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Animal Production : Indonesian Journal of Animal Production
Published by Universitas Jenderal Soedirman
ISSN : 14112027     EISSN : 25415875     DOI : https://doi.org/10.20884/1.jap.2021.23.2.10
Core Subject : Health, Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Veterinary
Animal Production is a peer-reviewed journal published by the Faculty of Animal Science, Jenderal Soedirman University in association with the Animal Scientist Society of Indonesia. The journal was established in 1999 and available online since May 4, 2011. Animal Production was initially published twice a year, then three times a year starting in 2005. The journal coverage focused on small scale livestock farming and technologies which includes the recent science development in animal production, including all aspects of nutrition, breeding, reproduction, post-harvest processing and socio-economics. All articles are available online. Full text available in pdf format and can be downloaded for free. Starting Vol 22 No 1 the papers have been and will be published in our new website (http://animalproduction.id), as the continuation of our previous but still active website (http://animalproduction.net).
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Hewan dan Zoologi
Articles 9 Documents
 1 
Search results for , issue " Vol 15, No 1 (2013): January" : 9 Documents clear
Effect of Substitution of Bovine Rumen Epithelial Tissue Scrapings for Fishmeal on the Growth Performance, Nutrient Utilization and Carcass Characteristics of Broiler Chickens Salami, SA; Ali, A; Babatunde, OT
ANIMAL PRODUCTION Vol 15, No 1 (2013): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract. An eight-week feeding trial was conducted to evaluate the performance, nutrient utilization and carcass characteristics of broiler chickens fed varied levels of bovine rumen epithelial tissue scrapings (BRETS) in replacement for fish meal at 0, 50 and 100% levels of substitution. Fifty four birds were used for the study and the birds were randomly distributed into three (3) dietary treatments which were randomly assigned to the rations. Feed and water were supplied ad libitum and data were collected on the feed intake, weight gain, feed conversion ratio and nutrient utilization. Data were also collected on the carcass characteristics after some birds had been slaughtered at the end of the experiment. Data collected were subjected to one-way analysis of variance and significant differences were indicated using Duncan multiple range test to separate the means. Results indicated that there was significant difference (P<0.05) in the feed intake, feed conversion ratio, nutrient utilization and cut-up parts, except for the lung and heart. However, there was no significant difference (P>0.05) in the weight gain, heart and lung weights. It was concluded that up to 50% BRETS inclusion as substitute for fish meal protein could be employed without any adverse effect, to maintain the quality of the broiler chicken while minimizing the high cost of whole fishmeal since BRETS is an abattoir by-product that is readily available locally. Keywords: Broiler chicken, epithelial tissue scrapings, fish meal, bovine rumen. Abstrak. Sebuah percobaan pakan selama delapan minggu dilakukan untuk mengevaluasi kinerja, pemanfaatan nutrisi dan karakteristik karkas ayam broiler yang diberi berbagai tingkat kerokan jaringan epitel (BRETS) rumen sapi sebagai pengganti tepung ikan pada 0 , 50 dan 100% tingkat substitusi. Lima puluh empat ayam broiler yang digunakan untuk penelitian dibagi secara acak ke dalam tiga (3) perlakuan pakan. Pakan dan air minum diberikan secara tak terbatas dan dicatat data konsumsi pakan, pertambahan bobot badan, rasio konversi pakan dan pemanfaatan nutrisi. Data lainnya yang diamati adalah karakteristik karkas setelah ayam dipotong pada akhir percobaan. Data yang dikumpulkan dianalisis dengan analisis ragam satu arah dan perbedaan nyata ditunjukkan dengan uji jarak berganda Duncan untuk memisahkan nilai tengah. Hasil penelitian menunjukkan bahwa ada perbedaan yang signifikan (P<0,05) pada konsumsi pakan, rasio konversi pakan, pemanfaatan nutrisi dan bagian-bagian potongan ayam, kecuali paru-paru dan jantung. Namun demikian, tidak ada perbedaan yang signifikan (P>0,05) pada bobot badan, jantung dan bobot paru-paru. Disimpulkan bahwa pemberian BRETS hingga 50 % sebagai pengganti protein tepung ikan dapat digunakan tanpa efek samping, untuk menjaga kualitas ayam broiler sambil menekan biaya tepung ikan yang mahal karena BRETS adalah hasil samping rumah pemotongan hewan yang tersedia secara lokal. Kata kunci: Ayam broiler, kerokan jaringan epitel rumen sapi, tepung ikan,SA Salami et al./Animal Production 15(1):15-23, January 2013 
Evaluation of the Effectiveness of Avian Influenza Vaccines in Native Chicken Using Two by Two Contingency Table Indradji, M; Sufiriyanto, .
