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Jurnal Bioteknologi & Biosains Indonesia (JBBI)

JBBI
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Published by Badan Pengkajian dan Penerapan Teknologi

ISSN : 24422606 EISSN : 2548611X DOI : -

Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

JBBI, Indonesian Journal of Biotechnology & Bioscience, is published twice annually and provide scientific publication medium for researchers, engineers, practitioners, academicians, and observers in the field related to biotechnology and bioscience. This journal accepts original papers, review articles, case studies, and short communications. The articles published are peer-reviewed by no less than two referees and cover various biotechnology subjects related to the field of agriculture, industry, health, environment, as well as life sciences in general. Initiated at the then Biotech Centre, the journal is published by the Laboratory for Biotechnology, the Agency for the Assessment and Application of Technology, BPPT.

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Articles 542 Documents

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EKSTRAKSI DAN IDENTIFIKASI METABOLIT SEKUNDER DARI ISOLAT AL6 SERTA POTENSINYA SEBAGAI ANTIBAKTERI TERHADAP Escherichia coli Alfian Syarifuddin; Sodiq Kamal; Fitriana Yuliastuti; Missya Putri Kurnia Pradani; Ni Made Ayu Nila Septianingrum
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 2 (2019): December 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Extraction and Identification of Secondary Metabolites from AL6 IsolatesÂ and Its Potential as Antibacterial against Escherichia coliABSTRACTSecondary metabolites in the form of antibiotics can be produced by rhizospheric bacteria. AL6 bacterial isolate, which is one of the bacterial isolates from the rhosphere of Saccarum officinarum L., is known to produce antibiotic compounds. This study aims to determine the activity of antibiotics from AL6 ethyl acetate extracts produced by AL6 bacterial isolates, to analyze the minimum inhibitory concentration (MIC) and the similarity of the active substances using GCMS. The ethyl acetate extract obtained was tested for MIC at 1.25%, 2.5%, 5.0%, 10.0%, 20%, and 40% concentrations. Detection of potential antibiotic spots was carried out using bioautographic thin layer chromatography (TLC). Compounds responsible for antibiotic activity were analyzed using GCMS. Minimum inhibitory levels obtained reached 2.5%. The active spots responsible for antibiotic activity against Escherichia coli at Rf 0.94. Components detected using GCMS and suspected to be antibiotics include chloroform; ethane, 1,1-dimethoxy-(CAS) dimethyl acetal; dan 1,3-dioxolane, 2-methoxymethyl-2,4,5-trimethyl.Keywords: AL6 bacterial isolate; antibiotic; Escherichia coli; GCMS; MICABSTRAKMetabolit sekunder berupa antibiotik dapat diproduksi oleh bakteri rizosfer. Isolat bakteri AL6, salah satu isolat bakteri dari rizosfer Saccarum officinarum L., diketahui dapat menghasilkan senyawa antibiotik. Penelitian ini bertujuan mengetahui aktivitas antibiotik dari ekstrak etil asetat antibiotik AL6 yang dihasilkan isolat bakteri AL6, menganalisis kadar hambat minimum (KHM), serta kemiripan zat aktif menggunakan GCMS. Ekstrak etil asetat yang diperoleh diuji KHM-nya pada konsentrasi 1,25%, 2,5%, 5,0%, 10,0%, 20%, dan 40%. Deteksi bercak yang berpotensi sebagai antibiotik dilakukan menggunakan kromatografi lapis tipis (KLT) bioautografi. Senyawa yang berperan dalam aktivitas antibiotik dianalisis menggunakan GCMS. Kadar hambat minimal yang diperoleh mencapai 2,5%. Hasil uji KLT bioautografi memperlihatkan bercak aktif sebagai antibiotik terhadap Escherichia coli pada Rf 0,94. Komponen senyawa yang terdeteksi menggunakan GCMS dan diduga sebagai antibiotik antara lain chloroform; ethane, 1,1-dimethoxy-(CAS) dimethyl acetal; dan 1,3-dioxolane, 2-methoxymethyl-2,4,5-trimethyl.

