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INDONESIA
Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Published by Badan Pengkajian dan Penerapan Teknologi
ISSN : 24422606     EISSN : 2548611X     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
JBBI, Indonesian Journal of Biotechnology & Bioscience, is published twice annually and provide scientific publication medium for researchers, engineers, practitioners, academicians, and observers in the field related to biotechnology and bioscience. This journal accepts original papers, review articles, case studies, and short communications. The articles published are peer-reviewed by no less than two referees and cover various biotechnology subjects related to the field of agriculture, industry, health, environment, as well as life sciences in general. Initiated at the then Biotech Centre, the journal is published by the Laboratory for Biotechnology, the Agency for the Assessment and Application of Technology, BPPT.
Arjuna Subject : -
Articles 542 Documents
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IDENTIFIKASI MOLEKULER JERUK NIPIS TEGAL BERDASARKAN FRAGMEN GEN 18S RIBOSOMAL RNA Yumna Rahmadias Hanifa; Sri Pujiyanto; Rejeki Siti Ferniah; Hermin Pancasakti Kusumaningrum
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 8 No. 2 (2021): December 2021
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (831.43 KB) | DOI: 10.29122/jbbi.v8i2.4883

Abstract

Tegal’s lime is a lime that grows in the coastal areas with high salinity, and research on the molecular identification of Tegal’s lime has never been done before. This study aims to determine the molecular identity and its phylogenetic relationship with other oranges in GenBank. DNA isolation was carried out using CTAB. The best DNA purity was 1.883 while the highest DNA concentration was 464.83 ng/?L. DNA amplification was carried out using the PCR method which consisted of denaturation, annealing, and extension steps. DNA was electrophoresed with agarose gel. The DNA band size was 572 bp. The fraqment of 18S rRNA of Tegal’s lime has a greatest similarity of 99,64% with Citrus sinensis, while based on phylogenetic tree it has a closest relationship with C. aurantium (bootstrap value 33%). The alignment of Tegal’s lime against the C. aurantium sample showed that there were 2 gaps and 4 base changes, while the alignment with other oranges and C. medica as an ancestral showed that there were transition and transversion at the 130th and 560th nucleotide respectively. Jeruk nipis Tegal merupakan jeruk nipis yang tumbuh pada daerah pesisir yang memiliki salinitas cukup tinggi dan penelitian tentang identitas jeruk nipis Tegal masih belum pernah dilakukan sebelumnya. Oleh karena itu, penelitian ini bertujuan untuk mengetahui identitas jeruk nipis Tegal secara molekuler dan mengetahui hubungan kekerabatannya dengan jeruk lainnya pada GenBank. Isolasi DNA dilakukan dengan menggunakan CTAB. Kemurnian DNA terbaik yaitu 1,883 sedangkan konsentrasi DNA tertinggi adalah 464,83 ng/?L. Amplifikasi DNA dilakukan dengan menggunakan metode PCR yang terdiri dari tahap denaturasi, anealing, dan ekstensi. DNA dianalisis menggunakan elektroforesis gel agarosa. Ukuran pita DNA yang didapatkan yaitu 572 pb. Fragmen gen 18S rRNA jeruk nipis Tegal memiliki kesamaan yang paling tinggi dengan Citrus sinensis sebesar 99,64% sedangkan berdasarkan analisis filogenetik, memiliki hubungan kekerabatan yang paling dekat dengan C. aurantium (nilai bootstrap sebesar 33%). Hasil pensejajaran jeruk nipis Tegal terhadap sampel C. aurantium menunjukkan adanya 2 gap dan 4 perubahan basa sedangkan pensejajaran yang dilakukan antara jeruk nipis Tegal dengan jeruk lain dan jeruk nenek moyang C. medica menunjukkan adanya 1 transisi pada basa ke-130 dan 1 transversi pada basa ke-560.
A GLUTARIMIDE FROM THE INDONESIAN MARINE CYANOBACTERIUM Oscillatoria sp. Viqqi Kurnianda; . Khairunnisa; Sofyatuddin Karina; Sri Agustina; Nurfadillah Nurfadillah; Musri Musman
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 8 No. 2 (2021): December 2021
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (678.571 KB) | DOI: 10.29122/jbbi.v8i2.4889