ANIMAL PRODUCTION Vol 15, No 1 (2013): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

Abstract. The aim of this study was to evaluate the effectiveness of vaccination program analysis method using the 2 x 2 contingency table to provide better assessment to the vaccination program management and implementation. This study used survey methods. A total of 230 serum samples from vaccinated chickens and 20 serum samples from unvaccinated chickens were used. The blood serum samples were then examined with the Hemagglutination Inhibition Test/HI to measure antibody levels. The data were analyzed using a 2 x 2 contingency table. Results showed that the level of vaccine protection was 68.92% with 31.31% vaccines failure rate, the level of natural protective immunity in samples was 0%,  100% vaccines specificity and the effectiveness of the vaccine was 71.20%. Keywords: Vaccination, contingency table, hemaglutination inhibition. Abstrak. Penelitian bertujuan untuk mengevaluasi keberhasilan program vaksinasi dengan menggunakan tabel kontingensi 2 x 2 yang diharapkan dapat digunakan untuk menilai manajemen program vaksinasi yang lebih komprehensif. Penelitian ini menggunakan metode survei, dengan total 230 sampel serum dari ayam divaksinasi dan 20 sampel serum dari ayam yang tidak divaksinasi. Sampel serum darah ayam kemudian diperiksa dengan uji hemaglutinasi inhibisi/HI untuk mengukur tingkat antibodi. Data dianalisis dengan menggunakan tabel kontingensi 2 x 2. Hasil menunjukan bahwa tingkat proteksi vaksin yang diaplikasikan sebesar 68,92 % dengan  tingkat kegagalan vaksin 31,31 %. Tingkat kekebalan alami yang protektif pada sampel  sebesar 0 %, spesifitas vaksin 100 %, dan efektifitas  vaksin sebesar 71,20 %. Kata kunci: Vaksinasi, tabel kontingensi, inhibisi hemaglutinasi.M Indradji and Sufirianto/Animal Production 15(1):62-68, January 2013 
Fatty Acid Composition and Sensory Evaluation of The Meat of Broilers Fed Silkworm (Bombyx mori L) Pupa Dietary Supplementation Mentang, F; Jintasataporn, O; Ohshima, T
ANIMAL PRODUCTION Vol 15, No 1 (2013): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract.  The effects of silkworm pupa diets rich in n-3 fatty acids on fatty acid composition and quality of broiler meat were investigated. Diets containing varying amounts of dried or fresh pupa were prepared (1) basal contained 10 % fishmeal (control), (2) basal plus 10% of silkworm pupa-dried (SP), (3) basal plus 20% of silkworm pupa-dried (SP), (4) basal plus 5% of silkworm pupa-dried (SP) + 5% of silkworm pupa-fresh (SF), (5) basal plus 10% of silkworm pupa-dried (SP) + 10% of silkworm pupa-fresh (SF). One hundred and ninety five CPC 707 breed chickens of 21-days old were fed with the experimental diets. The prominent fatty acids in thigh meat tissue were oleic acid, palmitic acid, linoleic acid, stearic acid, and α-linolenic acid. Both the total amounts of n-3 fatty acids and polyunsaturated fatty acids increased, while the total amount of n-6 fatty acids decreased with increasing levels of silkworm meal in the feed, resulting in an increase in the n-3/n-6 ratio. There were no significant differences in the effects of the treatments on the sensory quality of cooked breast meat, with the exception of taste improvement, which significantly lower in the group that was fed a basal diet containing 5% each of dried and fresh pupa. In conclusion, application of silkworm pupa as fat source in feed could improve the taste and quality of broiler meat. Keywords: Silkworm pupa, omega-3, broiler, meat quality, fatty acid composition  Abstrak: Silkworm (Bombyx mori L.) pupa adalah by-product dari hasil industri ulat