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EDITOR IN CHIEF Gerald H. Tamuntuan, Universitas Sam Ratulangi, Indonesia MANAGING EDITOR Verna A. Suoth, Universitas Sam Ratulangi, Indonesia BOARD OF EDITOR Audy Wuntu, Fakultas MIPA Univesitas Sam Ratulangi BOARD OF EDITOR Nio Song Ai, Universitas Sam Ratulangi, Indonesia BOARD OF EDITOR Nelson Naingolang, Universitas Sam Ratulangi, Indonesia
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Kota manado,
Sulawesi utara
INDONESIA
Jurnal MIPA
Published by Universitas Sam Ratulangi
ISSN : -     EISSN : 23023899     DOI : https://doi.org/10.35799/jmuo.10.2.2021.33592
Core Subject : Science, Education,
Biochemistry, Genetics & Molecular Biology Chemistry Mathematics Physics
Jurnal MIPA menjadi sarana publikasi bagi akademisi dan peneliti. Jurnal MIPA mempublikasikan artikel hasil penelitian di bidang : Matematika Fisika Biologi Kimia
Arjuna Subject : Umum - Umum
Articles 16 Documents
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Search results for , issue "Vol 3, No 2 (2014)" : 16 Documents clear
Aktivitas Penangkal Radikal Hidroksil Fraksi Flavonoid dari Limbah Tongkol Jagung pada Tikus Wistar Maanari, Chaleb P.; Suryanto, Edi; Pontoh, Julius
Jurnal MIPA Vol 3, No 2 (2014)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jm.3.2.2014.5990

Abstract

Penelitian telah dilakukan untuk menentukan kemampuan menangkal radikal hidroksil pada homogenat hati, jantung dan otak tikus wistar dari fraksi flavonoid limbah tongkol jagung. Penelitian dimulai dengan mengekstraksi serbuk tongkol jagung yang sudah dikering anginkan menggunakan cara refluks selama dua jam dengan pelarut etanol 80%. Ekstrak kemudian dipartisi berturut-turut menggunakan petroleum eter, etil asetat, n-butanol dan air, selanjutnya ditentukan kandungan total flavonoid serta aktivitas penangkal radikal hidroksil pada homogenat jaringan hati, jantung dan otak tikus wistar. Hasilnya menunjukkan ekstrak etil asetat yang memiliki kandungan total flavonoid yang paling tinggi yaitu 41,926 mg/kg ekstrak, serta kemampuan menangkal radikal hidroksil pada homogenat jaringan hati, jantung dan otak tikus wistar sebesar 90,964%; 86,875% dan 68,235%.This research has been conducted to determine radical hydroxyl scavenging ability on a wistar rat tissue homogenate of liver, heart and brain from corn cob waste extract. Research started with with extracting the powder of corn cob which had been air-dried using reflux method during two hours with 80% ethanol solvent. Then the extract successively partitioned with petroleum ether, ethyl acetate, n-buthanol and water, next determined the flavonoid total content with activities of hydroxyl radical on a wistar rat tissue homogenate. The result showed ethyl acetate extract which has good result for flavonoid total content that is 41,926 mg/kg extract, with ability of free radical scavenger on a rat tissue homogenate of liver, heart and brain as much as 90,964%; 86,875% and 68,235%.
Fotodegradasi Zat Warna Metanil Yellow Menggunakan Fotokatalis TiO2-Karbon Aktif Togas, Christmas; Wuntu, Audy D.; Koleangan, Harry S. J.
Jurnal MIPA Vol 3, No 2 (2014)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jm.3.2.2014.5857

Abstract

Telah dilakukan penelitian untuk mempelajari pengaruh penambahan karbon aktif (KA) pada fotokatalis TiO2 serta aktivitas fotokatalitiknya dalam proses fotodegradasi zat warna metanil yellow. Penelitian diawali dengan pembuatan KA dari tempurung kelapa, kemudian dilakukan modifikasi fotokatalis TiO2–KA. Eksperiman fotodegradasi metanil yellow oleh fotokatalis TiO2–KA dilakukan dengan perbandingan berat TiO2:KA sebesar 9,9:0,1 dan 9,5:0,5 dan konsentrasi metanil yellow 2–50 ppm, serta variasi waktu penyinaran sinar UV selama 1, 2, 3, 4, 5, 19 dan 20 jam. Hasil penelitian menunjukkan bahwa persentase proses fotodegradasi tertinggi diperoleh pada perbandingan berat TiO2:KA (9,9:0,1) dan aktivitas fotodegradasi semakin meningkat seiring dengan bertambahnya waktu penyinaran.A research had been conducted to study the effect of the addition of activated carbon (AC) on TiO2 photocatalyst and its photocatalytic activity in photodegradation process of metanil yellow dye. The research was performed through the preparation of activated carbon from coconut shell and modification of photocatalyst TiO2–AC. Experiment of metanil yellow photodegradation by photocatalyst TiO2–AC was performed at the weight ratio of TiO2:AC of 9,9:0,1 and 9,5:0,5 with the concentrations of metanil yellow of 2–50 ppm, and time variations of UV rays irradiation of 1, 2, 3, 4, 5, 19 and 20 hours. The results showed that the highest percentage of photodegradation process obtained at the weight ratio of TiO2:AC of 9,9:0,1 and the photodegradation activity was increased along with increasing irradiation time
Barcode DNA Tanaman Leilem (Clerodendrum minahassae L.) Berdasarkan Gen matK Kalangi, Cindy; Kamu, Vanda S.; Kumaunang, Maureen
Jurnal MIPA Vol 3, No 2 (2014)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jm.3.2.2014.5861