Abstract

In continuous concern of marine resources relating to molecular structures that have biological activity around the western part of the Indonesian Archipelago, a marine cyanobacterium species Oscillatoria sp. was found in Sabang Island, Aceh. This study aimed at investigating the chemical and biological contents of Oscillatoria sp. The isolation of the target molecule was carried out based on bioassay-guided separation with several steps of chromatography. In addition, the evaluation of bioactivity was determined by the MTT assay protocol against the human pancreatic cancer Panc-1 cell line. The planar structure of the target molecule was determined by spectroscopic analysis. Results showed that the whole planar structure was identified as cycloheximide-4-acetate (1), a cycloimide class of molecule. Moreover, the relative stereochemistry was confirmed by nuclear overhauser effect (NOE) analysis. Therefore, the molecule was assigned as (2S*,4S*,6S*,7R*)-cycloheximide-4-acetate. The cytotoxicity evaluation of compound 1 showed IC50 value of 0.32 mM against the human pancreatic cancer cell. Dalam perhatian berkelanjutan terhadap sumber daya laut yang berkaitan dengan struktur molekul yang memiliki aktivitas biologis di sekitar bagian barat Kepulauan Indonesia, spesies sianobakteria Oscillatoria sp. ditemukan di Pulau Sabang, Aceh. Penelitian ini bertujuan melakukan investigasi kandungan kimiawi dan biologis dari Oscillatoria sp. Isolasi molekul target dilakukan melalui metode pemisahan berdasarkan uji bioaktivitas dengan menggunakan beberapa tahapan kromatografi. Selain itu, evaluasi bioaktivitas ditentukan oleh protokol uji MTT terhadap sel kanker manusia Panc-1. Struktur planar dari molekul target ditentukan dengan analisis spektroskopi. Hasilnya, struktur planar dari senyawa target diidentifikasi sebagai sikloheksimida-4-asetat (1), kelompok molekul sikloimida. Selanjutnya, relatif stereokimia dikonfirmasi oleh analisis nuclear overhauser effect (NOE). Oleh karena itu, molekul tersebut ditetapkan sebagai (2S*,4S*,6S*,7R*)-sikloheksimida-4-asetat. Evaluasi sitotoksisitas pada senyawa 1 menunjukkan nilai IC50 sebesar 0.32 mM terhadap sel kanker pankreas.
PENINGKATAN PRODUKSI BIOMASSA SEBAGAI STRATEGI JITU DALAM MEMPERCEPAT PRODUKSI BIODIESEL BERBASIS MIKROALGA DI INDONESIA Swastika Praharyawan
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 8 No. 2 (2021): December 2021
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (658.125 KB) | DOI: 10.29122/jbbi.v8i2.4892

Abstract

Microalgae have been widely known possessing excellent characteristics to be used as biodiesel feedstock on the commercial-scale production level. However, microalgal biodiesel is still not economically feasible to be mass-scale produced due to its high price compared to the fossil-based diesel. Therefore, many efforts have to be conducted in order to increase its feasibility. One of accurate ways to solve its constraint is maximizing the production of microalgal biomass which will increase its lipid and biodiesel productivity. The optimization of microalgal biomass production process could be conducted against the four main cultivation parameters, namely carbon dioxide (CO2) supplementation, growth medium composition, cultivation environmental condition, and growth factors/hormones addition. The operational of microalgal cultivation by applying the optimum value will maximize its biomass production which will eventually increase its biodiesel productivity. This review specifically discusses the aforementioned parameters, including its essential role and the way on how to optimize those parameters in gaining maximum microalgal biomass production. Mikroalga diketahui memiliki karakteristik unggul sebagai bahan baku potensial dalam produksi biodiesel skala komersial. Namun, hal itu masih terbentur oleh tingginya harga produksi bila dibandingkan dengan bahan bakar diesel berbasis fosil. Oleh karena itu, berbagai upaya untuk meningkatkan kelayakan ekonominya harus dilakukan. Salah satu langkah jitu dalam memecahkan permasalahan tersebut adalah memaksimalkan produksi biomassa mikroalga, sehingga produktivitas lipid dan produktivitas biodiesel dapat meningkat. Optimasi produksi biomassa mikroalga dilakukan terhadap empat parameter utama kultivasi, seperti suplementasi karbon dioksida (CO2), komposisi media pertumbuhan, optimasi kondisi lingkungan dan penambahan faktor/hormon pertumbuhan. Operasional kultivasi mikroalga pada kondisi optimal akan memaksimalkan produksi biomassanya hingga akhirnya dapat mencapai produktivitas biodiesel yang maksimal. Artikel tinjauan ini secara khusus membahas keempat parameter tersebut di atas, termasuk perannya dalam kultivasi mikroalga serta bagaimana mengoptimalkannya agar dapat menghasilkan biomassa yang maksimal.
PENGUJIAN POTENSI ALERGENITAS COAT PROTEIN OF SUGARCANE MOZAIC VIRUS PADA TANAMAN TEBU TRANSGENIK Avif Firdausy Septian; Intan Ria Neliana; Banun Kusumawardani; Bambang Sugiharto
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 8 No. 2 (2021): December 2021
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (577.095 KB) | DOI: 10.29122/jbbi.v8i2.4896