sutra yang mengandung omega-3 asam alpha linolenat (18:3-n3) tinggi, sekitar 40% dari total asam lemak. Penelitian ini bertujuan untuk menginvestigasi pengaruh pemberian silkworm pupa pada diet ayam broiler dalam komposisi asam lemak dan kualitas daging ayam. Lima jenis diet yang digunakan adalah (1) basal yang mengandung 10% tepung ikan (control), (2) basal yang mengandung 10% pupa kering (SP), (3) basal mengandung 20% pupa kering (SP), (4) basal mengandung 5% pupa kering (SP) dan 5% pupa basah (SF), dan (5) basal mengandung 10% pupa kering (SP) dan 10% pupa basah (SF). Penelitian ini menggunakan 195 ayam broiler jantan strain CPC 707 berumur 21 hari, pemberian diet dilaksanakan selama 21 hari. Hasil penelitian menunjukkan bahwa  asam oleat, palmitat, linoleat, stearat dan alpha linolenat merupakan asam-asam lemak yang dominan ditemukan pada daging paha ayam. Jumlah total omega-3 maupun asam lemak tak jenuh (PUFA) meningkat, sementara jumlah omega-6 menurun dengan penambahan silkworm pupa pada diet, menyebabkan perbandingan atau ratio n-3/n-6 menjadi tinggi. Dalam hal kualitas daging dada ayam, tidak ada perbedaan yang nyata  terlihat diantara kelima perlakuan diet, dengan pengecualian pada rasa daging ayam menunjukkan nilai sensory yang rendah pada perlakuan yang mengandung 5% pupa kering dan 5% pupa basah. Kesimpulan penelitian adalah silkworm pupa dapat dijadikan sebagai sumber omega-3 pada diet untuk meningkatkan kualitas dan rasa pada daging. Kata kunci: silkworm pupa, omega-3, broiler, kualitas daging, komposisi asam lemak.F Mentang et al./Animal Production 15(1):24-30, January 2013
The Efficacy of Methanol Extract of Garlic (Allium sativum) to Improve Rumen Fermentation Products Prayitno, Caribu Hadi; Hidayat, N
ANIMAL PRODUCTION Vol 15, No 1 (2013): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract.  This study was aimed to evaluate the effect of the increase levels of garlic extract (Allium sativum, As) in beef cattle feed on rumen fermentation products and microbial populations. The materials used  were rumen fluid and control feed (40 % fermented rice straw:60% concentrate). The research method was experimental using Randomized Complete Block Design (RCBD) applying 7 treatments which were repeated 3 times. The tested feed treatments were S0 (control feed), S1 (S0 + 1.5 ppm of Cr, S2 (S0 + 250 ppm of As extract), S3 (S1 + 250 ppm of As extract), S4 (S1 + 500 ppm of As extract), S5 (S1 +  750 ppm of As extract) and, S6 (S1  + 1000 ppm of As extract). The data were analyzed using analysis of variance and followed by Honestly Significant Difference test. The results showed that the treatment effects on total bacteria, protozoa population, total gas and methane were highly significant but had no effect on dry matter digestibilityDMD and organic matter digestibility OMD and production of VFA. The treatments increased total bacteria but decreased total gas and methane. The best result was achieved on treatment S6 (supplementation of As at the level of 1000 ppm in the feed) of which the feed with adequate Cr will result in the most efficient of fermentation.Keywords: Extract of Allium sativum, organic Cr, microbial population, rumen fermentation, beef cattleAbstrak. Penelitian ini bertujuan untuk mengevaluasi pengaruh dan level penambahan ekstrak  bawang putih (Allium sativum, As) dalam pakan  sapi potong terhadap  produk fermentasi dan populasi mikroba rumen. Materi yang digunakan dalam penelitian adalah cairan rumen sapi potong dan pakan kontrol (jerami fermentasi :  konsentrat; 40:60%). Metode penelitian adalah eksperimental menggunakan Rancangan Acak Kelompok (RAK) 7 perlakuan, setiap