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BIOPROSPEK
Published by Universitas Mulawarman
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Veterinary
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Articles 77 Documents
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UJI DAYA HAMBAT CENDAWAN (Trichoderma spp.) TERHADAP CENDAWAN PATOGEN (Colletothricum capsici) PADA TANAMAN CABAI Anwar, Merdi Sahara; Martina, Ina; Fahmi, Syafrizal
BIOPROSPEK: Jurnal Ilmiah Biologi Vol 13 No 1 (2018): Bioprospek: Jurnal Ilmiah Biologi Volume 13 Number 1 2018
Publisher : Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (224.328 KB) | DOI: 10.30872/bp.v13i1.195

Abstract

Uji daya hambat cendawan (Trichoderma spp.) terhadap cendawan patogen (Colleotothricum capsici) pada tanaman cabai telah dilaksanakan pada bulan November 2017 sampai Januari 2018 di Laboratorium Biologi Balai Pengkajian Teknologi Pertanian, Kalimantan Timur. Penelitian ini bertujuan untuk mengetahui daya penghambatan cendawan antagonis Trichoderma spp. yang berasal dari tanah yang berpotensi menghambat pertumbuhan cendawan patogen Colleotothricum capsicipada tanaman cabai (Capsicum annum).Tahap penelitian ini meliputi pengambilan sampel tanah, sterilisasi alat, pembuatan media PDA, isolasi tanah, isolasi daun cabai, pembuatan biakan murnidan uji daya hambat. Hasil penelitian menunjukkan bahwa dari uji daya hambat cendawan Trichoderma spp. terhadap cendawan Coelototrhicum capsici cendawan Trichoderma spp. bersifat lebih menghambat pertumbuhan cendawan Colleotothricum capsici. Hasil pengujian didapatkan hasil bahwa pada cawan petri pertama persentase penghambatan sebesar 80%, cawan petri kedua sebesar 80%, cawan petri ketiga sebesar 75%, cawan petri keempat sebesar 75%, cawan petri kelima sebesar 75% dan cawan petri keenam sebesar 71%. Hal ini menunjukkan bahwa Trichoderma spp. merupakan jamur yang dapat menghambat atau menekan pertumbuhan jamur dari bagian daun pada tanaman cabai (Capsicum annum).
PENENTUAN KADAR LOGAM KADMIUM (Cd) PADA TANAMAN KANGKUNG AIR (Ipomoea aquatica Fork.) DAN SEDIMEN SEBAGAI MEDIA TUMBUHNYA DI SAMARINDA Indary, Melati Deshy; Susanto, Dwi; Sudrajat, Sudrajat
BIOPROSPEK: Jurnal Ilmiah Biologi Vol 11 No 2 (2016): Bioprospek: Jurnal Ilmiah Biologi Volume 11 Number 2 2016
Publisher : Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam

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Abstract

The purpose of this study is to determine of the concentration of heavy metal Cd in water spinach plants and in the sediments as a its growth medium. The sampling is done at several locations of water spinach suppliers such as Benanga reservoirs, Asparagus Bengkuring and the rear outdoor area of FKESMAS Unmul. Cd contect was analyzed using Atomic Absorption Spectrophotometry (AAS).The results showed that the content of Cadmium (Cd) in water spinach plants, which were taken from Benanga reservoir is 0.0022 ± 0.0003 mg Kg-1; Asparagus Bengkuring is 0.0021 ± 0.0004 mg Kg-1 ; the Rear outdoor area of FKESMAS Unmul is 0.0016 ± 0.0003 mg/kg and the average level of Cadmium in sediment, which were taken from reservoir Benanga is 0.442 ± 0.336 mg Kg-1; Asparagus Bengkuring is 0.513 ± 0.317 mg Kg-1; the Rear outdoor area of FKESMAS Unmul is 0.08 ± 0.039 mg Kg-1. The level of Cadmium in water spinach plant ranges from 0.0016 to 0.0022 mg Kg-1, which is under the limit determined by the BPOM (0.2 mg Kg-1). The level Cadmium in the plant is determined by the levels of heavy metals in sediment with regression coofficient 0.001X Y = 0.001x + 0.001) at significance level 0.008. The conclusion of this research is the levels of heavy metals in the water spinach plant originating from several suppliers locations in Samarinda meets the qualifications of hygienic vegetables set by BPOM. And, it also concluded that the level of heavy metals in water spinach is specified by the level of heavy metal in its sediment.
Efek Pemanis Buatan Sakarin Dan Sari Buah Naga Merah (Hylocereus costaricensiss) Terhadap Histopatologi Hati Dan Ginjal Mencit (Mus musculus L.) Sari, Ela Santi Mulya; Sudiastuti, Sudiastuti; Sudrajat, Sudrajat
BIOPROSPEK: Jurnal Ilmiah Biologi Vol 11 No 1 (2016): Bioprospek: Journal Ilmiah Biologi. Volume 11 Number 1 2016
Publisher : Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (194.178 KB) | DOI: 10.30872/bp.v11i1.129