Abstract

Sugarcane resistant to sugarcane mosaic virus (SCMV) was developed by overexpression of gene for coat protein (CP). Therefore, this study aimed at investigating the potential allergenicity of CP-SCMV in transgenic sugarcane. Allergenicity was assessed by analysis in silico and in vitro. In silico analysis using AllergenOnline FASTA alignment of full-length CP-SCMV amino acid showed that the protein had no similarity with allergen protein. However, the alignment using 80 mer CP-SCMV showed over 35% similarity, but this result was considered as false positive. In silico analysis on digestion capability of protease found the cutting sites of CP-SCMV by pepsin, trypsin and chymotrypsin. This result was further confirmed by in vitro gastrointestinal digestion in that CP-SCMV was digested by pepsin and trypsin. Although CP-SCMV was less degraded by in vitro heat treatment and quantitatively underwent slight decrease after 30-minute heating on 90 ºC, the protein might lose its function. These results indicated that CP-SCMV was considered having no potential allergen in transgenic sugarcane resistant to SCMV. Tebu tahan sugarcane mosaic virus (SCMV) dirakit melalui overekspresi gen untuk coat protein (CP). Oleh karena itu, penelitian ini bertujuan menguji alergenitas CP-SCMV pada tebu transgenik. Pengujian alergenitas dilakukan melalui analisis in silico dan in vitro. Hasil analisis in silico dengan pensejajaran AllergenOnline FASTA full-length asam amino CP-SCMV menunjukkan tidak ada kesamaan dengan protein alergen. Namun demikian pada pensejajaran 80 mer, CP-SCMV mempunyai kemiripan di atas 35% dengan alergen, tetapi hasil ini memiliki kecenderungan positif palsu. Analisis in silico terhadap kemampuan cerna protease ditemukan adanya sisi pemotongan CP-SCMV oleh enzim pensin, trypsin dan chymotrypsin. Hasil ini dikonfirmasi lebih lanjut dengan analisis in vitro pencernaan gastrointestinal yang menunjukkan bahwa CP-SCMV terdegradasi oleh pepsin dan trypsin. Walaupun hasil analisis in vitro menunjukkan CP-SCMV kurang dipengaruhi oleh perlakuan panas dan hanya sedikit berkurang pada pemanasan 90 ºC selama 30 menit, tetapi mungkin fungsi protein telah rusak. Hasil penelitian ini menyimpulkan bahwa CP-SCMV pada tanaman tebu transgenik tahan virus tidak berpotensi sebagai alergen.
ANALISIS KOMPARATIF MINERAL MIKRO DAN ANTI NUTRISI PADA BERAS ANTARA PADI REKAYASA GENETIK DAN TETUANYA Enny Rimita Sembiring; Puspo Edi Giriwono; Satya Nugroho; Maggy Thenawidjaja Suhartono
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 8 No. 2 (2021): December 2021
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (470.888 KB) | DOI: 10.29122/jbbi.v8i2.4925