perlakuan diulang 3 kali. Pakan perlakuan yang diujicobakan pada sapi potong yaitu S0: pakan kontrol, S1: S0 + 1,5 ppm Cr, S2: S0 + 250 ppm ekstrak As, S3: S1 + 250 ppm ekstrak As, S4: S1 + 500 ppm ekstrak As, S5: S1 + 750 ppm ekstrak As, S6: S1 + 1000 ppm ekstrak As. Data dianalisis menggunakan analisis variansi dilanjutkan uji Beda Nyata Jujur. Hasil penelitian menunjukkan  perlakuan  berpengaruh terhadap total bakteri, populasi protozoa,  gas total dan metan tetapi  tidak berpengaruh terhadap kecernaan bahan kering dan bahan organik serta produksi VFA.  Perlakuan mampu meningkatkan total bakteri serta menurunkan gas total dan metan. Hasil terbaik, pada pakan perlakuan S6 yaitu suplementasi ekstrak As pada taraf 1000 ppm pada pakan yang tercukupi Cr organik menghasilkan efisiensi fermentasi yang paling baik.Kata kunci : Ekstrak Allium sativum, mineral mikro organik, populasi mikroba,  fermentasi rumen,  sapi potongCH Prayitno and N Hidyat/Animal Production 15(1):69-75, January 2013 
Doe Productivity of Boerawa Goat on Rural Condition Adhianto, K; Ngadiyono, N; Budisatria, I Gede Suparta; Kustantinah, Kustantinah
ANIMAL PRODUCTION Vol 15, No 1 (2013): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

Abstract. This research was carried out at Gisting sub-District, Tanggamus Regency, in order to find out doe productivity of Boerawa goat on rural on condition. This research involved 120 doe Boerawa goat housed in elevated barns. Measurements were performed to evaluate the litter size, birth weight, weight at weaning, and pre-weaning mortality. The results of the present research showed that birth weight of the kids was 3.10 kg and 2.94 kg for male and female, respectively. The average birth weight was based on the type of birth 3.20 kg and 3.04 kg for the single versus twin born kids. It was documented that the average weight at weaning of the kids were 17.12 kg and 16.23 kg for male and female, respectively. Whereas based on birth type, the average weight was of the single born kids and the twin at weaning were 17.30 versus 16.63 kg. In addition, doe reproduction index was 2.36 weaned kids/year, and the average doe productivity was 41.52 kg. Keywords: Boerawa goat, doe productivity, rural condition. Abstrak.  Penelitian ini dilakukan di Kecamatan Gisting Kabupaten Tanggamus, dalam rangka untuk mengetahui produktivitas induk kambing Boerawa pada kondisi pedesaan. Penelitian ini melibatkan 120 induk kambing Boerawa yang dipelihara di kandang panggung. Pengukuran dilakukan untuk mengevaluasi ukuran jumlah cempe sekelahiran, bobot lahir, bobot sapih, dan kematian pra-sapih. Hasil dari penelitian ini menunjukkan bahwa bobot lahir cempe adalah 3,10 kg dan 2,94 kg (masing-masing untuk jantan dan betina). Bobot lahir rata-rata didasarkan pada tipe kelahiran tunggal dan kembar adalah masing-masing 3.20 kg dan 3,04 kg. Diperoleh hasil bahwa bobot sapih adalah 17,12 kg dan 16,23 kg (masing-masing untuk jantan dan betina). Sedangkan berdasarkan tipe kelahiran, bobot sapih rata-rata dari tipe kelahiran tunggal dan kembar adalah 17.30 dan 16.63 kg. Selain itu, indeks reproduksi induk sebesar 2,36 cempe sapihan/tahun, dan produktivitas induk rata-rata adalah 41,52 kg. Kata kunci: Kambing Boerawa, produktivitas induk , kondisi pedesaan.K Adhianto et al./Animal Production 15(1):31-39, January 2013
Identification of MspI Polymorphism in the Forth Intron of Chicken Growth Hormone Gene and Their Associations with Growth Traits in Indonesia Native Chickens Mu’in, MA; Lumatauw, S
ANIMAL PRODUCTION Vol 15, No 1 (2013): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