Abstract

This study aims to determine the effects of saccharin and dragon fruit juice on histopatologic of liver and kidney and determine the effective dose of dragon fruit juice to improve the cells of liver and kidney mice (Mus musculus L.) after fed saccharin. The study design used was completely randomized design (CRD). In this study, the independent variables consist of two control and three treated treatment with 5 replications. The histological data of liver and kidneys of mice (Mus musculus L.) presented descriptively,and evaluation of differences in the structure of the liver and kidney mikroanatomi saccharin and dragon fruit (Hylocereus polyrhizus) be compared to normal structure. The results showed that administration of saccharin at a dose of 15mg/kg BB/day to given effect on the histopathology of kidney and liver of mice. Treatment of the red dragon fruit juice at a dose of 200mg/Kg BB/day may improve liver and kidney mikroanatomi structures damaged by the provision of saccharin. From this research, showed that the red dragon fruit juice swetened with saccharin can inhibition effects of on structure microanatomy the liver and kidney
EFEK HIPOGLIKEMIK EKSTRAK ETANOL BATANG KARAMUNTING (MELASTOMA MALABATHRICUM) TERHADAP KADAR GULA DARAH DAN PERUBAHAN STRUKTUR MIKROANATOMI PANKREAS MENCIT (MUS MUSCULUS L.) DIABETESI Wulandari, Anita; Sudiastuti, Sudiastuti; Sudrajat, Sudrajat
BIOPROSPEK: Jurnal Ilmiah Biologi Vol 11 No 2 (2016): Bioprospek: Jurnal Ilmiah Biologi Volume 11 Number 2 2016
Publisher : Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam

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Abstract

This study aims to determine the effect of ethanol extract of stem Karamunting to improve pancreatic microanatomy structure diabetic mice. The study design used was completely randomized design (CRD which is consisting of negative group control, positive control, and the group treated with 100,250 and 500 mg/kg weigth body dose of karamunting stem extract for 28 days. Blood glucose levels in mice then measured using the blood sugar measuring device gluco meter (Nesco Multicheck) Measurements were taken on day 7 after administration of alloxan. On the 28th day, treatment three at a dose of 500 mg / kg / day showed that the pancreatic cells as the cells in the positive control (normal), whereas treatment 1 at a dose of 100 mg / kg / day and treatment 2 doses 250 mg / kg / day is equal to the negative control. The results showed that the sample extraction rod microanatomy Karamunting able to improve the structure of the pancreas of mice at a dose of 500 mg / kg / day.
Morfologi Dan Morfometrik Spermatozoa Ayam Nunukan Ardhani, Fikri
BIOPROSPEK: Jurnal Ilmiah Biologi Vol 10 No 2 (2015): Bioprospek: Jurnal Ilmiah Biologi: Volume 10 Number 2 2015
Publisher : Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (544.928 KB)

