<![CDATA[Dengue Hemorrhagic Fever (DHF), transmitted by Aedes aegypti mosquitoes, remains a public health problem, while the use of synthetic insecticides carries the risk of resistance and environmental pollution. This research aimed to identify the secondary metabolites in lime (Citrus aurantifolia) and kaffir lime (Citrus hystrix) leaf extracts, to determine the most effective extract combination for producing the highest mortality of third-instar Aedes aegypti larvae, and to observe the resulting morphological changes. The research employed a Completely Randomized Design (CRD) with four treatments: a control (distilled water), F1 (10 mL of lime leaf extract + 10 mL of kaffir lime leaf extract), F2 (20 mL of lime leaf extract + 20 mL of kaffir lime leaf extract), and F3 (30 mL of lime leaf extract + 30 mL of kaffir lime leaf extract). Larval mortality percentages were analyzed using the non-parametric Kruskal-Wallis test, followed by Dunn’s post hoc test. Phytochemical screening revealed the presence of alkaloids, flavonoids, tannins, and saponins. The results showed that the treatment containing 30 mL of lime leaf extract + 30 mL of kaffir lime leaf extract was the most effective, with a mean mortality of 95.40%. Morphological observations showed that increasing extract concentrations caused structural damage to Aedes aegypti larvae.]]>
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