content and weight), whilst sucrose concentration had a greater influence on biochemical aspects (pH and fermentation index). The optimal combination was achieved with a 2-day fermentation period and 2.5% sucrose (A2 × B1), yielding bean quality closest to ideal conditions. Re-fermentation was found to improve cocoa bean quality compared to unrefmented beans, suggesting its potential as a quality-enhancement solution at the farmer level.

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Journal Info

Abbrev

BIOMEDICH

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology Public Health

Description

BIOLOGY, MEDICINE, & NATURAL PRODUCT CHEMISTRY, this journal is published to attract and disseminate innovative and expert findings in the fields of plant, animal, and microorganism secondary metabolite, and also the effect of natural product on biological system as a reference source for ...