Abstract

[Artikel telah ditarik] The fertility of a rooster is not only influenced by motility,viability and DNA integrity of the spermatozoa but it isdetermined by the morphological integrity of the spermatozoa.This study objectives to determine the morphological andmorphometric spermatozoa of nunukan rooster, one ofIndonesian germplasm. Rooster’s cement was collected usingdorso-abdominal massage method. The cement was stainedwith a combination of eosin Nigrosin. The stained cement wasused to observe the morphology and measurement of the mainparameters. The morphological was evaluated using amicroscope with a magnification of 100x as many as 100spermatozoa. The spermatozoa morphometric were analyzedusing the Raster Image program and its were identified the headdimension, the head length and width, the mid-piece length, thetail length, and the ratio of the head and tail. A nunukan roosterspermatozoon in average has total length of 86.724 ± 12.496μm with a head length, the mid-piece length, the tail length is12.766±2.307 μm, 4.220±1.127 μm, and 75.738 ± 9.061 μm,respectively, while the head width is 1.391±0.244 μm, and thethe head: the tail ratio is 1: 5.9. The morphologically normalpercentage is 80.50 ± 9.40 %. In conclusion, the characteristicsspermatozoa of nunukan rooster due to morphology,morphometric, and morphologically normal percentage aspectsare within the range of other types of rooster in many previousstudies.
Kadar Besi (Fe) pada Tanaman Bayam Cabut (Amaranthus tricolor L.) yang Ditanam pada Beberapa Media Tanah Bekas Galian Tambang Batubara di Samarinda Annisa Annisa; Dwi Susanto; Sudrajat Sudrajat
BIOPROSPEK: Jurnal Ilmiah Biologi Vol 11 No 1 (2016): Bioprospek: Journal Ilmiah Biologi. Volume 11 Number 1 2016
Publisher : Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (263.447 KB) | DOI: 10.30872/bp.v11i1.125

Abstract

This research aims to find out if there is a correlation between the availability of Fe on the soil of an excavated coal mine with Fe levels in spinach. This research out by experiment Completely Randomized Design consists of 5 treatment is P0 (soil media in Kebun Raya Unmul Samarinda), P1(soil media in Sambutan ), P2 (soil media in Batu Besaung ), P3 (soil media in Loa Bakung) and P4 (soil main excavated + soil media in Kebun Raya Unmul Samarinda with comparison 1:1) with repotition 4 unit polybag and each units consists of 10 seeds of spinach. The Plants maintained for 5 weeks and parameters measured is growth of plant height, leaf number, leaf colour, Fe content in the spinach and Fe content in this soil media. The results showed that the mine excavated soil with the average levels of Fe contains for 39,9598 µg/mL; 30,9458 µg/mL; 31,2599 µg/mL dan 28,502 µg/mL, consecutive to the origin of former soil mine samples is soil main excavated in Sambutan, Batu besaung, Loa bakung and soil mixture of all mines with the ground control (Kebun Raya Unmul). Soil main excavated is not good be used as a growing medium with the addition of fertilizer NPK. The availability of Fe elements in the soil followed by increased Fe levels in the spinach by the equation of guess regression y = 0,004x + 0,139 and value r = 0,902 on the significant levels 0,005.
STUDI KETERTARIKAN KECOAK JERMAN (Blattella germanica L.) PADA KARBOHIDRAT dari AMPAS TAHU dan AMPAS KELAPA Az, Syadza Zahratun Nufus; Hariani, Nova; Trimurti, Sus
BIOPROSPEK: Jurnal Ilmiah Biologi Vol 13 No 1 (2018): Bioprospek: Jurnal Ilmiah Biologi Volume 13 Number 1 2018
Publisher : Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (181.695 KB) | DOI: 10.30872/bp.v13i1.196

Abstract

Germany cockroaches (Blattella germanica L.) is one of the urban pest that has ability to breeding rapidly. The pest control methods, usually using fogging. However, this method increasing the resistance and environmental damage. So, the new inovation to pest control with bait method. Especially, gel bait is the most widely used method to controlling population of German cockroaches. In making gel bait using glucose aversion behavior that found on German cockroaches. These behavior causing the cockroaches aren’t interested in foods that contain glucose. The solution about behavior is to found another alternative food which is contain high carbohydrate and has abundant sources namely tofu and coconut dregs. This research aims to know the interest of German Cockroaches on tofu dregs and coconut dregs, to find out the total visits of German cockroaches on tofu dregs and coconut dregs, and to find out the relation of the total visits and total food consumption of German Cockroaches. This research using observation method on olfaktometer arena with observation time interval of 15 minutes, 30 minutes, 45 minutes, 60 minutes, and 120 minutes for five days. There are four treatments used in this research namely plain jelly, tofu dregs jelly, coconut dregs jelly, and combination jelly (made from tofu and coconut dregs). The results showed the German cockroaches are more interested in tofu dregs jelly than coconut dregs jelly. Total visits on tofu dregs jelly as much as 330 times and coconut dregs jelly as much as 114 times. The result of regression test showed a positive correlation and correlation between these two variable is significant.
ETNOMOTANI OBAT TRADISIONAL OLEH MASYARAKAT KUTAI DI KEC. MUARA BENGKAL KAB. KUTAI TIMUR Hadijah, Siti; Hendra, Medi; Hariani, Nova
BIOPROSPEK: Jurnal Ilmiah Biologi Vol 11 No 2 (2016): Bioprospek: Jurnal Ilmiah Biologi Volume 11 Number 2 2016
Publisher : Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (205.427 KB)