Abstract

Gen matK merupakan gen pengkode protein maturaseK yang terdapat pada kloroplas tumbuhan. Penelitian ini bertujuan untuk menentukan urutan nukleotida dari barcode DNA tanaman leilem (Clerodendrum minahassae L.) berdasarkan gen matK. Prosedur penelitian yang dilakukan meliputi: isolasi DNA total tanaman leilem, amplifikasi gen matK melalui PCR, sekuensing hasil PCR, serta penentuan barcode DNA leilem. Isolasi DNA total dari tanaman leilem telah dilakukan berdasarkan prosedur manual dari InnuPrep Plant DNA Kit yang dimodifikasi dengan menghasilkan larutan berwarna hijau kekuningan yang menunjukkan adanya klorofil yang larut. Gen matK parsial telah diisolasi dengan metode Polymerase Chain Reaction (PCR) menggunakan Primer Forward matK-1RKIM-f dan Primer Reverse matK-3FKIM-r. Analisis urutan nukleotida matK menghasilkan fragmen berukuran 843 pb. Kedua urutan nukleotida matK dari sampel tanaman leilem yang berasal dari Kauditan dan Tomohon menunjukkan hasil barcode DNA yang sama.MaturaseK is a protein encoded by matK gene which is located in plant chloroplast. The aim of this research was to determine the DNA barcode of leilem plant (Clerodendrum minahassae L.) based on matK nucleotides sequence. This research was done by isolating total DNA of leilem, amplified matK gene by PCR, sequencing the PCR product, and determined the DNA barcode of leilem. Total DNA of leilem plant was isolated by using the modified procedure from InnuPrep Plant DNA Kit. The DNA isolation resulted a green-yellowish solution which shows dissolved chlorophyll. Partial matK gene was amplified using PCR method with matK-1RKIM-f as forward primer and matK-3FKIM-r as reverse primer. Amplification by PCR resulted a 843 bp DNA fragment of matK. Both nucleotide sequences of matK from two samples of leilem plant taken from Kauditan and Tomohon showed the same DNA barcode.
Perbaikan Proses Pembuatan Gula Merah Aren di Pabrik Gula Aren Masarang Tomohon Pontoh, Julius; Wuntu, Audy
Jurnal MIPA Vol 3, No 2 (2014)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jm.3.2.2014.5316

Abstract

Pabrik gula aren Masarang Tomohon saat ini memiliki masalah rendahnya efisiensi produksi akibat pembentukan bongkahan gula selama tahap akhir pemprosesan gula. Tujuan penelitian ini adalah untuk meningkatkan efisiensi proses produksi gula di pabrik dengan penekanan pada sorpsi air oleh gula dan pada penghancuran bongkahan gula selama produksi serbuk gula aren (gula semut). Umur simpan gula merah aren diestimasi dengan kurva isoterm absorpsi menggunakan rumus Labuza. Penghancuran gula dilakukan mula-mula melalui penentuan jenis gilingan, diikuti dengan membuat desain gilingan dan membangun konstruksi gilingan. Perhitungan umur simpan gula menghasilkan angka 6,4 tahun umur simpan. Konstruksi gilingan gula dibuat didasarkan pada gilingan martil (hammer mill) dengan kapasitas untuk mengurangi sisa produk hingga 1,28 persen.The Masarang Palm Sugar Factory in Tomohon is currently facing to the problems of the low efficiency production due to the crumble formation during the last step of sugar processing and the question of the shelf life of the product. Therefore the goal of this research is to improve the palm sugar processing at the factory by focusing to the understanding the water sorption by the sugar and to break down the sugar crumbles during the brown sugar powder production.  The shelf life of the brown palm sugar was estimated by the isotherm absorption curve followed by application of Labuza formula.  The sugar milling was processed by firstly determined the milling type, followed by designing the mill and then constructed it.  The calculation of shelf life of the sugar product was found as 6.4 years.  The sugar milling was constructed based on the hammer mill with the capacity to reduce the crumbles as low as 1.28 percent.
Uji Daya Hambat Ekstrak Metanol Beberapa Jenis Porifera Terhadap Bakteri Escherichia coli dan Staphylococcus aureus Saroinsong, Megawati S.; Kandou, Febby E. F.; Papu, Adelfia; Singkoh, Marina F. O.
Jurnal MIPA Vol 3, No 2 (2014)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jm.3.2.2014.5989