Back Cover JBBI Vol 6, No 1, June 2019 Catur Sriherwanto
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 1 (2019): June 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

KONSENTRASI MALONDIALDEHID PADA TIKUS DIABETIK YANG DIBERI PAKAN BERBAHAN SAGU (Metroxylon sagu) DAN Moringa oleifera Rahmah Nadea Fitriyani Muhajirin; A'immatul Fauziyah; Avliya Quratul Marjan
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 2 (2019): December 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Malondialdehyde Concentration in Diabetic Rats Given Feed Made from Sagu (Metroxylon sagu) and Moringa oleiferaÂ ABSTRACTHyperglycemia in type 2 diabetes mellitus (DMT2) can cause oxidative stress characterized by increased malondialdehyde (MDA) production. Flavonoids have antioxidant activity that can suppress the production of free radicals that cause oxidative stress. Flavonoids are found in Cersa mori (CM), a food product made from sagu (Metroxylon sagu) and Moringa oleifera. This study aims to analyze the effect of CM administration on MDA levels in alloxan-induced diabetic rats. Antioxidant activity and total CM flavonoids were analyzed using DPPH reduction and colorimetry methods. Thirty-two Wistar rats were divided into 4 groups: negative control (K1), positive control 1 (K2), positive control 2 (K3) and treatment of feeding 5 g CM/200 g BW per day (K4). The intervention was carried out for 30 days. MDA levels were examined before and after the intervention by spectrophotometry. The results showed a significant decrease in MDA levels in K2, K3, and K4 by -11.5 Â± 3.39 Î¼M, -10.5 Â± 4.32 Î¼M, and -14.9 Â± 2.85 Î¼M, respectively. The lowest decrease in MDA levels was found in K4 fed with CM (p < 0.05).Keywords: alloxan; diabetes; flavonoid; malondialdehyde; resistant starchABSTRAKHiperglikemia pada diabetes melitus tipe 2 (DMT2) dapat menimbulkan stres oksidatif yang ditandai dengan peningkatan produksi malondialdehid (MDA). Flavonoid memiliki aktivitas antioksidan yang mampu menekan produksi radikal bebas penyebab stres oksidatif. Flavonoid terdapat di dalam produk pangan Cersa mori (CM) yang terbuat dari sagu (Metroxylon sagu) dan kelor (Moringa oleifera). Penelitian ini bertujuan menganalisis pengaruh pemberian CM terhadap kadar MDA tikus diabetes yang diinduksi aloksan. Aktivitas antioksidan dan total flavonoid CM masing-masing dianalisis menggunakan metode reduksi DPPH dan kolorimetri.Tiga puluh dua ekor tikus Wistar dibagi menjadi 4 kelompok: kontrol negatif (K1), kontrol positif 1 (K2), kontrol positif 2 (K3), dan perlakuan yang diberikan 5 g CM/200 g BB per hari (K4). Intervensi dilaksanakan selama 30 hari. Kadar MDA diperiksa sebelum dan setelah intervensi secara spektrofotometri. Hasil menunjukkan adanya penurunan signifikan kadar MDA pada K2, K3 dan K4 yaitu secara berturut-turut sebesar -11,5 Â± 3,39 ÂµM; -10,5 Â± 4,32 ÂµM; dan -14,9 Â± 2,85 ÂµM. Penurunan kadar MDA terendah terdapat pada K4 yang diberikan CM (p < 0,05).

PENGARUH SEREAL BERBAHAN SAGU DAN Moringa oleifera TERHADAP KADAR GLUKOSA DARAH TIKUS YANG DIINDUKSI ALOKSAN Melin Novidinisa Herada Putri; A'immatul Fauziyah; Taufik Maryusman
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 2 (2019): December 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

The Effects of Cereal Made From Sagu and Moringa oleifera on the Blood Glucose Level of Alloxan-Induced RatsDiabetes Mellitus (DM) type 2 could increase oxidative stress and blood glucose level. Resistant starch compounds in instant cereal Cersa Mori have antidiabetic properties. This research aimed to analyze the effect of Cersa Mori on fasting blood glucose (FBG) levels of diabetic white rats induced by alloxan. This is a true experimental study with a randomized pre-post control group design using 27 male Wistar strain rats divided into 3 groups randomly, i.e (KN) feed and distilled water, (KP) glibenclamide 0.126mg/200gBB/day, (P) Cersa Mori 5g/200gBB/day. KP and P groups were given alloxan 125 mg/KgBB subcutaneously and the intervention was carried out for 30 days. FBG level was measured using the GOD-PAP method. The results of Paired T-Test showed the effect of Cersa Mori on lowering FBG levels in hyperglycemic rats (P = 0,006). One-Way ANOVA test showed that Cersa Mori reduced FBG level, which was equivalent to those given glibenclamide (P = 0,366). It can be concluded that giving Cersa Mori 5g/200gBB/day for 30 days had a significant effect on lowering FBG level.Â Keywords: alloxan; Cersa Mori; diabetic rats; fasting blood glucose level; resistant strachABSTRAKDiabetes Mellitus (DM)tipe 2 dapat memicu stres oksidatif dan meningkatkan kadar glukosa darah puasa (GDP). Senyawa pati resisten dalam sereal siap saji Cersa Mori memiliki sifat antidiabetik. Penelitian ini bertujuan menganalisis pengaruh pemberian Cersa Mori terhadap kadar glukosa darah tikus putih diabetes yang diinduksi aloksan. Penelitian true-experimental ini menggunakan randomized pre-post control group design. Sampel sebanyak 27 ekor tikus jantan galur Wistar dibagi menjadi 3 kelompok secara acak yaitu; (KN) pakan dan akuades, (KP) glibenklamid 0,126 mg/200gBB/hari, (P) Cersa Mori 5g/200gBB/hari. KP sampai P diberikan aloksan 125 mg/KgBB secara subkutan dan intervensi dilakukan selama 30 hari. Pengukuran GDP menggunakan metode GOD-PAP. Hasil Uji-T menunjukkan pengaruh Cersa Mori dalam menurunkan GDP tikus hiperglikemia (P = 0,006). Uji ANOVA satu arah menunjukkan penurunan GDP pada kelompok Cersa Mori (P) setara dengan tikus yang diberi glibenklamid (P = 0,366). Dengan demikian dapat disimpulkan bahwa pemberian Cersa Mori dosis 5g/200gBB/hari selama 30 hari berpengaruh terhadap penurunan GDP secara signifikan.