Abstract. The objective of this research was to identify MspI polymorphism in the forth intron of chicken growth hormone (cGH) gene and their associations with growth traits in native Indonesian chickens. A total of 72 Indonesia native chickens were genotyped for a locus in the forth intron of cGH gene (cGH-I4/MspI locus) by PCR-RFLP with MspI restriction enzyme. The result showed two genotypes in this locus: AA and BB, with the frequency of 90.28% and 9.72%, respectively. Based on body weight average, B allele had a beneficial effect in increasing the live body weight. The result of General Linier Models analysis indicated that the polymorphism of this locus had significant association (P<0.05) with body weight at 4 months of age and so did the daily gain between 2 to 4 months of age. Therefore, these results suggest that there is a possibility of cGH-I4/MspI locus acting as a genetic marker for growth traits of native Indonesian chickens, especially for body weight at 4 months and daily gain between 2 to 4 months of age. Keywords: polymorphism, cGH gene, growth traits, Indonesia native chickens. Abstrak.  Tujuan dari penelitian ini adalah untuk mengidentifikasi polimorfisme MspI intron ke-4 pada gen hormon pertumbuhan ayam (cGH) dan hubungannya dengan sifat pertumbuhan pada ayam asli Indonesia. Sebanyak 72 ekor ayam asli Indonesia diidentifikasi gentotipenya untuk lokus pada intron ke-4 dari gen cGH (cGH-I4/MspI lokus) dengan PCR-RFLP mengguakan enzim restriksi MspI. Hasil penelitian menunjukkan adanya dua genotipe pada lokus ini: AA dan BB , dengan frekuensi masing-masing 90.28 % dan 9,72 %. Berdasarkan rata-rata bobot badan, alel B memiliki pengaruh yang menguntungkan dalam meningkatkan bobot badan hidup. Hasil analisis General Linier Model menunjukkan bahwa polimorfisme lokus ini memiliki hubungan yang nyata (P<0,05 ) dengan bobot badan pada umur 4 bulan dan begitu pula dengan pertambahan bobot badan harian antara umur 2 sampai 4 bulan. Oleh karena itu, hasil ini menunjukkan bahwa ada kemungkinan cGH-I4/MspI lokus bertindak sebagai penanda genetik untuk sifat pertumbuhan ayam asli Indonesia, terutama untuk bobot badan pada umur 4 bulan dan pertambahan bobot badan harian ayam antara umur 2 sampai 4 bulan. Kata kunci: polimorfisme , gen cGH , sifat pertumbuhan , ayam asli Indonesia.MA Mu’in and S Lumatauw/Animal Production 15(1):1-7, January 2013
Determination of Metabolizable Energy of Grape Pomace and Raisin Vitis Leaves Using in Vitro Gas Production Technique Moghaddam, M; Taghizadeh, A; Nobakht, A; Ahmadi, A
ANIMAL PRODUCTION Vol 15, No 1 (2013): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract. This present study was carried out to determine the fermentation characteristics, metabolizable energy (ME), organic matter digestibility (OMD), short chain fatty acids (SCFA), net energy for lactation (NEl) and volatile fatty acids (VFA) of grape pomace (GP) and raisin vitis leaves (RVL) using gas production technique. Two fistulated whether with average BW 45±2 kg were used. The data was analyzed using completely randomized design. The incubation times were 2, 4, 6, 8, 12, 16, 24, 36, 48 and 72 h. The gas production of soluble and insoluble fractions (a+b) were 289.49 and 249.93 mLg-1DM and the rate of gas production prices (c) were 0.015 and 0.024 (%/h) for RVL and GP, respectively. The ME, OMD, NEl and VFA for RVL were obtained 15.74 mjkg-1DM, 96.97%, 0.0505 Mcal/lb and 2.787 mmol, and for GP were achieved 13.63 mjkg-1DM, 87.04%, 0.496 Mcal/lb and 2.484 mmol, respectively, that showed significant differences (P<0.05). The amount of gas production in RVL (126.87 mLg-1DM) that was more than GP (112.10 mLg-1 DM) resulted low ME in GP compared