Abstract

Comparative analysis is important aspect in food safety of transgenic crops to determine the effect of transgene on nutritional and anti-nutritional contents. This research was aimed to determine the concentration of Fe, Zn, phytic acid, and anti-trypsin activity, then assess the equivalence between transgenic rice and non-transgenic rice. Fe and Zn concentration was carried out using energy-dispersive x-ray fluorescence spectrometry. Anti-nutritional phytic acid and anti-trypsin activity were performed using visible light spectrophotometry. The data obtained were statistically tested using Independent sample t-test. These results indicated that the concentration of Fe, Zn, phytic acid, and anti-trypsin activity in 6 transgenic rice events were equivalent to non-transgenic rice. In conclusion, the transgene did not affect Fe, Zn, phytic acid, and anti-trypsin activities in brown rice of 6 transgenic rice events carrying cry1B::cry1Aa fusion genes. Analisis komparatif adalah salah satu aspek penting dalam keamanan pangan tanaman produk rekayasa genetik (PRG) untuk mengetahui pengaruh transgen terhadap kandungan nutrisi dan anti nutrisi. Pada penelitian ini dilakukan analisis komparatif konsentrasi mineral mikro Fe, Zn, asam fitat, dan aktivitas anti tripsin pada beras pecah kulit dari 6 galur padi PRG pembawa fusi gen cry1B::cry1Aa terhadap padi non-PRG Rojolele tetuanya. Analisis konsentrasi mineral mikro Fe dan Zn menggunakan metode energy-dispersive x-ray fluorescence spectrometry (ED-XRF) dengan 3 ulangan. Analisis konsentrasi anti nutrisi asam fitat dan aktivitas anti tripsin dilakukan menggunakan metode spektrofotometri sinar tampak dengan 8 ulangan. Data yang diperoleh diuji secara statistik menggunakan Independent sample t-test. Hasil yang diperoleh menunjukkan konsentrasi mineral mikro Fe, Zn, asam fitat, dan aktivitas anti tripsin pada beras dari 6 galur padi PRG pembawa fusi gen cry1B::cry1Aa setara dengan padi non-PRG Rojolele tetuanya. Kesimpulannya bahwa transgen tidak mempengaruhi kandungan mineral mikro Fe, Zn, asam fitat, dan aktivitas anti tripsin pada beras dari 6 galur padi PRG pembawa fusi gen cry1B::cry1Aa.
INSULIN: PRODUKSI, JENIS, ANALISIS, DAN RUTE PEMBERIAN Dudi Hardianto
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 8 No. 2 (2021): December 2021
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (398.39 KB) | DOI: 10.29122/jbbi.v8i2.4929

Abstract

Diabetes mellitus (DM) is a metabolic disorder disease characterized by hyperglycemia due to insulin deficiency, insulin resistance, or both. In 2019, people with DM reached 463 million in the world and will increase to 700 million by 2045. The total global health costs for DM treatment reach the US $760 billion. This review aims to explain the production, types, analysis, and route of insulin delivery. Human insulin is the first recombinant protein produced using genetic engineering. Escherichia coli, Saccharomyces cerevisiae, and Pichia pastoris are commonly host cells for producing human insulin and analogues. Currently, human insulin and analogues have replaced insulin derived from animal pancreas extracts. Analysis of insulin can be carried out through immunoassays, chromatography, and electrochemical biosensors. The route of delivery of insulin can be through subcutaneous injection, nasal, oral, and transdermal. Insulin is the primary drug in patients with type 1 diabetes mellitus (T1DM). In some cases, type 2 diabetes mellitus (T2DM) requires insulin for its treatment. The development of insulin delivery routes will improve compliance, convenience, and treatment effectiveness for DM patients. Diabetes melitus (DM) merupakan penyakit kelainan metabolisme yang ditandai dengan hiperglikemia karena kekurangan insulin, resistensi insulin, atau keduanya. Pada tahun 2019, penderita DM mencapai 463 juta di dunia dan akan meningkat menjadi 700 juta pada tahun 2045. Total biaya Kesehatan global untuk penanganan DM mencapai US $760 miliar. Telaah ini bertujuan untuk menjelaskan tentang produksi, jenis, analisis, dan rute pemberian insulin. Insulin manusia merupakan protein rekombinan yang pertama diproduksi menggunakan rekayasa genetika. Escherichia coli, Saccharomyces cerevisiae, dan Pichia pastoris merupakan sel inang yang umum digunakan untuk memproduksi insulin manusia dan insulin analog. Saat ini, insulin manusia dan insulin analog telah menggantikan insulin yang berasal dari ekstrak pankreas hewan. Analisis insulin dapat menggunakan immunoassay, kromatografi, dan biosensor elektrokimia. Rute pemberian insulin dapat melalui injeksi subkutan, nasal, oral, dan transdermal. Insulin digunakan sebagai obat utama pada penderita diabetes melitus tipe 1 (DMT1). Dalam beberapa kasus, diabetes melitus tipe 2 (DMT2) memerlukan insulin untuk pengobatannya. Pengembangan rute pemberian insulin akan meningkatkan kepatuhan penderita DM dan efektivitas pengobatan.
Back Cover JBBI Vol 8, No 2, December 2021 .
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 8 No. 2 (2021): December 2021
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

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Abstract

Front Cover JBBI Vol 8, No 2, December 2021 .
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 8 No. 2 (2021): December 2021
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

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Preface JBBI Vol 8, No 2, December 2021: Foreword and Acknowledgement .
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 8 No. 2 (2021): December 2021
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

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Appendix JBBI Vol 8, No 2, December 2021: Keyword Index and Author Index .
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 8 No. 2 (2021): December 2021
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (130.053 KB)

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