Abstract

Penelitian ini bertujuan untuk menguji daya hambat dari ekstrak metanol beberapa jenis porifera terhadap pertumbuhan bakteri E. coli dan S. aureus. Pengujian aktivitas antibakteri menggunakan metode Kirby-Bauer, yaitu dilakukan dengan mengukur zona hambat di sekeliling cakram kertas. Ekstrak Haliclona sp dapat menghambat pertumbuhan S. aureus pada konsentrasi ekstrak 30%, 60% dan 90% dengan hasil pengukuran diameter zona hambat 13.50 mm, 20.67 mm dan 27.33 mm; serta menghambat pertumbuhan E. coli dengan diameter zona hambat 10.08 mm, 12.83 mm dan 14.17 mm. Daya hambat ekstrak Agelas sp terhadap S. aureus menunjukkan diameter zona hambat 8.33 mm dan hanya menunjukkan pada konsentrasi ekstrak 90%, sementara daya hambat ekstrak Agelas sp pada konsentrasi 30%, 60% dan 90% terhadap E. coli menunjukkan diameter zona hambat sebesar 7.67 mm, 10.17 mm dan 14.17 mm. Daya hambat Spheciospongia sp terhadap S. aureus dan E. coli hanya terlihat pada konsentrasi ekstrak sebesar 90% dengan diameter zona hambat adalah 8.42 mm dan 8.75 mm. Berdasarkan hasil yang diperoleh, dapat disimpulkan bahwa ekstrak Haliclona sp memiliki potensi aktivitas antibakteri yang dapat digunakan sebagai bahan dasar pembuatan obat antibiotik.This research aimed to test the inhibition capabilities of methanol extract from several kinds of Porifera on Escherichia coli and Staphylococcus aureus growth. The antibacterial activity test using the Kirby-Bauer method, which delivered by measuring the inhibition zone around paper disc. The extract of Haliclona sp can inhibit the S. aureus growth at 30%, 60% and 90% of extracts concentration with the measurement of inhibition zone diameters are 13.50 mm, 20.67 mm and 27.33 mm; also inhibit the E. coli growth with inhibition zone diameters are 10.08 mm, 12.83 mm and 14.17 mm. The inhibition capability of Agelas sp extract on S. aureus shows that the inhibition zone diameters is 8.83 mm and only appear at 90% of extracts concentration, meanwhile the inhibition capability of Agelas sp extract at concentration 30%, 60% and 90% on E. coli shows diameters of inhibition zone are 7.67 mm, 10.17 mm and 14.17 mm. The inhibition capability of Spheciospongia sp on S. aureus and E. coli only occurred at 90% of extracts concentration with inhibition zone diameters 8.42 mm and 8.75 mm. Based on the results, it can be assumed that extracts of Haliclona sp has a potential antibacterial activity that can be used as a basic ingredients for antibiotic medicine.
Identifikasi Zooplankton di Perairan Pulau Bunaken Manado Ruga, Lisa; Langoy, Marnix; Papu, Adelfia; Kolondam, Beivy
Jurnal MIPA Vol 3, No 2 (2014)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jm.3.2.2014.5856

Abstract

Perairan Pulau Bunaken merupakan objek wisata yang terdapat di Manado Sulawesi Utara dan merupakan daerah perlindungan laut. Perairannya dijaga agar tetap menunjang diversitas organisme di sekitar pantai dan menghasilkan nilai tambah dari segi estetika dan ketersediaan ikan-ikan yang menjadi sumber pencarian bagi masyarakat nelayan di pesisir. Salah satu indikator keberadaan ikan dan kesuburan perairan adalah adanya zooplankton. Tujuan dari penelitian ini adalah untuk mengidentifikasi zooplankton yang ada di perairan Pulau Bunaken, Manado. Pengambilan sampel zooplankton dalam penelitian ini dilakukan di empat stasiun. Stasiun penelitian ini dipilih berdasarkan tempat pemanfaatannya, yaitu di daerah tubir, daerah liang, daerah dermaga perkampungan dan daerah observasi. Pengambilan sampel plankton di lakukan menggunakan plankton net dan sampel kemudian di identifikasi di Laboratorium Biokonservasi Biologi FMIPA UNSRAT. Hasil penelitian menunjukkan bahwa zooplankton yang ditemukan di Pulau Bunaken Manado secara umum termasuk dalam 14 kelas dan 28 genus dengan jumlah 7.676 individu. Stasiun yang mempunyai jumlah kelas terbanyak adalah stasiun III dan IV yaitu sebanyak 11 kelas.The waters of Bunaken Island is a tourism area located in Manado, North Sulawesi, and is a protected marine area. It is conserved to support the diversity of organisms around the coast and result in added value in terms of aesthetics and availability of fish that became the source of income for fishermen in coastal communities. One of the indicators for the presence of fish and water fertility is zooplankton. The purpose of this study was to identify zooplankton in the waters of Bunaken Island, Manado. Zooplankton sampling was performed at four stations. The stations were selected based on the utilization, those are in the edge region, the canal, the village dock, and observation area. Plankton sampling was done by using a plankton net and samples collected were identified in the laboratory of Bioconservation, Departement of Biology Faculty of Sciences UNSRAT. The results showed that zooplankton found in Bunaken Island, Manado was generally included in 14 classes and 28 genus with the number of sample of 7,676 individuals. Stations that have the highest number of 11 classes were III and IV.

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