to RVL. It was concluded, that the GP and RVL can be used in ruminants diets formulation.Keywords: metabolizable energy, gas production, grape pomace, vitis leaves Abstrak. Studi ini dilakukan untuk mengetahui karakteristik fermentasi , energi metabolis ( ME ) , kecernaan bahan organik ( OMD ) , asam lemak rantai pendek ( SCFA ) , energi bersih untuk laktasi ( nel ) dan asam lemak volatil ( VFA ) anggur pomace ( GP ) dan daun kismis Vitis ( RVL ) menggunakan teknik produksi gas . Dua berfistula apakah dengan rata-rata 45 ± 2 BW kg digunakan . Data dianalisis dengan menggunakan rancangan acak lengkap . Waktu inkubasi adalah 2 , 4 , 6 , 8 , 12 , 16 , 24 , 36 , 48 dan 72 jam . Produksi gas dari fraksi larut dan tidak larut (a + b ) adalah 289,49 dan 249,93 MLG - 1DM dan tingkat harga produksi gas ( c ) adalah 0,015 dan 0,024 ( % / jam ) untuk RVL dan GP , masing-masing. The ME , OMD , NEL dan VFA untuk RVL diperoleh 15,74 mjkg - 1DM , 96,97 % , 0,0505 Mcal / lb dan 2,787 mmol , dan untuk GP dicapai 13,63 mjkg - 1DM , 87.04 % , 0,496 Mcal / lb dan 2,484 mmol , masing-masing , yang menunjukkan perbedaan yang signifikan (P < 0,05). Jumlah produksi gas di RVL ( 126,87 MLG - 1DM ) yang lebih dari GP ( 112,10 MLG - 1 DM ) menghasilkan ME rendah di GP dibandingkan dengan RVL . Disimpulkan bahwa, GP dan RVL dapat digunakan dalam formulasi diet ruminansia. Kata kunci : inkubasi , produksi gas , anggur pomace , energi metabolis , daun vitis. M Moghaddam et al./Animal Production 15(1):40-46, January 2013
Microcapsule Application of Kecombrang Flower Extract: Effects of Concentration, Types of Fraction, pH of Medium, and NaCl on Microbiological Properties of Minced Beef Naufalin, R; Herastuti, SR
ANIMAL PRODUCTION Vol 15, No 1 (2013): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract. Kecombrang (Nicolaia speciosa Horan), in addition to  flavor and medicinal uses, it has potential as antimicrobial substances. The bioactive components in kecombrang are alkaloids, flavonoids, polyphenols, steroids, saponin and atsiri oils. This research was aimed to study the effectiveness of microcapsule from flower extract of kecombrang added to minced beef; the effects of pH interaction and NaCl addition on antimicrobial activity. The research used a Randomized Completely Block Design factorial pattern with 24 treatment combinations and 2 replications. The tested factors were type and microcapsule concentration ( control, ethanol extract  5%, etanol extract 10%, ethyl acetate extract 5% and ethyl acetate extract 10%), pH medium (pH 4 dan pH 7), and NaCl concentration (0% 2,5% and 5%). The results showed the microcapsule of flower extract of kecombrang that have antimicrobial activities in boiled minced beef was etyl acetate extract of 10% concentration under pH 4 and 5% NaCl . Keywords : Microcapsule, kecombrang, natural preservative, minced beef. Abstrak. Kecombrang (Nicolaia speciosa Horan) selain sebagai pemberi cita rasa dan berkhasiat obat, juga berpotensi sebagai antimikroba. Komponen bioaktif yang terdapat dalam kecombrang yaitu alkaloid, flavonoid, polifenol, steroid, saponin dan minyak atsiri. Penelitian ini bertujuan untuk mengetahui efektivitas mikrokapsul dari ekstrak bunga kecombrang yang ditambahkan pada daging sapi giling, pengaruh perlakuan pH dan penambahan NaCl terhadap potensi antimikroba.  Penelitian ini menggunakan Rancangan Acak Kelompok (RAK) factorial dengan  24 kombinasi perlakuan dan 2 kali ulangan.  Faktor yang diteliti adalah jenis dan konsentrasi mikrokapsul yaitu ( kontrol, ekstrak etanol  5%, ekstrak etanol 10%, ekstrak  etil asetat 5% dan  ekstrak etil asetat 10%), pH medium (pH 4 dan pH 7), dan konsentrasi NaCl (0% 2,5% dan 5%).  Hasil penelitian menunjukkan bahwa mikrokapsul ekstrak bunga kecombrang yang dapat berfungsi sebagai antimikroba pada daging giling rebus adalah ekstrak etil asetat  pada konsentrasi 10% dengan pengaturan pada pH  4 dan pada konsentrasi NaCl 5 %. Kata kunci :  Mikrokapsul, kecombrang, pengawet alami,  daging giling.R Naufalin and SR Herastuti/Animal Production 15(1):8-14, January 2013