Appendix JBBI Vol 6, No 1, June 2019: Keyword Index and Author Index Catur Sriherwanto
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 1 (2019): June 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Preface JBBI Vol 6, No 1, June 2019: Foreword and Acknowledgement Teuku Tajudin
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 1 (2019): June 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Front Cover JBBI Vol 6, No 1, June 2019 Catur Sriherwanto
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 1 (2019): June 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

RESPONS PERTUMBUHAN BIBIT KENTANG (Solanum tuberosum) TERHADAP FORMULASI BIOSTIMULAN BERBASIS Trichoderma spp. Winda Nawfetrias; Dwi Pangesti Handayani; Irna Surya Bidara; Armelia Tanjung
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 2 (2019): December 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Growth Responses of Potato (Solanum tuberosum) Plantlets upon ApplicationÂ of Different Formulations of Trichoderma spp.-Based BiostimulantPlantlet acclimatization is one of several critical steps in potato clonal seedling production which often hampers the availability of quality plant materials. Application of biostimulant formulation containing Trichoderma spp. may increase growth capability of potato plantlets due to the improvement of plantlets in absorbing nutrient from the growth media. The aim of this research was to obtain the best formulations of biostimulant containing several strains of Trichoderma spp. to be applied in the acclimatization stage of potato seeds. Nine formulations of biostimulant (i.e. S1, S2, S3, S4, S5, S6, S7, S8, S9) each of which contained three to four different strains of five Trichoderma spp. isolates, were mixed with organic substrate carriers. The biostimulants were then applied to potato plantlets cv. Atlantic at the beginning of acclimatization. Plantlets without biostimulant were used as the control treatment (S0). The results showed that the S8 biostimulant formulation significantly increased the growth of the potato plantlets. The results of this experiment indicated that compared with the control and other formulations, the S8 biostimulant formulation significantly increased the growth of potato plantlets based on the number of leaves and number of shoots.Keywords: aclimatitation; biostimulant; potato; seedling; TrichodermaABSTRAKAklimatisasi planlet adalah salah satu tahapan kritis dalam produksi bibit klonal kentang yang seringkali menghambat ketercukupan bahan tanaman berkualitas. Penggunaan produk berbasis Trichoderma spp. diduga dapat meningkatkan kemampuan planlet untuk menyerap unsur hara dari media tumbuh. Tujuan penelitian ini adalah untuk mengetahui formulasi biostimulan terbaik yang mengandung Trichoderma spp. yang diaplikasikan pada tahap aklimatisasi bibit kentang. Sembilan biostimulan (S1, S2, S3, S4, S5, S6, S7, S8, S9) diformulasikan menggunakan substrat pembawa organik dan masing-masing mengandung campuran tiga atau empat strain dari lima isolat Trichoderma spp. Kesembilan biostimulan tersebut kemudian diujikan pada planlet kentang varietas Atlantik siap aklimatisasi. Planlet tanpa aplikasi biostimulan digunakan sebagai kontrol (S0). Hasil percobaan ini menunjukkan bahwa dibandingkan dengan control maupun formulasi lainnya, formulasi biostimulan S8 secara signifikan meningkatkan pertumbuhan planlet kentang cv. Atlantik dilihat dari jumlah daun dan jumlah tunasnya.

Front Cover JBBI Vol 6, No 2, December 2019 . .
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 2 (2019): December 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Back Cover JBBI Vol 6, No 2, December 2019 . .
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 6 No. 2 (2019): December 2019
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

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