Abstract

This studied aim to find out the traditional medicinal plants that used by communities at Kutai area in the subdistrict of Muara Bengkal, how the Kutai community obtains a medicinal plants and which part of the plant that used as a traditional medicine. In this studied we used PRA (Participatory Rural Appraisal) methods, interview open-ended technique and the informant was determined by using snowball sampling. Stages of research include survey, observation, interviews, sampling, doing the identification of the herbarium and data analysis using Microsoft Excel 2007. There are 82 species of medicinal plants that are grouped into 42 families was found in this research. The Kutai community gained the medicinal plant by planted on their home garden, took on the field, looking for in the forest and bought in the market. The plant parts that used as a traditional medicine are the leaf, rhizome, stem, fruit, root, bark, seeds, tree sap, bulbs and the entire section.
PENGARUH FORMULA OBAT HERBAL UNTUK PENYEMBUHAN LUKA BAKAR Kastika, Suci Mayastik; Rahayu, Resti
BIOPROSPEK: Jurnal Ilmiah Biologi Vol 13 No 1 (2018): Bioprospek: Jurnal Ilmiah Biologi Volume 13 Number 1 2018
Publisher : Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (285.609 KB) | DOI: 10.30872/bp.v13i1.203

Abstract

This research was to determine the effect of herbal medicine’s formulation (Night Blooming Jasmine, Tumeric and Patchouli) with variation of night blooming jasmine’s concentration and oil for treating burnt injury. It had been carried out from October to November 2016. This research observed wound radius using Morton’s method to measure wound radius as well as the counting of leucocytes. The research showed that 4.00% concentration of night blooming jasmine enhanced the healing process as much as 82.89±5.49%. This concentration was proved in reducing leucocyte rate from 14,050/mm3 to 7,560/mm3 and fully regrew mice hairs well.
FITOREMEDIASI MENGGUNAKAN KAYU APU (Pistia stratiotes L.) DALAM KOLAM BEKAS TAMBANG BATUBARA TERHADAP PENYERAPAN LOGAM MANGAN (Mn) DAN KADMIUM (Cd) Purnama, Miryan Sucianti; Kusumawati, Eko; Susanto, Dwi
BIOPROSPEK: Jurnal Ilmiah Biologi Vol 13 No 1 (2018): Bioprospek: Jurnal Ilmiah Biologi Volume 13 Number 1 2018
Publisher : Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (203.319 KB) | DOI: 10.30872/bp.v13i1.204

Abstract

The aim of this research is to know the interaction that occurs on apu wood (Pistia stratiotes L.) in post-coal pool water and the ability of apu wood (Pistia stratiotes L.) on the absorption of manganese (Mn) and cadmium (Cd) from pond water post mining coal. This research use Factorial Randomized Completed Random (RAL) with 2 factors that is waste water concentration (100%, 75%, 50%, 25% and 0%) and variations in the weight of apu wood (Pistia stratiotes L.) (300 gr, 200 gr and 100 gr). The number of treatments was 20 treatment combinations. Each treatment was repeated 3 times. The data obtained were analyzed using SPSS (Statistical Product and Service Solutions) 16.0. The results show that apu wood is effective in lowering Mn and Cd metals against the pool of former coal mines. The effectiveness of Mn metal absorption by apu woods (Pistia stratiotes L.) indicated in all treatment combinations with a percentage of RE of 55.56-100% and the effectiveness of Cd metal absorption is shown in all treatment combinations with a percentage of RE of 81.81-100%.

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