Production of Progenies by Different Growth Hormone Genotypes (GH-MspI) of Their Parents Using PCR-RFLP in Ongole-crossbred Cattle Paputungan, U; Hakim, L; Ciptadi, G; Lapian, HFN
ANIMAL PRODUCTION Vol 15, No 1 (2013): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

Abstract. The objective of this study was to identify different growth hormone (GH) genotypes using MspI enzyme-restriction in parental cows and bulls mated by artificial insemination influencing body weight and average daily gain of their progenies. Total of 74 blood samples of Ongole-crossbred cows and their female progenies and 2 blood samples of bulls of Ongole breed were used in this study. Blood samples were screened for the presence of GH gene using PCR-RFLP method involving Msp1enzyme-restriction on 1.2 % agarose gel. To eliminate different age effects of the progenies, body weight data were adjusted for the 50 and 345days old of ages for the first and second weighing, respectively. Data were analyzed using statistical program in Excel XP. The results showed that various genetic factors of growth hormone Msp1 restricted enzyme had significant influence on growth performance and average daily gain of Ongole-crossbred progenies during 50 to 345 days of age. The heterozygous genotypes of the growth hormone Msp1+/- restricted enzyme excelled over their homozygous genotypes in respects of body weight gain. Therefore, the Msp1+/+, Msp1+/- and Msp1-/- genotypes can be used as the candidate genes in Ongole crossbred cattle to improve their body weight.Keywords: Ongole-crossbred cattle, body weight gain, growth hormone, Msp1 gene. Abstrak. Tujuan dari penelitian ini adalah untuk mengidentifikasi genotipe hormon pertumbuhan yang berbeda (GH) menggunakan pembatasan enzim MspI pada sapi induk dan pejantan yang dikawinkan melalui inseminasi buatan yang mempengaruhi bobot badan dan rata-rata pertambahan bobot badan harian keturunannya. Total 74 sampel darah sapi betina persilangan Ongole dan keturunan betinanya serta 2 sampel darah sapi pejantan bangsa Ongole digunakan dalam penelitian ini. Sampel darah diperiksa untuk kehadiran gen GH menggunakan metode PCR-RFLP yang melibatkan pembatasan enzim Msp1 pada gel agarosa 1,2 %. Untuk menghilangkan efek umur yang berbeda dari keturunan, data bobot badan disesuaikan ke arah 50 dan 345 hari untuk masing-masing penimbangan pertama dan kedua. Data dianalisis dengan menggunakan program statistik pada Excel XP. Hasil penelitian menunjukkan bahwa berbagai faktor genetik hormon pertumbuhan enzim terbatas Msp1 memiliki pengaruh yang signifikan terhadap kinerja pertumbuhan dan rata-rata pertambahan bobot badan harian keturunan persilangan Ongole pada umur 50-345 hari. Genotipe heterozigot dari hormon pertumbuhan enzim terbatas Msp1 + / - mengungguli genotipe homozigot mereka dalam hal penambahan bobot badan tubuh. Oleh karena itu, genotipe Msp1 + / + , Msp1 + / - dan Msp1 - / - dapat digunakan sebagai gen bakal pada sapi persilangan Ongole untuk meningkatkan bobot badan mereka. Kata kunci: Sapi persilangan Ongole, pertambahan bobot badan, hormon pertumbuhan, gen Msp1.U Paputungan et al./Animal Production 15(1):53-61, January 2013

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